SLIDE 1 2019, Sublethal effects of clothianidin, a neonicotinoid insecticide that alters bee behavior and chlorantraniliprole, a bee friendly insecticide, on movement, pollen consumption, reproduction, and hive health
Erica Brock, CFANS RAP student, Temo Balaxashvilli MAST student, and Vera Krischik. Assoc Prof Entomology
SLIDE 2 Understanding the partial contribution
- f pesticides to bee mortality and
developing BMP to mitigate mortality, IPM is part of BMP
SLIDE 3
Neonicotinoid birds + bees: Fate of neonicotinoids + pathways of environmental contamination
(Sanchez-Bayo 2014 Science)
SLIDE 4 Why are neonicotinoids so much more toxic to bees compared to other insecticides?
- Receptors in bees not in mammals
- Adjuvants increase toxicity
- Alters behavior +foraging at sublethal doses
- Water soluable
- Binds with soil
SLIDE 5
2019, Sublethal effects of clothianidin, a neonicotinoid insecticide that alters bee behavior and chlorantraniliprole, a bee friendly insecticide, on movement, pollen consumption, reproduction, and hive health of the native, common bumblebee Bombus impatiens
SLIDE 6
2019, Sublethal effects of clothianidin, a neonicotinoid insecticide that alters bee behavior and chlorantraniliprole, a bee friendly insecticide, on movement, pollen consumption, reproduction, and hive health I participated in a study with bumble bees in the greenhouse in large tents. Bumbles were assigned to 3 treatments (n=8hives/trt); controls 0 ppb insecticide, clothianidin 20 ppb, and chlorantraniliprole 4ppm. Clothianidin provide in sugar syrup resulted in decreased movement, pollen consumption, colony weight, bee movement, and brood production. Chlorantraniliprole at 4ppm was no different than controls for all measurements of bee and colony health.
SLIDE 7
Measuring colony growth thru time
SLIDE 8
2018 field exp Bumble bees at 2 and 3 wks, clothianidin trts starts to decline at 2 wks, fungus
20 ppb clothianidin 4ppm chlorantraniliprole control
SLIDE 9 Bumblebee (8/trt) pollen consumption (g) over time clothianidin treatment eat less pollen over time
n= blue, clothianidin, class neonicotinoid, highly toxic to bees A= orange, chlorantraniliprole, a bee friendly insecticide c= grey, control
SLIDE 10 Bumblebee (8/trt) sugar syrup consumption (g) over time clothianidin treatment eat similar amounts over time
n= blue, clothianidin, class neonicotinoid, highly toxic to bees A= orange, chlorantraniliprole, a bee friendly insecticide c= grey, control
0.00 200.00 400.00 600.00 800.00 1000.00 1200.00 1400.00 2/19/2019 2/26/2019 3/5/2019 3/12/2019 3/15/2019 3/19/2019
Beehappy consumption
N A C
SLIDE 11 Bumblebee (8/trt) colony weight (g) over time clothianidin treatment has less weight over time
n= blue, clothianidin, class neonicotinoid, highly toxic to bees A= orange, chlorantraniliprole, a bee friendly insecticide c= grey, control
0.00 50.00 100.00 150.00 200.00 250.00 300.00 350.00 400.00 2/19/2019 2/22/2019 2/26/2019 3/1/2019 3/5/2019 3/8/2019 3/12/2019 3/15/2019 3/19/2019
Cumulative WT Change
N A C
SLIDE 12 Bumblebee (8/trt) colony movement over time clothianidin treatment has less movement over time
n= blue, clothianidin, class neonicotinoid, highly toxic to bees A= orange, chlorantraniliprole, a bee friendly insecticide c= grey, control
0.00 5.00 10.00 15.00 20.00 25.00 3/5/19 3/8/19 3/12/19 3/15/2019 3/19/2019
Movement
N A C
SLIDE 13 Bumble bee (8/trt) full honey pots over time all treatments not different over time
n= blue, clothianidin, class neonicotinoid, highly toxic to bees A= orange, chlorantraniliprole, a bee friendly insecticide c= grey, control
0.00 20.00 40.00 60.00 80.00 100.00 120.00 140.00 160.00 180.00 200.00 2/19/2019 2/22/2019 2/26/2019 3/1/2019 3/5/2019 3/8/2019 3/12/2019 3/15/2019 3/19/2019
Full honey pots
N A C
SLIDE 14 Bumble bee (8/trt) total brood over time clothianidin treatment has less total brood over time
n= blue, clothianidin, class neonicotinoid, highly oxic to bees A= chlorantraniliprole, a bee friendly insecticide c= control
0.00 20.00 40.00 60.00 80.00 100.00 120.00 2/19/2019 2/22/2019 2/26/2019 3/1/2019 3/5/2019 3/8/2019 3/12/2019 3/15/2019 3/19/2019
Total brood
N A C
SLIDE 15 Bumblebee (8/trt) brood stage 1 over time clothianidin treatment has less stage 1 young brood over time
n= blue, clothianidin, class neonicotinoid, highly toxic to bees A= orange, chlorantraniliprole, a bee friendly insecticide c= grey, control
0.00 10.00 20.00 30.00 40.00 50.00 60.00
Brood stage 1
N A C
SLIDE 16
Thanks to the CFANS RAP AP fellowship and the Krischik l lab for h r hosting m me to participate in bumbleb ebee ee resea earch
