DeepM Aligner, A new open source Aligner for NGS data Mert Ahmet - PowerPoint PPT Presentation

DeepM Aligner, A new open source Aligner for NGS data Mert Ahmet Kuşkucu , Kenan Midilli İstanbul University Cerrahpasa School of Medicine Department of Medical Microbiology Department of Virology

Data An Analysis i is The Bott ttle Ne Neck of of NG NGS

Deep M Aligner; • DeepM Aligner is a new open source software that uses Bowtie 2 and Burrows - Wheeler - Aligner (BWA) for NGS data interpretation . • We developed the software for analysis of HIV drug resistance .

The Algorithm: Indexing Indexing BT2 And DMA script BWA and DMA script Aligment of NGS Data Aligment (BT2) and DMA script (BWA) and DMA script Sorting of Data Samtools and DMA script Variant Calling ; VarScan and DMA script Generation of 1%, 10% and 20 % Generation of Covarage Graph Report Generation ; consensus files in fasta format GNU Plot and DMA script Stanford DB, Geno 2 Pheno etc . DMA script Linux OS, Perl

Bowtie2, BWA and DeepChek In < 20 % variant files have 100 % agreement Bowtie2 and BWA in <10% variant files have 100 % agreement In <1% variant files have minor differences; p distance mean is 0,0014, min 0,00157 , max 0,00315)

DeepM Aligner kuskucum@gmail.com Thanks for your kind attentions…