A Brief Tour of Research Core Facilities Genomics and Bioinformatics - - PowerPoint PPT Presentation

A Brief Tour of Research Core Facilities

Genomics and Bioinformatics

• Sequencing and Library Preparation: http://research.ncsu.edu/gsl
• Bioinformatics and Statistical Consulting: http://brc.ncsu.edu/consulting/
• High‐Performance Computing: http://www.ncsu.edu/itd/hpc/About/About.php

Structural Analysis

• NMR facilities: http://www.ncsu.edu/chemistry/nmr/

http://biochem.ncsu.edu/NMR/index.php

• X‐Ray Crystallography: http://www.xray.ncsu.edu/

http://biochem.ncsu.edu/XRC/

• Mass Spectrometry: http://www.ncsu.edu/chemistry/msf/index.html

http://biochem.ncsu.edu/MassSpec/index.php

Structural Analysis

• Scanning Microscopy Lab: http://www.aif.ncsu.edu/scanning‐microscopy‐laboratory/
• Surface Science Laboratory: http://www.aif.ncsu.edu/surface‐science‐lab/
• X‐Ray Diffraction Laboratory: http://www.aif.ncsu.edu/xrd‐laboratory/
• Nanofabrication Facility: http://www.nnf.ncsu.edu/

Structural Analysis

http://www.aif.ncsu.edu/ http://www.nnf.ncsu.edu/

Imaging Facilities

• Wide‐field and Confocal Imaging: http://www.ncsu.edu//project/cmif‐micro/
• Laser Imaging and

Vibrational Spectroscopy: http://www.ncsu.edu/chemistry/livs/index.html

• Electron Microscopy: http://www.ncsu.edu/cem/

http://www.aif.ncsu.edu/tem‐lab/

• Small and

Large Animal Imaging: http://www.ncstatevets.org/radiology/

Key Features

High Performance Field Emission Gun (X‐FEG) delivers high density and high quality electron beam Monochromator generates highly chromated electron source, significantly increases energy resolution from 0.8 eV to <0.2 eV Three‐Condenser Lens system makes parallel electron beam with diameter in Ångström scale Aberration Corrector removes the spherical aberration and other high order distortion, guarantees sub‐Ångström atomic resolution imaging Powerful EDS System (SUPER X): 4 SDD detectors around the sample, collect X‐Ray signal with higher solid angle, enhance the elemental mapping capability.  Typical elemental mapping takes several hours on conventional TEM, now can be finish in few minutes  Elemental mapping for some materials, such as nanoparticles, is impossible on conventional TEM, can be finished on Titan in few minutes;  Atomic resolution elemental mapping capability is unique, and new. High Resolution Electron Energy‐loss spectrum, <0.2 eV. More details about electron, bonding, band gap, valence information

FEI Titan ‐ Aberration Corrected S/TEM

Example in Si < 2 pm precision on atomic distances, with chemical sensitivity

FEI Titan ‐ Aberration Corrected S/TEM

RevSTEM (Patent Pending) Sang and LeBeau, Ultramicroscopy, March 2014

Cell and Tissue Analysis

• Flow Cytometry: https://cvm.ncsu.edu/research/labs/flow‐cytometry‐and‐cell‐

sorting‐laboratory/

• Histology: https://cvm.ncsu.edu/research/labs/population‐health‐

pathobiology/histology/

Animal Facilities and Clinical Trials

• Lab Animal Resources: https://cvm.ncsu.edu/c/l/lar/key_steps.html
• Biological Resources Facility: https://brf.sciences.ncsu.edu/
• Animal Procedures Laboratory: http://www.cvm.ncsu.edu/lar/cpl.html
• Gnotobiotic Core Facility: https://cvm.ncsu.edu/research/labs/population‐health‐

pathobiology/gastrointestinal‐biology/

• Animal Clinical Trials: https://cvm.ncsu.edu/research/centers/ccmtr/service‐cores/

Plant Growth and Genetics Facilities

• Growth Chambers: http://www.ncsu.edu/phytotron/
• Plant Transformation Lab: http://horticulture.cals.ncsu.edu/planttransformationlab/
• Plants for Human Health Institute: http://plantsforhumanhealth.ncsu.edu/
• Plant Recombining Service Center: http://www4.ncsu.edu/~jmalonso/Alonso‐

Stepanova_Home.html

• Plant Disease and Insect Clinic: http://www.cals.ncsu.edu/plantpath/extension/clinic/

UNC System Core Facilities

• UNC‐CH Core Facilities: http://www.med.unc.edu/corefacilities