Th The Impacts of Temperature on I t f T t Wentletraps and - PowerPoint PPT Presentation

Th The Impacts of Temperature on I t f T t Wentletraps and Anemones e t et aps a d e o es Angelica Trujillo SBCC SBCC Biological Sciences Mentor: Stephen Gosnell Advisor: Steve Gaines Anji Trujillo 6/30/2010

Impacts of Interactions on Communities • Species interaction influence community influence community dynamics • Species interactions change with the change with the environment • Intertidal as Model Stephen Gosnell 11/19/2009 S System t

Research Focus How will the interaction between sea anemones and a wentletraps change with temperature? Goals • Locate field specimen L t fi ld i and determine abundance abundance • Determine impact of • Determine impact of Anthopleura sp Anthopleura sp. temperature on species Epitonium tinctum p and interaction and interaction 3 Anji Trujillo 7/3/10

Experimental Methods in the Field Experimental Methods in the Field • Collecting of specimens • Field Experiments • Developing search • Developing search protocol • Surveys S • Determine wentletrap population size and density • Mark and recapture • Mark and recapture 4

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Experimental Methods in the Lab Factorial Experiment • Explore the combined impacts of temperature food Explore the combined impacts of temperature, food availability, and predation on anemones. 5

Factorial Table Factorial Table Multiple Stressors Temperature Presence of predator F Food availability d il bilit Food/No Food Low Temp High Temp 12˚C 20˚C 12˚C, 20˚C, Predator Predator Predator 12˚C, 20˚C, No Predator No Predator No Predator 6

Assessing the Impact on Anemones on Anemones • Morphological measures p g • mass, color, death rate • Molecular methods • protein degradation Assessing the Impact on Assessing the Impact on Wentletraps • Acute temperature response • Acute temperature response 7

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Ubiquitun Dot Blot Standard Quantifying protein y g p degradation • Extract protein from sea -W,-T, +F anemone tentacles • Expose protein to anti-body Results : Not clear 12

Heat Block Experiment Heat Block Experiment Acute Temperature p Response • 90 minutes exposure from 12.1˚C - 31.5˚C R Result: all survived lt ll i d •Local water temp. does not exceed 20˚C 13

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Project Summary Project Summary Establish the impacts of temperature on wentletrap and sea anemone • Offer insight community dynamics • Similar anthozoan- gastropods relationships t d l ti hi found in coral reefs around the world. 11

Future Plans • Finish a second trial of the factorial experiment • Reassess the impact p • Go out into the field and re-evaluate population size and densities. population size and densities. • Take a look at larger number of protein extraction samples extraction samples • Assess wentletrap movement and feeding preferences f

Acknowledgments INSET staff INSET staff Mentor: Stephen Gosnell p Elizabeth Hoaglund Friends and Family 17