Optogenetic Stimulation and Humidity Logging for Sleep Research - PowerPoint PPT Presentation

Optogenetic Stimulation and Humidity Logging for Sleep Research Devin Cornell, Kyle Gassel, Catherine Kinchen Advisors: Dr. Mihail Cutitaru, Dr. Matthew Thimgan

Presentation Outline ● Customer and Project Motivation ● Humidity Logging Project ● Light Stimulation Project ● Live Demo ● Q & A

Customer ● Dr. Matthew Thimgan ○ Sleep research ■ Fly food supply affected by low humidity ■ Current light flashing setup is inaccurate and uneven

Motivation ● Optogenetics is used to study the genetic nature of sleep ● Light stimulation induces sleep in flies ● Determine if lifespan is increased by more sleep ● The research requires a controlled environment for growing Drosophila flies

Value Proposition Light Stimulation Project ● Solution that can be used for future optogenetics research ○ Solution could be shared with other researchers in the field ○ Humidity Project ● Will give customer confidence that incubation is ideal for fly ○ development Humidity information could be shared with other researchers to ○ provide value to the field

Overview - Humidity Logging Measuring humidity inside ● fly incubation test tubes to help ensure their survival

Hardware Setup Arduino Uno ● DHT22 Humidity Sensors using ● custom digital protocol Sunfounder SD card reader ● DS3231 RTC ● Custom pull-up board ●

Physical Design Sensors housed in 3D printed ● environment Inserted directly into fly ● incubation tubes Removable cap ●

Output Log Samples every 0.5 ● second 6 humidity and ● temperature sensors Stored in text file, opens ● properly formatted in Excel Outputs over serial for ● debugging

Overview - Light Stimulation ● Flashing LED’s at flies and monitoring their sleep patterns ● Trikinetics- popular optogenetics technology

Hardware Setup 8 very bright LEDs ● Flashing at ~10Hz ● NMOS switch controlled ● by npn on output of RasPi Potentiometer to adjust ● brightness Spacing for heat ● dissipation Array draws ~17W ●

Software Architecture Raspberry Pi with web server ● controls light flashing Existing computer controls ● RPi via web interface External hardware needed to ● control LED power level

Live Demo

Q&A Concordiam Design Group

References [1] Inagaki HK, Jung Y, Hoopfer ED, et al. Optogenetic control of Drosophila using a red-shifted channelrhodopsin reveals experience-dependent influences on courtship. Nat Methods. 2014;11(3):325-32. [2]http://ar.neuro.fchampalimaud.org/