Flight iGEM NEFU_China Yao Li Attitudes towards creating a wiki - PowerPoint PPT Presentation

Flight iGEM NEFU_China Yao Li

Attitudes towards creating a wiki 40 30 Teams 20 10 0 Hard Tedious Easy Pleasant

Attitudes towards creating a wiki 40 30 Teams 20 77% 10 0 Hard Tedious Easy Pleasant

Average lines per wiki 12,000 9,000 Lines 6,000 3,000 0 2011 2012 2013 2014

Average lines per wiki 12,000 9,000 Lines 8,231 lines = 211 A4 pages 6,000 3,000 0 2011 2012 2013 2014

Q. Does creating a wiki need programming skill? Yes No 48% 52%

What’s Flight iGEM? Content Page Flight management builder iGEM

What’s Flight iGEM Flight iGEM iGEM Server

What’s Flight iGEM Create e t a e r C Flight iGEM iGEM Server e t a e r C

What’s Flight iGEM Create Edit e t a e r C Edit Flight iGEM iGEM Server e t a e r C

What’s Flight iGEM Flight iGEM iGEM Server

What’s Flight iGEM Flight iGEM iGEM Server

What’s Flight iGEM Flight iGEM iGEM Server

What’s Flight iGEM Flight iGEM iGEM Server

With Flight iGEM

With Flight iGEM

With Flight iGEM

With Flight iGEM

With Flight iGEM

With Flight iGEM

Improvement

Improvement Without Flight iGEM ! � Create templates ! � Prepare documents ! � Upload images Update images’ address Merge document into template Upload code

Improvement Without Flight iGEM With Flight iGEM ! ! � � Create templates Create templates ! � Create templates ! ! � � Prepare documents Prepare documents ! � ! ! Prepare documents � � Upload images Upload images ! � Update images’ address Update images’ address Fill form Merge document into template Merge document into template Click Upload code Upload code

Structure of Flight iGEM Three engines, for: 1. Content management: online collaboration platform 2. Template: page builder 3. Carriage: page carrier

Summary of Flight iGEM • PHP based & open-sourced platform • User-friendly interface • Automatic page builder • Online collaboration with version control Saving time!!! •

Efficiency Flight iGEM

Efficiency - Flight iGEM + 0 20 40 60 80 Days

Efficiency - 75 Flight iGEM + 0 20 40 60 80 Days

Efficiency - 75 Flight iGEM + 10 0 20 40 60 80 Days

Present

Present

Will you use our platform? No 6% Yes 94% Present

Achievements • We presented a Poster and a Talk. • We create our wiki with: 1. an Attributions page; 2. documented our standard BioBrick Parts and submitted them to the iGEM Registry; 3. documented parts; • Experimentally validate at least 4 new BioBrick Parts and submit them to the iGEM Parts Registry. • We integrated the investigated issues into the design and execution of our project for both Yogurt Guarder and the Flight iGEM. • Help 2 iGEM teams to build their wiki using the Flight iGEM. • Human Practices: visited a diary factory, conducted survey, educated the public for proper yogurt storage. • iGEM Special Prize: we created a Software Tool to simplify iGEM wiki building.

Reference 1. Ascenso OS, et al. (2011) An efficient synthesis of the precursor of AI-2, the signalling molecule for inter-species quorum sensing. Bioorganic & medicinal chemistry 19(3):1236-1241. 2. Bao Y, et al. (2013) Methylthioadenosine/S-adenosylhomocysteine nucleosidase (Pfs) of Staphylococcus aureus is essential for the virulence independent of LuxS/AI-2 system. International journal of medical microbiology : IJMM 303(4):190-200. 3. Blair WM & Doucette GJ (2013) The Vibrio harveyi bioassay used routinely to detect AI-2 quorum sensing inhibition is confounded by inconsistent normalization across marine matrices. Journal of microbiological methods 92(3):250-252. 4. Cappuyns AM, Bernaerts K, Vanderleyden J, & Van Impe JF (2009) A dynamic model for diauxic growth, overflow metabolism, and AI-2-mediated cell-cell communication of Salmonella Typhimurium based on systems biology concepts. Biotechnology and bioengineering 102(1): 280-293. 5. Galloway WR, Hodgkinson JT, Bowden SD, Welch M, & Spring DR (2011) Quorum sensing in Gram-negative bacteria: small-molecule modulation of AHL and AI-2 quorum sensing pathways. Chemical reviews 111(1):28-67. 6. Gamby S, et al. (2012) Altering the communication networks of multispecies microbial systems using a diverse toolbox of AI-2 analogues. ACS chemical biology 7(6):1023-1030. 7. Ha JH, et al. (2013) Crystal structures of the LsrR proteins complexed with phospho-AI-2 and two signal-interrupting analogues reveal distinct mechanisms for ligand recognition. Journal of the American Chemical Society 135(41):15526-15535. 8. Pereira CS, McAuley JR, Taga ME, Xavier KB, & Miller ST (2008) Sinorhizobium meliloti, a bacterium lacking the autoinducer-2 (AI-2) synthase, responds to AI-2 supplied by other bacteria. Molecular microbiology 70(5):1223-1235. 9. Pereira CS, Thompson JA, & Xavier KB (2013) AI-2-mediated signalling in bacteria. FEMS microbiology reviews 37(2):156-181.

Acknowledgement Prof. Guangchao Sui Lecturer: Shengnan Zhu Prof. Yuhua Li Lecturer: Chen Li Prof. Chunbo Teng iGEM Teams: NUDT_CHINA, Prof. Jingying Wang NJU-CHINA, Peking Northeast Forestry University Harbin Institute of Technology National Natural Science Foundation of China School of Forestry, Northeast Forestry University School of Science, Northeast Forestry University College of Life Sciences, Northeast Forestry University