Cytokines Cytokines Design Result Design Result Oct4-11R - - PowerPoint PPT Presentation

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Cytokines Cytokines Design Result Design Result Oct4-11R - - PowerPoint PPT Presentation

Sep 18, 2022 483 likes •1.24k views

Cytokines Cytokines Design Result Design Result Oct4-11R Oct4-11R luxI luxR P luxI RNase Barnase luxI luxR P luxI RNase Barnase luxI luxR P luxI RNase Barnase Design Result Population-control circuit: Detecting

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Cytokines

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Cytokines

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  • Design
  • Result
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  • Design
  • Result
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Oct4-11R

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Oct4-11R

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luxI luxR PluxI RNase Barnase

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luxI luxR PluxI RNase Barnase

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luxI luxR PluxI RNase Barnase

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  • Design
  • Result
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Population-control circuit:

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Detecting device in individual E. coli:

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  • Design
  • Modeling
  • Result
  • Highlight
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  • Design
  • Modeling
  • Result
  • Highlight
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Non-chondrocytes

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Chondrocytes

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Chondrocytes

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Chondrocytes

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Signal Pathway

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  • Design
  • Modeling
  • Result
  • Highlight
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Ca2+ influx rate=0.6 Ca2+ influx rate=0.9 Ca2+ influx rate=2.0 Ca2+ influx rate=5.0

Transcription activity

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  • Design
  • Modeling
  • Result
  • Highlight
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Confocal Microscope (Leica)

Chondrocytes (ATDC5) Non-chondrocytes (C3H101/2)

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1 2 3 4

zif268

GAPDH

RT-PCR Real time PCR Lane Number Transfection Illumination Time 1

  • 2

+

  • 3

+ 20 min 4 + 30 min

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Both MEF2 & CRE promoters initiate transcription

* *

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MEF2-JeT transcription activity increases slightly

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CRE-JeT transcription activity increases dramatically

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CRE-JeT promoter works well with the stimuli of Calcium

* *

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Light induces promoter MEF2-JeT

*

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hypertrophic chondrocytes dedifferentiation expressed in ATDC5 cell line

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  • Design
  • Modeling
  • Result
  • Highlight
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Signal Pathway

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Prokaryotic approach

  • Successfully tested hypoxia-inducible promoter
  • Developed model to predict the behavior of our bacteria

Eukaryotic approach

  • Successfully constructed tissue-specific promoter
  • Realized eukaryotic light-control system
  • Expressed Oct4 in chondrocyte cell line

Comparison

  • Eukaryotic: detect the signals more sensitively
  • Eukaryotic: target proteins function more directly
  • Prokaryotic: Easier and more convenient to control
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Thanks,

!