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See discussions, stats, and author profiles for this publication at: https://www.researchgate.net/publication/308033564 Invited speaker presentation (DCU) Presentation April 2016 CITATIONS READS 0 39 1 author: Saidhbhe L. O'Riordan

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See discussions, stats, and author profiles for this publication at: https://www.researchgate.net/publication/308033564

Invited speaker presentation (DCU)

Presentation · April 2016

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1 author: Saidhbhe L. O'Riordan California Institute of Technology

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ELECTROCHEMICAL METHODS TO INVESTIGATE DISORDERS OF THE BRAIN AND EVALUATE MUSCLE TISSUE VIABILITY; REAL-TIME MONITORING OF PHYSIOLOGICAL BIOMARKERS.

Dr. Saidhbhe O’Riordan,

14th Apr, 2016

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Outline

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What?

Hydrogen peroxide (H2O2), Oxygen (O2)

Where?

Brain H2O2 , Muscle tissue O2

Who?

Rodents H2O2 and O2, Clinical O2

How?

Electrochemical real-time, instantaneous information

Why?

Pathogenesis, Diagnostic Animal models of disease e.g. PD, patients ischemia

14th April 2016 | Dr. Saidhbhe O’Riordan | DCU CASi

SLIDE 4

In-vitro

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14th April 2016 | Dr. Saidhbhe O’Riordan | DCU CASi

SLIDE 5

Sensors (in-vivo)

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14th April 2016 | Dr. Saidhbhe O’Riordan | DCU CASi

SLIDE 6

L.I.V.E. (in-vivo)

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14th April 2016 | Dr. Saidhbhe O’Riordan | DCU CASi

SLIDE 7

In-vivo characterisation of a dual catalase- based biosensor for the real-time electrochemical detection of brain hydrogen peroxide in freely-moving animals.

SL O’Riordan1 and JP Lowry1

1Neurochemistry Research Unit, BioAnalytics Laboratory, Department of Chemistry, Maynooth University, Maynooth, Co. Kildare, Ireland.

MANUSCRIPT IN PROGRESS

SLIDE 8

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Sensitivity (MCS)

Baseline 1.35 ± 0.25 nA Max 1.65 ± 0.31 nA (P = 0.0414*) n = 3 10 20 30 40 50 60 70 0.9 1.0 1.1 1.2 1.3 1.4

Time, min I, nA

Baseline MCS max. 0.0 0.5 1.0 1.5 2.0 2.5

I, nA

14th April 2016 | Dr. Saidhbhe O’Riordan | DCU CASi

SLIDE 9

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Selectivity (AA)

Baseline 0.95 ± 0.38 nA Max 1.04 ± 0.42 nA (P = 0.1043) n = 6

5 10 15 20 25 30 0.6 0.7 0.8 0.9 1.0

Time, min I, nA

Baseline AA max. 0.0 0.5 1.0 1.5 2.0

I, nA

14th April 2016 | Dr. Saidhbhe O’Riordan | DCU CASi

SLIDE 10

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Stability (in-vivo)

Baseline stability 5-6 am, 12 days (P = 0.9185) n = 7

2 4 6 8 10 12 0.0 0.5 1.0 1.5

Time (days) I, nA

14th April 2016 | Dr. Saidhbhe O’Riordan | DCU CASi

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Clinical O2 sensor

Translational research from benchtop to bedside

14th April 2016 | Dr. Saidhbhe O’Riordan | DCU CASi

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Clinical O2

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14th April 2016 | Dr. Saidhbhe O’Riordan | DCU CASi

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Clinical O2

Sensitivity 1.36 ± 0.045 nA/µM R2 =0.99 n = 3

14th April 2016 | Dr. Saidhbhe O’Riordan | DCU CASi

5 10 15 20 25 30 35 100 200 300 400 500 25M 50M 75M 100M 125M

Time, min I, nA

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Conclusion

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Hydrogen peroxide

Characterised Oxidative stress monitoring Animal models

Clinical O2

Rodent brain/periphery Proof of concept Human participants

Mr. Jack Kelly MB, FRCI, MD, FRCS (Plast) GUH

14th April 2016 | Dr. Saidhbhe O’Riordan | DCU CASi

SLIDE 15

Acknowledgements

Prof. John Lowry
Mr. Jack Kelly
Neurochemistry Research Unit (MU)
Dept. of Chemistry
Health Research Board (O2)
Wexford County Council (H2O2)

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