A Brief Tour of Research Core Facilities Genomics and Bioinformatics - PowerPoint PPT Presentation

A Brief Tour of Research Core Facilities

Genomics and Bioinformatics Sequencing and Library Preparation: http://research.ncsu.edu/gsl • Bioinformatics and Statistical Consulting: http://brc.ncsu.edu/consulting/ • High ‐ Performance Computing: http://www.ncsu.edu/itd/hpc/About/About.php •

Structural Analysis NMR facilities: http://www.ncsu.edu/chemistry/nmr/ • http://biochem.ncsu.edu/NMR/index.php X ‐ Ray Crystallography: http://www.xray.ncsu.edu/ • http://biochem.ncsu.edu/XRC/ Mass Spectrometry: http://www.ncsu.edu/chemistry/msf/index.html • http://biochem.ncsu.edu/MassSpec/index.php

Structural Analysis Scanning Microscopy Lab: http://www.aif.ncsu.edu/scanning ‐ microscopy ‐ laboratory/ • Surface Science Laboratory: http://www.aif.ncsu.edu/surface ‐ science ‐ lab/ • X ‐ Ray Diffraction Laboratory: http://www.aif.ncsu.edu/xrd ‐ laboratory/ • Nanofabrication Facility: http://www.nnf.ncsu.edu/ •

Structural Analysis http://www.aif.ncsu.edu/ http://www.nnf.ncsu.edu/

Imaging Facilities Wide ‐ field and Confocal Imaging: http://www.ncsu.edu//project/cmif ‐ micro/ • Laser Imaging and • Vibrational Spectroscopy: http://www.ncsu.edu/chemistry/livs/index.html Electron Microscopy: http://www.ncsu.edu/cem/ • http://www.aif.ncsu.edu/tem ‐ lab/ Small and • Large Animal Imaging: http://www.ncstatevets.org/radiology/

FEI Titan ‐ Aberration Corrected S/TEM High Performance Field Emission Gun (X ‐ FEG) delivers high Key Features density and high quality electron beam Monochromator generates highly chromated electron source, significantly increases energy resolution from 0.8 eV to <0.2 eV Three ‐ Condenser Lens system makes parallel electron beam with diameter in Ångström scale Aberration Corrector removes the spherical aberration and other high order distortion, guarantees sub ‐ Ångström atomic resolution imaging Powerful EDS System (SUPER X): 4 SDD detectors around the sample, collect X ‐ Ray signal with higher solid angle, enhance the elemental mapping capability.  Typical elemental mapping takes several hours on conventional TEM, now can be finish in few minutes  Elemental mapping for some materials, such as nanoparticles, is impossible on conventional TEM, can be finished on Titan in few minutes;  Atomic resolution elemental mapping capability is unique, and new. High Resolution Electron Energy ‐ loss spectrum , <0.2 eV. More details about electron, bonding, band gap, valence information

FEI Titan ‐ Aberration Corrected S/TEM RevSTEM (Patent Pending) Example in Si < 2 pm precision on atomic distances, with chemical sensitivity Sang and LeBeau, Ultramicroscopy , March 2014

Cell and Tissue Analysis Flow Cytometry: https://cvm.ncsu.edu/research/labs/flow ‐ cytometry ‐ and ‐ cell ‐ • sorting ‐ laboratory/ Histology: https://cvm.ncsu.edu/research/labs/population ‐ health ‐ • pathobiology/histology/

Animal Facilities and Clinical Trials Lab Animal Resources: https://cvm.ncsu.edu/c/l/lar/key_steps.html • Biological Resources Facility: https://brf.sciences.ncsu.edu/ • Animal Procedures Laboratory: http://www.cvm.ncsu.edu/lar/cpl.html • Gnotobiotic Core Facility: https://cvm.ncsu.edu/research/labs/population ‐ health ‐ • pathobiology/gastrointestinal ‐ biology/ Animal Clinical Trials: https://cvm.ncsu.edu/research/centers/ccmtr/service ‐ cores/ •

Plant Growth and Genetics Facilities Growth Chambers: http://www.ncsu.edu/phytotron/ • Plant Transformation Lab: http://horticulture.cals.ncsu.edu/planttransformationlab/ • Plants for Human Health Institute: http://plantsforhumanhealth.ncsu.edu/ • Plant Recombining Service Center: http://www4.ncsu.edu/~jmalonso/Alonso ‐ • Stepanova_Home.html Plant Disease and Insect Clinic: http://www.cals.ncsu.edu/plantpath/extension/clinic/ •

UNC System Core Facilities UNC ‐ CH Core Facilities: http://www.med.unc.edu/corefacilities •