An Introduction to Membrane Proteins October 19, 2010 Outline for - - PDF document
10/19/2010 An Introduction to Membrane Proteins October 19, 2010 Outline for class today What does a cell look like? Cell membranes Types of membrane proteins G-protein Coupled Receptors (GPCRs) Cell signaling cascade
Wikipedia.com
What molecules can move through the membrane?
– light, odorants, hormones, neurotransmitters neurotransmitters – Ex: adrenalin, nicotine, epinephrin, adenosine
– smell, taste, vision, neuronal function, blood pressure, heart rate
ATP cAMP
glucose production, cell cycle regulation
– GFP glows GREEN when illuminated with UV light – This protein is found in Aequorea victoria jelly fish. – 2008 Nobel prize in Chemistry went to researches who discovered GFP
Membrane dye (Red) Membrane dye (Red) inside GPCR-CFP (cyan)
PAFAH-II traffics to the intercellular membranes under stress, the myristoyl tail anchors the protein PAFAH-II can be cytoplasmic under normal cellular conditions
PAFAH-II cleaves damaged phospholipids Stress produces fragmented phospholipids in intracellular membranes damaged phospholipids, thereby protecting membranes
Oxidative stress Unstressed cells, YFP tagged PAFAH-II is primarily cytoplasmic Stressed cells, YFP tagged PAFAH-II is primarily membrane anchored
Unstressed cells Stressed cells