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Céline Jouanin – Anthony Vollant – Amélie Briane - Julien Cescut
2017-10-26 Rencontre IngéStats
Version 2017-10-23
TECHNOLOGY PLATFORMS OF TWB
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TECHNOLOGY PLATFORMS OF TWB Strain Biotransformation Process - - PowerPoint PPT Presentation
TECHNOLOGY PLATFORMS OF TWB Strain Biotransformation Process - - PowerPoint PPT Presentation
www.toulouse-white-biotechnology.com Cline Jouanin Anthony Vollant Amlie Briane - Julien Cescut Version 2017-10-23 2017-10-26 Rencontre IngStats TECHNOLOGY PLATFORMS OF TWB Strain Biotransformation Process engineering &
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BIOTRANSFORMATION FROM 50 mL TO 200L
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50 mL 500 mL 2 L 20 L 200 L
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THE NEED TO SCREEN
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Medium optimization Environmental parameter optimization Process optimization Process robustness optimization Industrial approved Time consuming 10n combinaisons bottleneck Strain evaluation Up & down process optimization HTS
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THE SOLUTIONS
Miniaturisation
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THE SOLUTIONS
Parallelization
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THE SOLUTIONS
Automatisation
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CUSTOM ROBOTIC PLATEFORM
X X
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ROBOTIC MOVEMENT PROGRAMMING
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MAIN WORKFLOW
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Preculture Conditionnal seeding Culture process Sampling Sample processing
I II III IV
1-50
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I PRECULTURE PHASE
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Incubation in plate reader (Xenius, Safas) monitoring of optical density
Preculture
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II SEEDING PHASE
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Communication between reader and liquid handling robot Conditionnal seeding
Conditional seeding
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III CULTURE
Overview of numerous signals regulator adaptation to the biological kinetics
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Culture process
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IV SAMPLING
Sampling
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SAMPLE PROCESSING
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total sample Filtration; centrifugation Cytometry, CDW, biobank HPLC, GC-MS
- 20 °C to + 4 °C
Sample processing
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RAW DATA
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MULTIFACTOR OPTIMIZATION
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Optimum :
- 37.4 °C
- 7.1 upH
Validation in 20 L (not shown) Cartography
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Glucose Acetic Acid µmax DO
Statistics exploitation of DoE with R, JMP
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DATA PROCESSING
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Acquire data Reformat and clean data Preparation Execute scripts Edit analysis scripts Debug Inspect
- utputs
Analysis - Automatisation Filtering Algorithm Vizualization Explore alternatives Report Archive experiment in database Make comparison New idea Meeting Reflection Share experiment Distribution&Storage Quality indicators New project New equipment New Analysis
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CONCLUSION
- Custom design of fermentation robotic plateform
- 24 instrumented mini-reactors
- Automatisation steps from preculture to supernatant
separation
- Acceleration of best strain or optimal environmental
parameters finding
- In progress: implementation of automatic data/file
processing and database setting up
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