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Brain Lungs Heart Liver Blood vessels Pancreas Kidney Cartilage Bone Muscle
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1 Scanning electron micrograph of Mycobacterium tuberculosis 2 3 - - PowerPoint PPT Presentation
1 Scanning electron micrograph of Mycobacterium tuberculosis 2 3 - - PowerPoint PPT Presentation
Brain Blood vessels Lungs Heart Pancreas Liver Kidney Cartilage Bone Muscle 1 Scanning electron micrograph of Mycobacterium tuberculosis 2 3 Sarah Stanley Deb Hung 4 1.7 million deaths/year Tuberculosis 9.2 million new
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Deb Hung Sarah Stanley
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Tuberculosis
Estimated new TB cases per 100,000 population 1.7 million deaths/year 9.2 million new cases/year WHO report, 2008
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Search for tuberculosis treatments
green = tuberculosis bacteria blue = human cells
With drug Without drug
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Add thousands of test chemicals, each chemical in a different well
Put bacteria and cells in individual wells of multi-well plates
Automated microscope
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Rifampicin - positive control
Number of bacteria : Number of human cells : Average roundness of human cells (choose a score = 10,9,8,7,6,5,4,3,2,1): 10 = round irregular = 1
15 211 5
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Computerized image analysis
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Find bacteria Count the number of bacteria per cell Find cells
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www.CellProfiler.org
Cell measurements
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Remaining slides go along with the teacher’s video guide
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www.CellProfiler.org
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www.Scratch.mit.edu
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