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the hidden half 30 March 2015 Tobias Wojciechowski, PhD Mitglied - PowerPoint PPT Presentation

Aug 31, 2022 •287 likes •657 views

Root phenotyping: uncovering the hidden half 30 March 2015 Tobias Wojciechowski, PhD Mitglied der Helmholtz-Gemeinschaft Soil conditions Mitglied der Helmholtz-Gemeinschaft Soil conditions What below-ground root architecture targets

  1. Root phenotyping: uncovering the ‘hidden half’ 30 March 2015 Tobias Wojciechowski, PhD

  2. Mitglied der Helmholtz-Gemeinschaft Soil conditions

  3. Mitglied der Helmholtz-Gemeinschaft Soil conditions

  4. What below-ground root architecture targets for breeding? Nutrient foraging Effective uptake of nutrients Metabolic efficiency Mitglied der Helmholtz-Gemeinschaft

  5. Root angle – steep vs shallow Mitglied der Helmholtz-Gemeinschaft Barley root system Rooting depth is correlated with root angle: shallow angles – shallow root system Maize root system

  6. Soil constrains in top- and subsoils • Plant roots encounter more constrains with depth and unequal distribution of nutrients • Development of ideotypes e.g. shallow roots for P-acquisition in top soil P Ca Shallow angles = K Water O 2 shallower roots N C° C° Hordeum vulgare Al salinity Mitglied der Helmholtz-Gemeinschaft Mn Steep angle = compaction deeper roots Zea mays

  7. Root architecture in 2- and 3-D Mitglied der Helmholtz-Gemeinschaft Postma, Schurr, Fiorani (2013)

  8. Mitglied der Helmholtz-Gemeinschaft 3-D imaging of roots

  9. Root carousel (IBG-2) Mitglied der Helmholtz-Gemeinschaft Nagel et al. (2009) Functional Plant Biology, 36: 947-959

  10. GROWSCREEN-RHIZO: a new automated system for 2D imaging of roots and shoots Mitglied der Helmholtz-Gemeinschaft Nagel et al. 2012

  11. Mitglied der Helmholtz-Gemeinschaft

  12. Visible root length correlates with global root parameters Mitglied der Helmholtz-Gemeinschaft Nagel et al. 2012

  13. Dynamic modeling of root architecture and N dynamics Mitglied der Helmholtz-Gemeinschaft

  14. Field phenotyping of roots • Integrates with technology at IBG-2 • Allows the screening of big plant numbers or populations (e.g. a barley diversity panel) Mitglied der Helmholtz-Gemeinschaft

  15. Field Phenotyping of roots ‘Shovelomics’ is a ‘low - tech’ high -throughput method, which allows to phenotype root crowns of single plants in great plant population of tropical and temperate grasses, legumes, and fobs Mitglied der Helmholtz-Gemeinschaft

  16. Manual Measurements nodal root Seminal root Mitglied der Helmholtz-Gemeinschaft Visual scoring: • Root classes (seminal and nodal roots) • Root numbers • Branching density • Root angles

  17. Mitglied der Helmholtz-Gemeinschaft Score board

  18. Mitglied der Helmholtz-Gemeinschaft Manual Measurements

  19. Mitglied der Helmholtz-Gemeinschaft Measurement

  20. Mitglied der Helmholtz-Gemeinschaft Complex root system

  21. Mitglied der Helmholtz-Gemeinschaft

  22. Mitglied der Helmholtz-Gemeinschaft

  23. Mitglied der Helmholtz-Gemeinschaft

  24. Mitglied der Helmholtz-Gemeinschaft

  25. Mitglied der Helmholtz-Gemeinschaft

  26. Mitglied der Helmholtz-Gemeinschaft

  27. Mitglied der Helmholtz-Gemeinschaft

  28. Data evaluation using DIRT software Mitglied der Helmholtz-Gemeinschaft

  29. Variation of anatomical root traits Nodal roots of barley plants Mitglied der Helmholtz-Gemeinschaft from a barley diversity panel

  30. Mitglied der Helmholtz-Gemeinschaft PSU Laser ablation technology by

  31. Mitglied der Helmholtz-Gemeinschaft Root traits Shoot traits

  32. Field proximal and remote sensing methods at IBG2 (presentation Francisco Pinto) Field-Lift Field-Mobile HyPlant Field-Bee Field-Ship Mitglied der Helmholtz-Gemeinschaft

  33. Gene / loci discovery research of Hannah Schneider (FZJ / PSU) Highly Expressed in Root Tissue Mitglied der Helmholtz-Gemeinschaft

  34. Soil constrains in deeper soils Plant roots encounter more constrains with depth and unequal distribution of nutrients P Ca K Water O 2 N Mitglied der Helmholtz-Gemeinschaft C° C° Al salinity Mn compaction

  35. Pre-breeding example (root traits) 1987- 20 years (with several seasons/year) of screening elite lines for P efficiency failed to beat Carioca Mitglied der Helmholtz-Gemeinschaft

  36. Mitglied der Helmholtz-Gemeinschaft Carioca

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