A chemical synapse, by EM - - PowerPoint PPT Presentation

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Apr 09, 2023 174 likes •303 views

A chemical synapse, by EM http://web.as.uky.edu/Biology/faculty/cooper/CooperRes2.htm An electrical synapse, by EM http://electroneubio.secyt.gov.ar/Bennett-neurons'electrical_synchronization.htm Purkinje neuron filled with fluorescent dye and

SLIDE 1

A chemical synapse, by EM

http://web.as.uky.edu/Biology/faculty/cooper/CooperRes2.htm

SLIDE 2

An electrical synapse, by EM

http://electroneubio.secyt.gov.ar/Bennett-neurons'electrical_synchronization.htm

SLIDE 3

Purkinje neuron filled with fluorescent dye and viewed with a light microscope

http://education.med.nyu.edu/courses/brainandbehavior/courseware/neurohist/html/0101.html

SLIDE 4

Drosophila larva with GFP expressed in a subset

f sensory neurons

http://www.nig.ac.jp/section/emoto/emoto-e.html

SLIDE 5

C. elegans with synapses labeled by GFP

http://thalamus.wustl.edu/nonetlab/ResearchF/elegans.html

Neuromuscular junctions labeled by tagging a synaptic protein with GFP

SLIDE 6

Simultaneous intracellular and extracellular recording!

Henze et al. (J Neurophyiol, 2000)

SLIDE 7

Patch-clamp recording

Neher (Sci Am, 1992)

SLIDE 8

“Semi-intact” preparation

Leech swimming circuit (Stent et al., Science, 1978)

SLIDE 9

Intracellular recording during fictive behavior

SLIDE 10

Paired recordings in locusts

Robertson (Trends Neurosci, 1996)

excitatory inhibitory

SLIDE 11

Combining genetics with electrophysiology

Antenna lobe projection neuron (Gouwens & Wilson, 2009)

SLIDE 12

GCaMP: A fluorescent indicator of neural activity via calcium levels

calcium-bound state calcium-free state

http://en.wikipedia.org/wiki/GCaMP