Flight iGEM NEFU_China Yao Li Attitudes towards creating a wiki - - PowerPoint PPT Presentation

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Flight iGEM NEFU_China Yao Li Attitudes towards creating a wiki - - PowerPoint PPT Presentation

Flight iGEM NEFU_China Yao Li Attitudes towards creating a wiki 40 30 Teams 20 10 0 Hard Tedious Easy Pleasant Attitudes towards creating a wiki 40 30 Teams 20 77% 10 0 Hard Tedious Easy Pleasant Average lines per wiki


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Flight iGEM

NEFU_China Yao Li

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Teams

10 20 30 40 Hard Tedious Easy Pleasant

Attitudes towards creating a wiki

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Teams

10 20 30 40 Hard Tedious Easy Pleasant

Attitudes towards creating a wiki

77%

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Lines

3,000 6,000 9,000 12,000 2011 2012 2013 2014

Average lines per wiki

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Lines

3,000 6,000 9,000 12,000 2011 2012 2013 2014

Average lines per wiki

8,231 lines = 211 A4 pages

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No 52% Yes 48%

  • Q. Does creating a wiki need programming skill?
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What’s Flight iGEM?

Content management Page builder Flight iGEM

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What’s Flight iGEM

Flight iGEM

iGEM Server

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What’s Flight iGEM

Flight iGEM

iGEM Server

Create C r e a t e C r e a t e

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What’s Flight iGEM

Flight iGEM

iGEM Server

Create C r e a t e C r e a t e Edit Edit

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What’s Flight iGEM

Flight iGEM

iGEM Server

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What’s Flight iGEM

Flight iGEM

iGEM Server

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What’s Flight iGEM

Flight iGEM

iGEM Server

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What’s Flight iGEM

Flight iGEM

iGEM Server

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With Flight iGEM

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With Flight iGEM

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With Flight iGEM

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With Flight iGEM

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With Flight iGEM

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With Flight iGEM

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Improvement

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Improvement

Without Flight iGEM

!

  • !
  • !
  • Create templates

Prepare documents Upload images

Update images’ address

Upload code

Merge document into template

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Improvement

Without Flight iGEM With Flight iGEM

!

  • !
  • !
  • Create templates

Prepare documents Upload images

Update images’ address

Upload code

Merge document into template

!

  • !
  • !
  • Create templates

Prepare documents Upload images

Update images’ address

Upload code

Merge document into template

!

  • !
  • !
  • Create templates

Prepare documents Fill form Click

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Structure of Flight iGEM

Three engines, for:

  • 1. Content management: online

collaboration platform

  • 2. Template: page builder
  • 3. Carriage: page carrier
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Summary of Flight iGEM

  • PHP based & open-sourced platform
  • User-friendly interface
  • Automatic page builder
  • Online collaboration with version control
  • Saving time!!!
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Efficiency

Flight iGEM

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Efficiency

  • +

Days 20 40 60 80

Flight iGEM

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Efficiency

  • +

Days 20 40 60 80

75

Flight iGEM

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Efficiency

  • +

Days 20 40 60 80

10 75

Flight iGEM

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Present

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Present

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Present

Yes 94% No 6%

Will you use our platform?

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Achievements

  • We presented a Poster and a Talk.
  • We create our wiki with:
  • 1. an Attributions page;
  • 2. documented our standard BioBrick Parts and submitted them to

the iGEM Registry;

  • 3. documented parts;
  • Experimentally validate at least 4 new BioBrick Parts and submit

them to the iGEM Parts Registry.

  • We integrated the investigated issues into the design and execution
  • f our project for both Yogurt Guarder and the Flight iGEM.
  • Help 2 iGEM teams to build their wiki using the Flight iGEM.
  • Human Practices: visited a diary factory, conducted survey,

educated the public for proper yogurt storage.

  • iGEM Special Prize: we created a Software Tool to simplify iGEM

wiki building.

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Reference

  • 1. Ascenso OS, et al. (2011) An efficient synthesis of the precursor of AI-2, the signalling

molecule for inter-species quorum sensing. Bioorganic & medicinal chemistry 19(3):1236-1241.

  • 2. Bao Y, et al. (2013) Methylthioadenosine/S-adenosylhomocysteine nucleosidase (Pfs) of

Staphylococcus aureus is essential for the virulence independent of LuxS/AI-2 system. International journal of medical microbiology : IJMM 303(4):190-200.

  • 3. Blair WM & Doucette GJ (2013) The Vibrio harveyi bioassay used routinely to detect AI-2

quorum sensing inhibition is confounded by inconsistent normalization across marine

  • matrices. Journal of microbiological methods 92(3):250-252.
  • 4. Cappuyns AM, Bernaerts K, Vanderleyden J, & Van Impe JF (2009) A dynamic model for

diauxic growth, overflow metabolism, and AI-2-mediated cell-cell communication of Salmonella Typhimurium based on systems biology concepts. Biotechnology and bioengineering 102(1): 280-293.

  • 5. Galloway WR, Hodgkinson JT, Bowden SD, Welch M, & Spring DR (2011) Quorum sensing

in Gram-negative bacteria: small-molecule modulation of AHL and AI-2 quorum sensing

  • pathways. Chemical reviews 111(1):28-67.
  • 6. Gamby S, et al. (2012) Altering the communication networks of multispecies microbial

systems using a diverse toolbox of AI-2 analogues. ACS chemical biology 7(6):1023-1030.

  • 7. Ha JH, et al. (2013) Crystal structures of the LsrR proteins complexed with phospho-AI-2

and two signal-interrupting analogues reveal distinct mechanisms for ligand recognition. Journal of the American Chemical Society 135(41):15526-15535.

  • 8. Pereira CS, McAuley JR, Taga ME, Xavier KB, & Miller ST (2008) Sinorhizobium meliloti, a

bacterium lacking the autoinducer-2 (AI-2) synthase, responds to AI-2 supplied by other

  • bacteria. Molecular microbiology 70(5):1223-1235.
  • 9. Pereira CS, Thompson JA, & Xavier KB (2013) AI-2-mediated signalling in bacteria. FEMS

microbiology reviews 37(2):156-181.

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Acknowledgement

  • Prof. Guangchao Sui
  • Prof. Yuhua Li
  • Prof. Chunbo Teng
  • Prof. Jingying Wang

Lecturer: Shengnan Zhu Lecturer: Chen Li iGEM Teams: NUDT_CHINA, NJU-CHINA, Peking Northeast Forestry University Harbin Institute of Technology National Natural Science Foundation of China School of Forestry, Northeast Forestry University School of Science, Northeast Forestry University College of Life Sciences, Northeast Forestry University

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THANKS

Welcome you to visit http://www.flightigem.com

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Q&A

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NICE