Cytokines Cytokines Design Result Design Result Oct4-11R - PowerPoint PPT Presentation

Cytokines

Cytokines

• Design • Result

• Design • Result

Oct4-11R

Oct4-11R

luxI luxR P luxI RNase Barnase

luxI luxR P luxI RNase Barnase

luxI luxR P luxI RNase Barnase

• Design • Result

Population-control circuit:

Detecting device in individual E. coli:

• Design • Modeling • Result • Highlight

• Design • Modeling • Result • Highlight

Non-chondrocytes

Chondrocytes

Chondrocytes

Chondrocytes

Signal Pathway

• Design • Modeling • Result • Highlight

Ca 2+ influx rate=5.0 Ca 2+ influx rate=2.0 Transcription activity Ca 2+ influx rate=0.9 Ca 2+ influx rate=0.6

• Design • Modeling • Result • Highlight

Chondrocytes Non-chondrocytes (ATDC5) (C3H101/2) Confocal Microscope (Leica)

Lane Transfection Illumination Number Time 1 - - 2 + - 3 + 20 min 4 + 30 min 1 2 3 4 zif268 GAPDH RT-PCR Real time PCR

* * Both MEF2 & CRE promoters initiate transcription

MEF2-JeT transcription activity increases slightly

CRE-JeT transcription activity increases dramatically

* * CRE-JeT promoter works well with the stimuli of Calcium

* Light induces promoter MEF2-JeT

hypertrophic chondrocytes dedifferentiation expressed in ATDC5 cell line

• Design • Modeling • Result • Highlight

Signal Pathway

Prokaryotic approach • Successfully tested hypoxia-inducible promoter • Developed model to predict the behavior of our bacteria Eukaryotic approach • Successfully constructed tissue-specific promoter • Realized eukaryotic light-control system • Expressed Oct4 in chondrocyte cell line Comparison • Eukaryotic: detect the signals more sensitively • Eukaryotic: target proteins function more directly • Prokaryotic: Easier and more convenient to control

Thanks, !