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Angelina Chalima, PhD Student, National T echnical University of Athens
Optimization of production of DHA from Crypthecodinium cohnii - - PowerPoint PPT Presentation
Optimization of production of DHA from Crypthecodinium cohnii - - PowerPoint PPT Presentation
Optimization of production of DHA from Crypthecodinium cohnii utilizing a dark fermentation effmuent Angelina Chalima, PhD Student, National T echnical University of Athens The accumulation of waste 19% Bioconversion of biowaste to high
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Bioconversion of biowaste to high added-value products (carotenoids, omega-
3, biopolymers…)
Horizon 2020
Volatile Fatty Acids Platform from Biowaste by a dark fermentation process
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Dark fermentation
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Horizon 2020
Volatile Fatty Acids Platform from Biowaste by a dark fermentation process
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Crypthecodinium cohnii
- Marine dinofmagellate
- High lipid content (<70%)
- High percentage of DHA
(docosahexaenoic acid)
- Able to grow on glucose,
acetate, ethanol Used commercially for the production of DHA by Martek Bioscience
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So what did you actually do? We examined
- C. cohnii
growth on the main VFA
- f a DF
effmuent And then?
We developed a fermentation protocol in an actual DF effmuent
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Batch cultures of the strain
Acetic acid Propionic acid Butyric acid
Initial concentrations: 5- 50 g/L
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Low biomass production/ Specifjc tolerance to each VFA/ pH rise
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Fed-batch pH-auxostats
- 15 g/L sodium
acetate
- 7.5 g/L yeast
extract
- 25 g/L sea salts
Acetic acid (33% v/v) Propionic acid (25% v/v) Butyric acid (25% v/v) DF ultrafjltrated effmuent Synthetic medium
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- A. Chalima, et al. Integration of a dark fermentation
effmuent in a microalgal-based biorefjnery Applied Energy 241 (2019) 130-138
12 days 48 h
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Optimization
Culture conditions
Feed composition
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Optimization of lipid production (1. culture conditions)
- Difgerent T
20-30oC
- Difgerent nitrogen sources
Ammonium- Urea- Yeast extract- Nitrate
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Optimization of lipid production (1. culture conditions)
- Difgerent C/N ratio
10-140
- Chemical modulators
Ethanolamine Salicylic acid
C/N=50 DHA=36 %
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Optimization of lipid production (2. Feed)
- Higher acid concentration
DF effmuent Total VFA (g/L) #1 13.8 #2 24.6
48 h 60 h
28%
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Conclusions
- Acetate, butyrate, propionate
- DF effmuent
(utilization of total organic content) Higher VFA concentration is favorable
- Ammonium
- 27 oC
- C/N= 110
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Gazing into the future…
- Use of DF effmuent as an initial medium for the
bioreactors more effjcient utilization (in progress…)
- Examination of utilization of lactic acid
- Application of a two-stage fermentation protocol
“The future starts
today, not tomorrow…”
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Thank you very much!!
IndBioCat Group
Evangelos T
- pakas
Maria Dimarogona Anthi karnaouri Natasa Zerva Alicia Paz Stratos Nikolaivits Georgia Asimakopoulou Grigoris Dedes Christina Pentari
Volatile W.P 6 group
Magdalini Krokida Thomas Dietrich