Pla nt Re se a rc h Workshop Gre e nhouse Re se a rc h a nd L a - - PowerPoint PPT Presentation

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Sep 04, 2023 611 likes •957 views

Pla nt Re se a rc h Workshop Gre e nhouse Re se a rc h a nd L a bora tory Sa fe ty Offic e of Biologic al Safe ty Ca rrie Smith, Ph.D. Risk Ma na g e me nt Spe c ia list, Sr . Goals of Biosafe ty/ Biose c ur ity Pr ote c t pe r

SLIDE 1

Ca rrie Smith, Ph.D. Risk Ma na g e me nt Spe c ia list, Sr.

Pla nt Re se a rc h Workshop

Gre e nhouse Re se a rc h a nd L a bora tory Sa fe ty

Offic e of Biologic al Safe ty

SLIDE 2

Goals of Biosafe ty/ Biose c ur ity

 Pr

te c t pe r

sonne l

 Pre ve nt pe rso nne l fro m ha za rdo us ma te ria l

 Pr

te c t the e nvir
nme nt

 Co nta in re g ula te d ma te ria l inside the

g re e nho use

 Pr

te c t the inte gr

ity of the e xpe r ime nt

 Pre ve nt unwa nte d pe sts a nd c o nta mina nts fro m

e nte ring the g re e nho use

 Co nta in e xpe rime nta l ma te ria l to pre ve nt c ro ss

c o nta mina tio n o f e xpe rime nts

SLIDE 3

Re g ula te d Ma te ria ls

T r ansge nic or ganisms (GMOs)

 Pla nts  Mic ro b e s

 F

ung i

 Ba c te ria  Viruse s

 Anima ls

 Arthro po ds  Ne ma to de s

SLIDE 4

Re g ula te d Ma te ria ls

Othe r Mate r ials

 No n-Wisc o nsin

iso la te s o f pa tho g e ns

 E

xo tic spe c ie s

 No xio us we e ds  Huma n pa tho g e ns

a nd o ppo rtunistic pa tho g e ns

SLIDE 5

Risk Asse ssme nt

 Orig in  Sta b ility in e nviro nme nt o utside o f g re e nho use  T

ype a nd se ve rity o f dise a se (pa tho g e ns)

 T

ra nsmissib ility

 Ca pa b ility to c o ntro l if a re le a se o c c urs  Me a ns fo r c o nta inme nt

 Physic a l c o nta inme nt  Bio lo g ic a l c o nta inme nt  Pro c e dure s a nd pra c tic e s

SLIDE 6

Ove rsig ht

 Re g ula tory Ag e nc ie s  Inte rna l Ove rsig ht

 I

nstitutio na l Bio sa fe ty Co mmitte e (I BC)

 Offic e o f Bio lo g ic a l

Sa fe ty (OBS)

 Inte rna l Polic ie s

 UW I

nstitutio na l Bio sa fe ty Co mmitte e Ha ndb o o k

 Gre e nho use fa c ility

po lic ie s

SLIDE 7

Re g ula to ry a nd Guida nc e Do c ume nts

 NI

H Guide line s

 BMBL  Gre e nho use

c o nta inme nt g uide

 Arthro po d

c o nta inme nt g uide

SLIDE 8

T he Impor tanc e of Biosafe ty/ Biose c ur ity

 3 Go a ls o f Bio sa fe ty/ Bio se c urity

 Pro te c t the e nviro nme nt  Pro te c t pe rso nne l  Pro te c t inte g rity o f the re se a rc h

 No nc o mplia nc e c o nse q ue nc e s

 Suspe nsio n o r te rmina tio n o f re se a rc h a c tivitie s a nd

funding

 Re se a rc he r in vio la tio n  Othe r re se a rc he rs a t UW

 F

ine s (e .g . up to $250,000 pe r vio la tio n fo r no t a dhe ring to pe rmit re q uire me nts)

 Unive rsity  Re se a rc he r

 Da ma g e d pub lic pe rc e ptio n o f UW

SLIDE 9

Gre e nho use Bio sa fe ty/ Bio se c urity is a

Re se a rc h Pe rso nne l Gre e nho use Pe rso nne l I nstitutio na l Bio sa fe ty Co mmitte e Offic e o f Bio lo g ic a l Sa fe ty

SLIDE 10

What mate r ials ne e d to be c ontaine d?

I f the pla nt is tra nsg e nic ? I f the pla nt is ino c ula te d with a mic ro b e ?

SLIDE 11

How c an r e gulate d mate r ial ge t

ut of the gr

e e nhouse ?

Inse c ts Bir ds Stic k to c lothing Stic k to shoe s Down the dr ain Wind/ fans dispe r sal T hr

wn out be for

e inac tivation Contaminate d E quipme nt

SLIDE 12

Containme nt Str ate gie s

Physic a l Co nta inme nt Bio lo g ic a l Co nta inme nt Pra c tic e s a nd Pro c e dure s

SLIDE 13

Physic al Containme nt

Co nta inme nt c ha mb e r F a c ility struc ture

SLIDE 14

Physic a l Co nta inme nt

Wa te r c a pture de vic e Ve stib ule

SLIDE 15

Physic a l Co nta inme nt

Pla nt tra ys I nse c t do rm

SLIDE 16

Physic a l Co nta inme nt

Ba g re pro duc tive struc ture s Dra in so c k

SLIDE 17

Physic a l Co nta inme nt

Stic ky ma t Do o r swe e p

SLIDE 18

Biologic al Containme nt

 Stra te g ie s to ke e p tra nsg e ne s o ut o f

po lle n/ se e d

 Use ste rile line s  Use Vira l I

nduc e d Ge ne Sile nc ing (VI GS) to intro duc e tra nsg e ne s

 I

nse rt tra nsg e ne s into the c hlo ro pla st

Ste rile ma le F e rtile ma le

SLIDE 19

Biologic al Containme nt

 Muta nt mic ro b e s

 Virus la c king g e ne (s) re q uire d

fo r tra nsmissio n via inse c t

 Mic ro b e s with re duc e d ho st

ra ng e

 Mic ro b e s tha t c a nno t pro duc e

surviva l struc ture s

SLIDE 20

Biologic al Containme nt

 Gro w pla nts in a lo c a tio n

whe re if the re wa s a re le a se the pla nts wo uld no t survive

 Gro w pla nts during a

se a so n in whic h if the re wa s a re le a se the pla nts wo uld no t survive

 Gro w pla nts during a

time whe n ne a rb y se xua lly c o mpa tib le pla nts a re no t re c e ptive

SLIDE 21

SLIDE 22

SLIDE 23

SLIDE 24

Ho w a re the se pla nts diffe re nt?

WT T ra nsg e nic I no c ula te d with a virus

SLIDE 25

Communic ation and Signage

 T

ra nsg e nic vs. no ntra nsg e nic

 I

no c ula te d vs. unino c ula te d (inc lude da te )

 Pro je c t spe c ific re q uire me nts  Sig na g e o n g re e nho use b a y do o r  L

a b e ls o n pla nts/ tra ys o f pla nts

 Co nta c t info rma tio n

SLIDE 26

Ke e ping T hings T idy and Cle an

SLIDE 27

Contain Mate r ial Dur ing T r anspor t

SLIDE 28

Disposal/ Disinfe c tion Pr

c e dur

e s

T ra nsg e nic ma te ria l must b e “re nde re d b io lo g ic a lly ina c tive b e fo re dispo sa l” Pla nt ma te ria l, so il, po ts, a nd g ro wth a re a

SLIDE 29

E me r ge nc ie s/ Ac c ide nts

 Re le a se o f tra nsg e nic ma te ria l o utside o f

fa c ility

 Spills o utside o f c o nta inme nt  E

xpo sure to b io ha za rdo us ma te ria l

 F

irst a id a nd me dic a l a tte ntio n ta ke s prio rity

ve r re po rting

 F

irst re po rt is in a dditio n to the E mplo ye e ’ s Wo rk I njury a nd I llne ss Re po rt

SLIDE 30

Summar y

 3 Go a ls o f Bio sa fe ty/ Bio se c urity

 Pro te c t pe rso nne l  Pro te c t the e nviro nme nt  Pro te c t the inte g rity o f the

e xpe rime nt

 Co nta inme nt Stra te g ie s

 Physic a l Co nta inme nt  Bio lo g ic a l Co nta inme nt  Pra c tic e s a nd Pro c e dure s

SLIDE 31

T hings to Re me mbe r

 K

no w the b io lo g y o f the o rg a nisms yo u a re wo rking with

 Be a wa re o f the c o nditio ns se t fo rth b y

re g ula to ry a g e nc ie s a nd the I nstitutio na l Bio sa fe ty Co mmitte e fo r yo ur pa rtic ula r pro je c t

 Utilize g re e nho use pe rso nne l a nd the o ffic e o f

b io lo g ic a l sa fe ty, we a re he re to he lp!

SLIDE 32

Contac t Info

Ca rrie Smith, Ph.D. Risk Ma na g e me nt Spe c ia list, Sr. Offic e o f Bio lo g ic a l Sa fe ty E nviro nme nt, He a lth & Sa fe ty 890-4951 c smith@ fpm.wisc .e du www.e hs.wisc .e du