Optimization of Vesicle Optimization of Vesicle Adsorption to Form Lipid Adsorption to Form Lipid Bilayers Bilayers Ricardo Garcia, Allan Hancock College Ricardo Garcia, Allan Hancock College Mechanical Engineering Mechanical Engineering Faculty Advisor: Jacob Israelachvili Faculty Advisor: Jacob Israelachvili Lab Mentor: Younjin Lab Mentor: Younjin Min Min Funded by Corning Inc. Funded by Corning Inc.
Lipid Bilayers Lipid Bilayers A lipid bilayer is the outer A lipid bilayer is the outer structure of a cell membrane or structure of a cell membrane or vesicle consisting of a double vesicle consisting of a double layer of phospholipids layer of phospholipids molecules molecules Hydrophilic polar head Hydrophilic polar head Hydrophobic non- -polar tail polar tail Hydrophobic non http://www.uni-leipzig.de/~sfb294/Symposium2002/Vestext.htm Adsorption Adsorption http://homepage.mac.com/huntington.c/Images/lipidbilayer.gif http://img.sparknotes.com/figures/A/a981208a1abd542364d5a13c08702881/phospholipid.gif
Why are Lipid Bilayers so Important? Why are Lipid Bilayers so Important? Critical components in biology Critical components in biology � Outer structure of a cell Outer structure of a cell � � Main composition of a cell membrane Main composition of a cell membrane � � Protection Protection � � Endocytosis Endocytosis � Models for fundamental biological membranes Models for fundamental biological membranes Application in biosensors, semiconductors, Application in biosensors, semiconductors, protein mobilization protein mobilization
Goals Goals Help redesign and test the streaming potential Help redesign and test the streaming potential apparatus apparatus Experimentally determine the factors that Experimentally determine the factors that optimize the adsorption of vesicles onto a glass optimize the adsorption of vesicles onto a glass surface surface � Concentration of Lipid Concentration of Lipid � � Concentration of Aqueous Solution Concentration of Aqueous Solution � Understand the interactions between the Understand the interactions between the vesicles and surface as well as between the vesicles and surface as well as between the vesicles themselves vesicles themselves
Streaming Potential Streaming Potential This device is used to indicate when the glass substrate This device is used to indicate when the glass substrate is completely adsorbed with lipid bilayers bilayers. . is completely adsorbed with lipid Used as a tool to view adsorption of vesicles. Used as a tool to view adsorption of vesicles.
CAD Renderings CAD Renderings
How It Works How It Works ∆ V Flow _ Cl Flow Electric Field K + Conduction Current Flow Accumulation Streaming Current Silica Substrate
100 mM KCl with Old Teflon Piece 0.30 0.25 Streaming potential (mV) εζ E 0.20 = η P k 0.15 Experimental Theoretical 0.10 0.05 0.00 0 500 1000 1500 2000 2500 3000 Pressure (Pa) Streaming potentials increased as applied pressures increased and salt concentrations decreased Pressure increased as flow rate increases and channel size decreases
100 mM KCl with New Teflon Piece 0.25 0.20 Streaming potential (mV) 0.15 0.10 Experimental Theoretical 0.05 0.00 0 2000 4000 6000 8000 10000 12000 Pressure (Pa)
What Remains to be Done What Remains to be Done Complete my goals: Complete my goals: � Test under different concentration Test under different concentration � � Introduce vesicles Introduce vesicles � � Experimentally determine the factors that optimize the Experimentally determine the factors that optimize the � adsorption of vesicles onto a glass surface adsorption of vesicles onto a glass surface � Understand the interactions between the vesicles and Understand the interactions between the vesicles and � surface as well as between the vesicles themselves surface as well as between the vesicles themselves
Acknowledgments Acknowledgments Jacob Irealachvilli Irealachvilli Jacob INSET INSET UCSB UCSB National Science Foundation (NSF) National Science Foundation (NSF) California NanoSystem NanoSystem Insitute Insitute (CNSI) (CNSI) California Corning Inc. Corning Inc. Special Thanks to: Special Thanks to: Samantha Freeman Samantha Freeman Dr. Nick Arnold Dr. Nick Arnold Noshir Pesika Pesika Noshir Ranulfo Morales Morales Ranulfo
Thank You, Thank You, Questions? Questions?
Three Dimensional Bottom View Three Dimensional Bottom View
Three Dimensional View of Top Three Dimensional View of Top
Skeletal Side View Skeletal Side View
Skeletal View Skeletal View
Endocytosis Endocytosis Is the cellular Is the cellular procsess of engulfing procsess of engulfing solid or liquid particles solid or liquid particles by the cell membrane by the cell membrane to form an internal to form an internal “food vesicle food vesicle” ” “ http://www.mansfield.ohio-state.edu/~sabedon/039phago.gif
How it Works How it Works ∆ V + - Cl - Flow Electric Field Conducting Current Streaming Current Accumulation K + Charged Layer
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