Demonstrating BMP Effectiveness With Microbial Source Tracking & - - PowerPoint PPT Presentation

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Demonstrating BMP Effectiveness With Microbial Source Tracking & - - PowerPoint PPT Presentation

Nov 28, 2023 185 likes •387 views

Demonstrating BMP Effectiveness With Microbial Source Tracking & Host Fecal Score June 20th-21st, 2018 3rd Annual Conference on Environmental Conditions of the Animas and San Juan Watersheds Providing Genetic & Analytic Solutions for

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Demonstrating BMP Effectiveness With Microbial Source Tracking & Host Fecal Score

June 20th-21st, 2018 3rd Annual Conference on Environmental Conditions of the Animas and San Juan Watersheds

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Providing Genetic & Analytic Solutions for Water

Accredited* Water DNA Lab

*World’s only ISO 17025 Accredited MST Lab

Project & Site Analytics Pathogens (BSL2) Digital PCR Nutrient Source Tracking Host Fecal Score

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Increasing pressure to “get it right”

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Lesson from other industries

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How does genetic technology solve?

Challenges handling fecal pollution in water Microbial Source Tracking Where is the pollution coming from?

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Unclear who is responsible?

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How do I evaluate BMP effectiveness?

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Available Tools - Legacy Testing (Culture FIB)

Concerns: Ineffective at discriminating between sources.

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Available Tools - Field Observations

Concerns: Circumstantial and subjective evidence. Difficult to defend.

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Consequence

  • Hinders source abatement

○ Source identification must precede mitigation

  • Weakens the chain of inference

○ Not all sources present the same level of human health risk ■ Non-fecal < fecal ■ Non-human < human

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DNA-based Microbial Source Tracking

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Microbial Source Tracking - Maturity

Precedent

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Projects in >40 States

Credible Tests

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National Validation (SIPP)

Access to Technology

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Laboratories (Accredited)

Objective Interpretation

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Host Fecal Score

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Microbial Source Tracking is being applied at each stage of the CWA*

*(Source Molecular national projects from 2010-2017) Water Quality-Based Approach of the Clean Water Act

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MST’s in stormwater permitting

GA NPDES Industrial Storm Water General Permits

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Sampling and Testing Plan

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~$250-$800/sample

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Site Prioritization - Florida DEP/Martin County

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Where is the pollution coming from - Boston Water and Sewer

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  • First ever effectiveness assessment of MS4 IDDE program using DNA markers
  • Human markers measured at outfalls regardless of degree of IDDE completion, and

conventional tools (test kits) found to be insufficiently sensitive or specific for detecting illicit discharges

  • New IDDE procedures now recommended, including DNA markers to improve program

effectiveness

  • Outcome will be greater bacteria and phosphorus reduction (at lower unit cost and greater

health benefit than Green Infrastructure), moving City closer to TMDL compliance

  • Project recognized with national O&M Performance Award from NACWA
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Demonstrating BMP effectiveness - Santa Barbara

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Next Steps - Automation

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https://www.youtube.com/watch?v=i_2Q1DFLp3g

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Project Level Probabilistic Modeling

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Summary

Genetics is a superior tool for water quality monitoring It can be used for effective investigations of bacteria impairment Building a data driven approach based on rich DNA analytics A powerful tool for regulatory prioritization (QMRA/Natural Source Exclusions)

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Thank You

Mauricio Larenas CEO Linkedin mlarenas@sourcemolecular.com 786-220-0379

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www.sourcemolecular.com