Skin Measurement Methodology Development Kukizo Miyamoto Ph.D. - - PowerPoint PPT Presentation

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Skin Measurement Methodology Development Kukizo Miyamoto Ph.D. - - PowerPoint PPT Presentation

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Procter & Gamble Confidential New Breakthrough in Cosmetic: Skin Measurement Methodology Development Kukizo Miyamoto Ph.D. Principal Scientist P&G Innovation GK R&D Kobe Innovation Center, Japan Procter & Gamble Confidential

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New Breakthrough in Cosmetic: Skin Measurement Methodology Development

Kukizo Miyamoto Ph.D. Principal Scientist P&G Innovation GK R&D Kobe Innovation Center, Japan

Procter & Gamble Confidential

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Introduction – Importance of Skin Meas. Methods in Cosmetology

  • Cosmetic consumer seeks skin care products meeting

their concerns/QOL.

  • Our goal is to keep the best skin condition for longer

term with the cosmetic product and service.

  • To do this, solid but unique skin measurement methods

are required to understand the skin deeply and demonstrate product benefits.

  • We have developed novel but unique ‘in-vivo’ skin meas.

methods to accurately assess skin conditions, and detect early sign/symptom even before consumer notice, for superior cosmetic product development and skin research.

Procter & Gamble Confidential

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Background – Skin Meas. Methods in Cosmetology

  • Can we describe her skin?

Procter & Gamble Confidential

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Background – Skin Meas. Methods in Cosmetology

  • Can we describe much easier when compare?

T

Procter & Gamble Confidential

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Background – Skin Meas. Methods in Cosmetology

  • Can we describe much easier when compare?

T

Wrinkles/Fine Lines Tone evenness/Spots Texture/Pores Firmness/Radiance, etc

Procter & Gamble Confidential

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Skin Meas. Methods – Facial Imaging System

  • How we measure the skin condition ‘what we can see’?
  • Variety of image analysis algorithms were developed

Wrinkles/Fine Lines Tone evenness/Spots Texture/Pores Firmness/Radiance, etc

Wrinkle

Facial Imaging System (P&G Original, controlled lighting, camera, reposition, etc)

Procter & Gamble Confidential

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Skin Meas. Methods – Facial Imaging System

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0.0078 Wrinkles/Fine Lines 0.0617 0.014 Spots 0.071 0.017 Texture/Pores 0.047 L:60.7 Skin Lightness L:58.6 Procter & Gamble Confidential

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AKITA 10YR STUDY

Objectives 1.Holistic skin aging measure how skin ages across 10 years (track 100+ women in Akita, Japan) 2.Measure the impact of using Cosmetic Skin Care Regimen for 1 Year 1st measurement 1999 2nd measurement 2010

(11 yrs later)

3rd measurement 2011

(1 yr of Hero Regimen Use)

Procter & Gamble Confidential

Kukizo Miyamoto, et. al: Characterization of comprehensive appearance of skin aging: Journal of Dermatological Skin Science; 64, (2011) 229-236

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AKITA 10YR STUDY

Development of Wrinkles and Hyperpigmented Spots in 11 years

  • - As expected, hyperpigmented spots and wrinkles increased with age
  • - Hyperpigmented spots visible with UV light in 1999 became visible spots in 2010

Spots Wrinkles

Procter & Gamble Confidential

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Successful vs. Unsuccessful Skin Aging

Procter & Gamble Confidential

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  • Skin aging does not happen equally to everybody.
  • Some keep ideal skin over 10yrs.

AKITA 10YR STUDY- FINDINGS

Procter & Gamble Confidential

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AKITA 10YR STUDY- OUTCOME

Ideal Skin Correlation Skin Parameter 1 Firmness 2 Radiance 3 Spots 4 Wrinkle 5 Texture 6 Age 7 Skin Lightness 8 Skin Redness 9 Pores 10 Skin Yellowness

  • Identified the top 5 Dimensions (skin parameters) to

contribute good skin condition over years.

Procter & Gamble Confidential

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AKITA 10YR STUDY

Objectives

  • 1. Holistic skin aging measure how skin ages across 10 years (track 100+

women in Akita, Japan)

  • 2. Measure the impact of using Cosmetic Skin Care Regimen for 1 Year

1st measurement 1999 2nd measurement 2010

(11 yrs later)

3rd measurement 2011

(1 yr of Hero Regimen Use)

Procter & Gamble Confidential

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  • 1 year use of Skin Care Regimen delivers amazing skin

improvements - almost 10 years back!

  • Hard to see differences between 1999 vs. 2011 visuals.

AKITA 10YR STUDY- FINDINGS

Facial Treatment Essence Whitening Skin care Serum Aging Care Skin Moisturizer

Procter & Gamble Confidential

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Age 50 in 1999 Age 61 in 2010 Age 62 in 2011 1yr After Skin Care Regimen

Akita 10yr Study Baseline 10yr After  1yr After Skin Care Regimen

Procter & Gamble Confidential

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Skin Meas. Methods – Visible Skin Firmness

  • We even developed new meas. to represent ‘Cosmetic

Consumer Word’

  • Example – Skin Firmness Look…Can we measure it??

T

Procter & Gamble Confidential

Kukizo Miyamoto, et. al: Development of new in-vivo imaging of facial skin firmness: Skin Research & Technology , 19 (1) , 2012, pp. 525-531

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Skin Meas. Methods – Visible Skin Firmness

  • Example – Skin Firmness Look…Can we measure it??
  • They look at facial lines and its direction…!!!

Procter & Gamble Confidential

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Skin Meas. Methods – Visible Skin Firmness

  • Skin Firmness Look Measure…Ageless Vector!
  • Measure Facial Line + Direction

Procter & Gamble Confidential

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Skin Meas. Methods – Visible Skin Firmness

  • Skin Firmness Look Measure…Ageless Vector!
  • Measure ‘Angle’ of facial lines

Mean Direction of Facial Lines

Ageless Vector (Ө)

Total Area of Facial Lines

Ageless Vector (Ө)

Ageless Vector = 22.5 Ageless Vector = 49.3

Procter & Gamble Confidential

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Skin Meas. Methods – Visible Skin Firmness

  • Skin Firmness Look Measure = Ageless Vector (Aging Angle)
  • About 1 degree down by 1 yr.

Mean Direction of Facial Lines

Ageless Vector (Ө)

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  • tal

Area of Facial Lines

How’s your firmness look? = What is your Ageless Vector?

Procter & Gamble Confidential

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Skin Meas. Methods – Visible Skin Firmness

  • Cosmetic Efficacy Demonstration
  • Better Skin Firmness in 2 weeks – SK-II Skin Care Cream

Procter & Gamble Confidential

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Skin Meas. Methods – Invisible Spot Imaging

  • Melanin and Hemoglobin as 2 Key Skin Chromophores
  • Extract Only Melanin Information by Image Analysis Technique

R G B Melanin Thickness Hemoglobin Skin Spectra Reflectance

Infrared light

Epidermis

Papillary dermis Reticular dermis Stratum corneum incident light

S.C. & Epidermal back scattering Deep skin back scattering

Hemoglobin,

collagen Collagen Melanin, proteins, lipids Chromophores/ absorbers

B B G+ G+R R Infrared light

Epidermis

Papillary dermis Reticular dermis Stratum corneum incident light

S.C. & Epidermal back scattering Deep skin back scattering

Hemoglobin,

collagen Collagen Melanin, proteins, lipids Chromophores/ absorbers

B B G+ G+R R Skin Color

 SIA Scope Melanin image Measure mean. Brightness = Melanin level Hemoglobin image Measure mean. Brightness = Hemoglobin level Collagen image Measure mean. Brightness = Collagen level

Melanin Mapping Hemoglobin Mapping

Procter & Gamble Confidential

Paul Matts, et. al: The distribution of melanin in skin determined in vivo: British Journal of Dermatology , 156 (4), pp 620-628

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Original Image Hemoglobin Image

Good Purification of Hemoglobin oriented features such as lips and around eyes Procter & Gamble Confidential

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Original Image Melanin Image

Good Purification of Melanin oriented features such as hyperpigmented spots Procter & Gamble Confidential

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Hemoglobin Image Melanin Image

Good Purification of Melanin oriented features such as hyperpigmented spots Both Melanin and Hemoglobin in this mole Procter & Gamble Confidential

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Original Image After 8 Wks Image

You could see the difference in two images….

Procter & Gamble Confidential

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Original Image After 8 Wks Image

Now we can see more clearly with this technique!!

Procter & Gamble Confidential

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Skin Meas. Method – New Visualization of Skin Elasticity

Standard model (ED = 50 kPa) Aging Large gap (tipping edge)

Stage II Stage I

Epidermis elastic modulus Epidermis thickness

  • Mechanical Modeling for Elasticity & Thickness Change in

Skin Epidermis

  • There may be a drastic change in skin aging  Skin

Tipping Point!

  • Elasticity is important function of the skin
  • Can we measure early stage of skin elasticity damage, before

wrinkling or sagging??

Procter & Gamble Confidential

Kuwazuru O. Miyamoto K.: Skin wrinkling morphology changes suddenly in the early 30s, Skin Research and Technology 18 (4) 2012 , pp. 495-503

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Skin Meas. Method – New Visualization of Skin Elasticity

  • Skin Compression Imaging System – Meas. Deformation of Skin

0mm 2mm 3mm 4mm 5mm 6mm

Procter & Gamble Confidential

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Baseline

Sub0006 Age 31 Sub0020 Age 39

Procter & Gamble Confidential

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1mm

Procter & Gamble Confidential

Sub0006 Age 31 Sub0020 Age 39

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2mm

Procter & Gamble Confidential

Sub0006 Age 31 Sub0020 Age 39

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3mm

Procter & Gamble Confidential

Sub0006 Age 31 Sub0020 Age 39

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4mm

Procter & Gamble Confidential

Sub0006 Age 31 Sub0020 Age 39

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5mm

Procter & Gamble Confidential

Sub0006 Age 31 Sub0020 Age 39

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Sub0006 Age 31

Sub0020 Age 39

Baseline + Physical Stress

Skin Meas. Method – New Visualization of Skin Elasticity

Before Tipping After Tipping

Baseline + Physical Stress

Procter & Gamble Confidential

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Skin Meas. Method – New Visualization of Skin Elasticity

 Skin condition was drastically changed in age 30’s and Skin tipping point was calculated at age 35.09 from this study.  Skin condition varies significantly more after age 40.  Skin Tipping is critical moment may lead successful and unsuccessful skin aging.

Tipping Point

Age 35.1 Procter & Gamble Confidential

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In-Depth Skin Visualization/Method – Two Photon Tomography

Review article:

  • K. König (2008): Clinical multiphoton tomography.J. Biophotonics 1,13–23
15 µm 15 µm µ 3 I II III IV 15 µm 15 µm µ 3 I II III IV

I Epidermis A Stratum corneum B Stratum granulosum C Stratum spinosum D Stratum basale II-IV Dermis II Corium: Stratum papillare III Corium: Stratum reticulare IV Corium: Subcutaneous layer

5µm 100µm 80µm 30µm 15µm 50µm

Two photon absorption phenomenon only occurs at the excitation light focal point, which allows high-resolution by-layer in depth imaging/visualization.

Procter & Gamble Confidential

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In-Depth Skin Visualization/Method – Two Photon Tomography

  • Two Photon Technique – A new in-vivo imaging tool for in-depth profiling
  • NAD(P)H as key keratinocyte cell-activity biomarker can be directly visualized by a new

In-vivo two-photon imaging system available

SC layer Granular layer Spinosum layer Basal layer

Depth

  • Exclusive to visualize Granular layer cells
  • Size/distribution of keratinocyte by depth can be observed
  • Less information of SC layers

Inner forearm skin 2um depth step

Procter & Gamble Confidential

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Visualizing skin energy with Two Photon Tomography: NAD(P)H in Granular Cells

Procter & Gamble Confidential

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Two Photon Tomography – Age Dependency Study Results

Study Topline Results:

There is a significant decline in NAD(P)H levels by age from 20’s to 60’s (r=0.324, p<0.01) on the facial skin (n=86, age 21-68).

Procter & Gamble Confidential

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Facial Image – Age 20’s (Sub#0043, age=23) Facial Image – Age 60’s (Sub#0047, age=67)

Two Photon Tomography – Age Dependency Study Results

Procter & Gamble Confidential

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Two Photon Image – Age 20’s (Sub#0043, age=23) Two Photon Image – Age 60’s (Sub#0047, age=67)

Two Photon Tomography – Skin Energy (NAD(P)H) Age Dependency Study Results

Procter & Gamble Confidential

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SK-II Anti-Aging Lighthouse SK-II Anti-Aging Lighthouse

Visible Firmness 3D Melanin Spot

Magic Ring

Holistic Skin Condition Analysis

Finally – Magic Ring A Skin Counseling Tool Inducing Skin Meas. Technologies

Procter & Gamble Confidential

Kukizo Miyamoto, et. al: Development of rapid in-vivo imaging of facial skin appearances: Skin Research & Technology , 19 (1) , 2012, pp. 525-531

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