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Nov 24, 2023 9 likes •79 views

Yeasts associated with Drosophila in vineyard ecosystems Kate Howell Wine yeasts produce volatile compounds important for wine quality Grape juice/ must containing 200-300 g/L glucose + fructose Hanseniaspora S. cerevisiae Pichia spp.

SLIDE 1

Yeasts associated with Drosophila in vineyard ecosystems

Kate Howell

SLIDE 2

S. cerevisiae

Candida spp. Hanseniaspora spp.

Pichia spp.

Torulaspora spp.

Grape juice/ must containing 200-300 g/L glucose + fructose Ethanol +++ CO2 +++ Glycerol ++ Ethanol ++ CO2 + Glycerol +++ Acetic acid + Ethanol + CO2 + Glycerol + Acetaldehyde + Ethyl acetate +++ Ethanol ? CO2 ? Thiols +++ Ethanol ++ CO2 Succinic acid +

Wine yeasts produce volatile compounds important for wine quality

SLIDE 3

Drosophila flies choose a habitat based on volatiles

Yeast metabolism

– esters – higher alcohols – CO2 – ethanol

Yeasts are food
but, yeasts are vectored

Pham & Ray 2015 Scientific Reports

SLIDE 4

1. Do Drosophila species differ in

different parts of the vineyard and winery?

2. Are Drosophila associated

with yeasts, and are these species different in the vineyard and winery?

3. Are there any special

relationships between wine environment Drosophila and yeasts?

SLIDE 5

6,10% 92,68% 1,22%

S. lativittata

D.melanogaster and D.simulans D.hydei

0,47% 97,20% 2,34%

Drosophila species are differentially distributed in the vineyard ecosystem

Transform: Log(X+1) Resemblance: S17 Bray Curtis similarity

location

Vineyard Winery Marc 1 2 3 4 5 6 7 8 9 10 11 12

2D Stress: 0.05

Mount Langi n=214 Yering Station n=82

SLIDE 6

8,57% 62,86% 22,86% 5,71%

Mt. Langi Ghiran

H.uvarum H.valbyensis M.pulcherrima

P. membranifaciencs

n=82

35,54% 26,65% 24,68% 2,96% 9,87% 0,30%

Yering Station

H.uvarum H.valbyensis M.pulcherrima

P. membranifaciencs

T.delbrueckii

L. thermotolerans

n=214

And these Drosophila carry different yeasts