The Effects of Light Wavelengths (nm) on Isopod Habitat Choice - - PowerPoint PPT Presentation
The Effects of Light Wavelengths (nm) on Isopod Habitat Choice Background Isopods dwell in dark, moist environments As seen in lab, theyre found in leaf litter Of the crustacea class (terrestrial) cannot survive in water
❖ Isopods dwell in dark, moist environments ❖ As seen in lab, they’re found in leaf litter ❖ Of the crustacea class (terrestrial) → cannot survive in water ❖ They may also perform tonic immobility as a defence mechanism ➢ A state of reversible physical immobility or muscle hypertonicity (Quadros).
❖ Green, Red, and Full-Spectrum Lights were set up over chambers (in lab room with fluorescent lights on) ❖ 5 new isopods were collected for each trial ❖ 2 minutes were provided for acclamation ❖ Opened gates and let them roam for 5 minutes ❖ Gates closed and isopod location was recorded
❖ The experiment was conducted in a dark room to avoid outside light interference ❖ Green, Red, and Full-Spectrum Lights were set up over chambers in a dark room ❖ 5 new isopods were collected for each trial from the same environment ❖ 2 minutes were provided for acclamation ❖ Opened gates and let them roam for 5 minutes ❖ Gates closed and isopod location was recorded
Full Spectrum 400-700 nm Red 571-687 nm Green 495-571 nm Mean 4.67 2 1 SD 1.32951 1.732051 * Data, graph, test,and conclusion from the second week only
Figure 1: The number of isopods found in each choice chamber after being exposed to different wavelengths (nm) of light. The bars represent the mean; error bars represent the standard deviation. For the 400-700 nm wavelength 4.67 nm +/- 1.32951 nm, for the 571-687 nm wavelength 2 nm +/- 1.732051 nm, and for the 495-571 nm (1) +/- 1 nm.
Full Spectrum (400-700 nm) v. Red (571-687 nm) P = 0.078927582 > .05 Full Spectrum (400-700 nm) v. Green (495-571 nm) P = 0.015268072 < .05 Full Spectrum (400-700 nm) v. Full Spectrum (400-700 nm) P = 0.25 > .05 Red (571-687 nm) v. Green (495-571 nm) P = 0.422649731 > .05 *T test was used
➢ Indifferent to the red light ➢ Did not tend to gather under the green light when paired with the full spectrum light. Future Recommendations ➢ *Add damp leaf litter to see if wavelength still affects isopods’ habitat choice ➢ Try blue light instead of red Possible Sources of Errors ➢ Doing experiment with other light besides the ones used directly over the chambers ➢ The temperature of the dark room (68 degrees fahrenheit)
*Light Bulbs used