Inducing a thermogenic response to cold shock in Escherichia coli - - PowerPoint PPT Presentation

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Inducing a thermogenic response to cold shock in Escherichia coli - - PowerPoint PPT Presentation

Aug 25, 2022 229 likes •436 views

Inducing a thermogenic response to cold shock in Escherichia coli Georgia Institute of Technology November 18, 2010 Heat generating gene: AOX intermembrane space matrix OmpA Witho thout Om OmpA AOX AOX AOX AOX AOX AOX AOX AOX

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Georgia Institute of Technology November 18, 2010

Inducing a thermogenic response to cold shock in Escherichia coli

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Heat generating gene: AOX

matrix intermembrane space

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AOX AOX AOX AOX

Witho thout Om OmpA

AOX AOX AOX AOX AOX AOX

OmpA

With th Om OmpA

AOX AOX

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Generating Heat Output Cold Shock Response

RN RNA Polym ymera rase

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288.102

288.096 288.150

288.000

Temperature Profile In E. coli (K) Temperature Profile in Agarose (K)

T(x)E.coli = −25091.379x 2 + 3.517x + 288.102 T(x)Agarose = −21.586x + 288.107

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288. 288.00 000 288. 288.06 065

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HybB-mRFP HybB-OmpA-mRFP

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14.5 14.7 14.9 15.1 15.3 15.5 15.7 15.9 16.1 16.3 16.5 Temper erature ( e (C) Min Minut utes

Absolute Te Tempe mperature b by Strai rain at n at 15 15° C

AOX1a RFP control

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21.5 22 22.5 23 23.5 24 24.5 25 25.5 Temper erature ( e (C) Min Minut utes

Absolute Te Tempe mperature b by Strai rain at n at 25 25° C

AOX1a RFP control

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  • 0.5

0.5 1 1.5 2 2.5 Degrees ees ( (C) Min Minut utes

Heat at Pro Production Above Base aseline ne A After C Cold S Shock ck

AOX1a (15 C) RFP control (15 C) AOX1a (20 C) RFP control (20 C)

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Conclusion

Project Design Modeling Construction Experiments

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