[PPT] - Strategies for valorisation of wastes from bioethanol production PowerPoint Presentation

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CYPRUS 2016 4th International Conference on Sustainable Solid Waste Management

Strategies for valorisation of wastes from bioethanol production – lactic acid and probiotics as added value products

A. Djukić-Vuković1*, D. Mladenović1, J. Pejin2, L. Mojović1

1Faculty of Technology and Metallurgy, University of Belgrade 2Faculty of Technology, University of Novi Sad

*adjukic@tmf.bg.ac.rs

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Strategies for valorisation of wastes from bioethanol production

4th International Conference on Sustainable Solid Waste Management, Limassol , Cyprus, 23-25 June 2016

Competitiveness of bioethanol as a

alternative fuel is dependent on the environmentally friendly and economically feasible management of residues remaining after production process to 0.5% - advanced biofuels

is target - EU Indirect land use change directive (April 2015)

What about advanced bioethanol –

bioethanol produced on wastes and residues ? Stillage is a by‐product of bioethanol production on different substrates (corn, triticale, waste bread...).

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Strategies for valorisation of wastes from bioethanol production

4th International Conference on Sustainable Solid Waste Management, Limassol , Cyprus, 23-25 June 2016

Why lactic acid? Lactic acid (2-hydroxypropanoic acid) is widely used chemical in food, pharmaceutical and chemical industry – 7% annual growth rate. High demand for lactic acid is driven by increased production of polylactides (PLA, PLGA) - biocompatible polymers used for medical, pharmaceutical and food industry purposes. Specially attractive – LA for PLA produced from renewable sources.

Fermentation enables selective production of L or D isomer. WHO: Probiotics are microorganism with beneficial effect on health of host – human or animal → 10

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4th International Conference on Sustainable Solid Waste Management, Limassol , Cyprus, 23-25 June 2016

Strategies for valorisation of wastes from bioethanol production

Aim: Lactic acid production on thin stillage

Microorganism Lactobacillus rhamnosus ATCC 7469 – homofermentative, > 97% L(+)-lactic acid Fermentation media sterilized liquid wasted bread, liquid molasses stillage and liquid corn stillage from bioethanol plant Reahem and Alpis, Serbia, initial sugar concentration was set at approximately 25 g/L Fermentation Batch, shaking of 90 rpm, 5% (v/v) inoculum concentration 41°C, microaerophylic, pH control: without and with addition of neutralizing agents (CaCO3, 30% NaOH in 4h intervals)

Thin corn stillage Thin molasses stillage Thin wasted bread stillage Reducing carbohydrates (g L-1) 13.12 ± 0.70 4.20 ± 0.19 11.66 ± 0.68 Crude protein (g L-1) 8.42 ± 0.71 18.80 ± 0.61 21.00 ± 1.10 Ash (g L-1) 2.90 ± 0.32 17.60 ± 0.72 6.96 ± 0.23 Lipids (g L-1) 1.83 ± 0.45 0.80 ± 0.02 5.48 ± 0.81 Dry matter (%) 5.02 ± 0.29 6.61 ± 0.55 4.80 ± 0.48 Table 1. Chemical composition of different stillages

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Strategies for valorisation of wastes from bioethanol production

4th International Conference on Sustainable Solid Waste Management, Limassol , Cyprus, 23-25 June 2016

5 10 15

12 24 36 5 10 15 20 25

Lactic acid concentration (g L

1)

Time (h)

thin wasted bread stillage thin molassed stillage thin corn stillage

12 24 36 7.0 7.5 8.0 8.5 9.0 9.5 10.0

log N (CFU mL-1) Time (h)

The highest LA concentration - thin wasted bread stillage

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Strategies for valorisation of wastes from bioethanol production

4th International Conference on Sustainable Solid Waste Management, Limassol , Cyprus, 23-25 June 2016

LA concentration (g L-1) LA yield (g g-1) LA yield coefficient (g g-1) LA productivity (g L-1 h-1) Thin corn stillage 13.24 ± 0.20 0.55 ± 0.02 0.89 ± 0.03 0.44 ± 0.02 Thin molasses stillage 13.41 ± 0.47 0.60 ± 0.03 0.98 ± 0.05 0.45 ± 0.02 Thin wasted bread stillage 15.38 ± 0.27 0.69 ± 0.02 0.98 ± 0.03 0.51 ± 0.02

Table 2. LA fermentation parameters

CaCO3

1% CaCO3 2% CaCO3 5% CaCO3 10% CaCO3 2 4 6 8 10 12 14 16 18

Lactic acid concentration (g L

1)

5 6 7 8 9 10

LogN (CFU mL

1)

biomass lactic acid

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Strategies for valorisation of wastes from bioethanol production

4th International Conference on Sustainable Solid Waste Management, Limassol , Cyprus, 23-25 June 2016

2 4 6 8 10 12 14 16 18 20 12 24 36 48 60

Lactic acid concentration (g L

1)

Time (h)

CaCO3 NaOH

Thin wasted bread stillage
NaOH in 4h intervals

LA yield → 0.77 g g-1 LA productivity → 1.14 g L-1 h-1 Biomass → 8×109 CFU mL-1

L. rhamnosus ATCC 7469 has

the probiotic characteristics!

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WHOLE STILLAGE – HIGH GRAVITY STILLAGE (HGS) FEED (DDG – DRIED DESTILLER’S GRAINS ) DRYING PROBIOTIC- ZEOLITE ADDITIVE FOR FEED Solid fraction - L. rhamnosus immobilized

nto zeolite

Process II FEED WITH PROBIOTIC BIOMASS LA fermentation of HGS with free L. rhamnosus SEPARATION Solid fraction of fermentation media with L. rhamnosus ATCC 7469 biomass Liquid fraction of fermentation media with LA Process I THIN STILLAGE (TS) Lactic acid extraction SEPARATION SOLID PART OF STILLAGE SEPARATION LA fermentation of TS with immobilized L. rhamnosus Lactic acid extraction Liquid fraction of fermentation media with LA

Strategies for valorisation of wastes from bioethanol production

4th International Conference on Sustainable Solid Waste Management, Limassol , Cyprus, 23-25 June 2016

LACTIC ACID FERMENTATION

Proteins, 40.24% Lipids; 4,92% Ash; 0,35% Nitrogen-free extract, 49.35% Hemicellulose 4.01% Crude fibres; 2,04%

Feed for monogastric animals

PROBIOTICS

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Other possibilities ...

Strategies for valorisation of wastes from bioethanol production

4th International Conference on Sustainable Solid Waste Management, Limassol , Cyprus, 23-25 June 2016 WHOLE STILLAGE – HIGH GRAVITY STILLAGE (HGS) FEED (DDG – DRIED DESTILLER’S GRAINS ) DRYING PROBIOTIC- ZEOLITE ADDITIVE FOR FEED Solid fraction - L. rhamnosus immobilized

nto zeolite

Process II FEED WITH PROBIOTIC BIOMASS LA fermentation of HGS with free L. rhamnosus SEPARATION Solid fraction of fermentation media with L. rhamnosus ATCC 7469 biomass Liquid fraction of fermentation media with LA Process I THIN STILLAGE (TS) Lactic acid extraction SEPARATION SOLID PART OF STILLAGE SEPARATION LA fermentation of TS with immobilized L. rhamnosus Lactic acid extraction Liquid fraction of fermentation media with LA

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Conclusions

Strategies for valorisation of wastes from bioethanol production

4th International Conference on Sustainable Solid Waste Management, Limassol , Cyprus, 23-25 June 2016

Stillages from advanced bioethanol production have shown same or better

characteristics than stillage from 1st generation bioethanol as substrates for LAF.

Thin wasted bread stillage with pH control (NaOH in 4h intervals) -process

productivity was improved twofold.

Added value products:
Probiotic biomass >109 CFU mL-1
Animal feed – monogastric animals

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Thank you for attention!

Faculty of Technology and Metallurgy, University of Belgrade

adjukic@tmf.bg.ac.rs

Acknowledgem ent

This work was funded by Ministry of education, science and technological development, Republic of Serbia, Project number TR 31017

Strategies for valorisation of wastes from bioethanol production

4th International Conference on Sustainable Solid Waste Management, Limassol , Cyprus, 23-25 June 2016