Optogenetic Stimulation and Humidity Logging for Sleep Research - - PowerPoint PPT Presentation

Optogenetic Stimulation and Humidity Logging for Sleep Research

Devin Cornell, Kyle Gassel, Catherine Kinchen Advisors: Dr. Mihail Cutitaru, Dr. Matthew Thimgan

Presentation Outline

• Customer and Project Motivation
• Humidity Logging Project
• Light Stimulation Project
• Live Demo
• Q & A

Customer

• Dr. Matthew Thimgan

○ Sleep research ■ Fly food supply affected by low humidity ■ Current light flashing setup is inaccurate and uneven

Motivation

• Optogenetics is used to study

the genetic nature of sleep

• Light stimulation induces

sleep in flies

• Determine if lifespan is

increased by more sleep

• The research requires a

controlled environment for growing Drosophila flies

Value Proposition

• Light Stimulation Project

○ Solution that can be used for future optogenetics research ○ Solution could be shared with other researchers in the field

• Humidity Project

○ Will give customer confidence that incubation is ideal for fly development ○ Humidity information could be shared with other researchers to provide value to the field

Overview - Humidity Logging

• Measuring humidity inside

fly incubation test tubes to help ensure their survival

Hardware Setup

• Arduino Uno
• DHT22 Humidity Sensors using

custom digital protocol

• Sunfounder SD card reader
• DS3231 RTC
• Custom pull-up board

Physical Design

• Sensors housed in 3D printed

environment

• Inserted directly into fly

incubation tubes

• Removable cap

Output Log

• Samples every 0.5

second

• 6 humidity and

temperature sensors

• Stored in text file, opens

properly formatted in Excel

• Outputs over serial for

debugging

Overview - Light Stimulation

• Flashing LED’s at flies

and monitoring their sleep patterns

• Trikinetics- popular
• ptogenetics

technology

Hardware Setup

• 8 very bright LEDs
• Flashing at ~10Hz
• NMOS switch controlled

by npn on output of RasPi

• Potentiometer to adjust

brightness

• Spacing for heat

dissipation

• Array draws ~17W

Software Architecture

• Raspberry Pi with web server

controls light flashing

• Existing computer controls

RPi via web interface

• External hardware needed to

control LED power level

Live Demo

Q&A

Concordiam Design Group

References

[1] Inagaki HK, Jung Y, Hoopfer ED, et al. Optogenetic control of Drosophila using a red-shifted channelrhodopsin reveals experience-dependent influences on courtship. Nat Methods. 2014;11(3):325-32. [2]http://ar.neuro.fchampalimaud.org/