See discussions, stats, and author profiles for this publication at: https://www.researchgate.net/publication/308033564 Invited speaker presentation (DCU) Presentation · April 2016 CITATIONS READS 0 39 1 author: Saidhbhe L. O'Riordan California Institute of Technology 10 PUBLICATIONS 83 CITATIONS SEE PROFILE All content following this page was uploaded by Saidhbhe L. O'Riordan on 13 September 2016. The user has requested enhancement of the downloaded file.
ELECTROCHEMICAL METHODS TO INVESTIGATE DISORDERS OF THE BRAIN AND EVALUATE MUSCLE TISSUE VIABILITY; REAL-TIME MONITORING OF PHYSIOLOGICAL BIOMARKERS. Dr. Saidhbhe O’Riordan, 14 th Apr, 2016
Outline • What? Hydrogen peroxide (H 2 O 2 ), Oxygen (O 2 ) • Where? Brain H 2 O 2 , Muscle tissue O 2 • Who? Rodents H 2 O 2 and O 2 , Clinical O 2 • How? Electrochemical real-time, instantaneous information • Why? Pathogenesis, Diagnostic Animal models of disease e.g. PD, patients ischemia 14 th April 2016 | Dr. Saidhbhe O’Riordan | DCU CASi maynoothuniversity.ie
In-vitro 14 th April 2016 | Dr. Saidhbhe O’Riordan | DCU CASi maynoothuniversity.ie
Sensors ( in-vivo ) 14 th April 2016 | Dr. Saidhbhe O’Riordan | DCU CASi maynoothuniversity.ie
L.I.V.E. ( in-vivo ) 14 th April 2016 | Dr. Saidhbhe O’Riordan | DCU CASi maynoothuniversity.ie
In-vivo characterisation of a dual catalase- based biosensor for the real-time electrochemical detection of brain hydrogen peroxide in freely-moving animals. SL O’Riordan 1 and JP Lowry 1 1Neurochemistry Research Unit, BioAnalytics Laboratory, Department of Chemistry, Maynooth University, Maynooth, Co. Kildare, Ireland. MANUSCRIPT IN PROGRESS
Sensitivity (MCS) 1.4 Baseline 1.3 1.35 ± 0.25 nA Max 1.2 I, nA 1.65 ± 0.31 nA ( P = 0.0414 * ) 1.1 2.5 * n = 3 2.0 1.5 1.0 I, nA 1.0 0.5 0.9 0.0 Baseline MCS max. 0 10 20 30 40 50 60 70 Time, min 14 th April 2016 | Dr. Saidhbhe O’Riordan | DCU CASi maynoothuniversity.ie
Selectivity (AA) 2.0 1.5 1.0 Baseline I, nA 1.0 0.95 ± 0.38 nA 0.5 0.9 Max 0.0 1.04 ± 0.42 nA Baseline AA max. I, nA ( P = 0.1043) 0.8 n = 6 0.7 0.6 0 5 10 15 20 25 30 Time, min 14 th April 2016 | Dr. Saidhbhe O’Riordan | DCU CASi maynoothuniversity.ie
Stability ( in-vivo ) 1.5 Baseline stability 5-6 am, 12 days ( P = 0.9185) 1.0 n = 7 I, nA 0.5 0.0 0 2 4 6 8 10 12 Time (days) 14 th April 2016 | Dr. Saidhbhe O’Riordan | DCU CASi maynoothuniversity.ie
Clinical O 2 sensor Translational research from benchtop to bedside 14 th April 2016 | Dr. Saidhbhe O’Riordan | DCU CASi
Clinical O 2 14 th April 2016 | Dr. Saidhbhe O’Riordan | DCU CASi maynoothuniversity.ie
Clinical O 2 100 M 125 M 75 M Sensitivity 500 1.36 ± 0.045 nA/µM R 2 =0.99 400 50 M n = 3 25 M 300 I, nA 200 100 0 0 5 10 15 20 25 30 35 Time, min 14 th April 2016 | Dr. Saidhbhe O’Riordan | DCU CASi maynoothuniversity.ie
Conclusion • Hydrogen peroxide Characterised Oxidative stress monitoring Animal models • Clinical O 2 Rodent brain/periphery Proof of concept Human participants Mr. Jack Kelly MB, FRCI, MD, FRCS (Plast) GUH 14 th April 2016 | Dr. Saidhbhe O’Riordan | DCU CASi maynoothuniversity.ie
Acknowledgements • Prof. John Lowry • Mr. Jack Kelly • Neurochemistry Research Unit (MU) • Dept. of Chemistry • Health Research Board (O 2 ) • Wexford County Council (H 2 O 2 ) View publication stats View publication stats
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