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Background and Theory Site Details Bacteria Lab Processing Lab Processing Analysis Conclusion
Prepared by Becky Jimenez NSF REU Civil Infrastructure Engineering, - - PowerPoint PPT Presentation
Prepared by Becky Jimenez NSF REU Civil Infrastructure Engineering, - - PowerPoint PPT Presentation
Prepared by Becky Jimenez NSF REU Civil Infrastructure Engineering, UH, Summer 2009 Background and Theory Site Details Bacteria Lab Processing Lab Processing Analysis Conclusion Total Coliform and E.coli
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Total Coliform and E.coli concentrations higher in
sediment than water
- Decreasing Sunlight Inactivation
- Protection from predation
- Protection from predation
- Increased nutrient availability
- Colonizable surface
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- E. COLI
Total Coliforms Fecal indicator bacteria
(FIB) (FIB)
- E. coli
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YSI READINGS UV READINGS
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Lab Lab Processing Processing
Matching samples according to time Dilutions (Different for Sediment) Adding reagent Shaking Placed into Quanti-trays and Sealed
24 Hour Incubation at 35 degrees
24 Hour Incubation at 35 degrees
Celsius
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WATER SAMPLES SEDIMENT SAMPLES
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Requirements:
- Geometric Mean < 126 MPN/dL
- Individual Samples cannot exceed 394 MPN/dL more than 25% of the time
2000 2500 3000
tion (MPN/dL)
Geometric Mean 500 1000 1500 2002 2003 2004 2005 2009
- E. coli Concentration
Years Geometric mean of E. coli concentrations for different years
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Cole Creek Samples Collected Samples > 394 MPN/__ % Exceeding Standard Geometric Mean Water (MPN/dL) 24 15 62.5 441 Sediment (MPN/kg) 24 24 100 110470
100 limit
- ver
samples
- f
number standard exceeding % × =
CONCENTRATION IN: TOTAL COLIFORM
- E. coli
SEDIMENT (MPN/kg) MINIMUM 238200 7100 MAXIMUM 24196000 1670000 WATER (MPN/dL) MINIMUM 19177 147 MAXIMUM 61410 2894
Maximum and minimum
- E. coli and total coliform
concentrations
100 evaluated samples total number standard exceeding % × =
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TOTAL COLIFORM CONCENTRATION IN WATER CHANGE WITH TIME FLUCTUATION OF TOTAL COLIFORM CONCENTRATION IN SEDIMENT
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VARIABILITY OF E. COLI CONCENTRATION IN WATER FLUCTUATION OF E. COLI CONCENTRATION IN SEDIMENT
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y = 1245.3e-2.38x R² = 0.666 200 400 600 800 6:00 AM 9:00 AM 12:00 PM 3:00 PM 6:00 PM
- E. coli concentration
(MPN/dL) Time Time and E. coli
- Expon. (Time and E. coli)
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Conclusions and Questions
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