Mo le c ula r a na lysis o f phlo e m tissue a nd its inte ra c tio - - PowerPoint PPT Presentation

Ag re e nSkills Annua l Me e ting – T

• ulo use – Oc to b e r 27-31, 2014

Mo le c ula r a na lysis o f phlo e m tissue a nd its inte ra c tio n with phyto pla sma s

F e de ric a De Ma rc o

INRA, Institut Je a n-Pie rre Bo urg in, UMR 1318, E RL CNRS 3559, Sa c la y Pla nt Sc ie nc e s, RD10, F

• 78026 Ve rsa ille s, F

ra nc e

Phlo e m, Allo c a tio n, T ra nspo rt a nd Sig na lling T e a m, Dr. Sylvie Dina nt

Phyto pla sma - phlo e m tissue inte ra c tio ns

From Sugio et al., 2011

PHLOEM

CO2 O2

Phloem sap Xylem sap

Photosynthesis

Source Sink Sink

PHYTOPLASMAS

0.7 µm

SIEVE ELEMENT – Conducting cell

PLANT DISEASE

A, B: healthy C, D: infected tomato. Pracros et al., 2006 Infected grapevine. Hren et al., 2009

Pro je c t o utline

Solanum lycopersicum ‘Ca. Phytoplasma solani’

OBJECTIVE: Decipher the trophic relationships between phytoplasmas and plant host

What are the plant genes modulated by the infection? What are the signalling pathways triggered by the infection? What nutrients are required for the infection?

SOURCE LEAF

Peptides Organic acids Effectors Effectors

PHYTOPLASMA

v n

COMPANION CELL SIEVE ELEMENT

Amino Acids Sugars Sugars 0.7 µm

Pro je c t o utline

Understanding of pathogenic mechanisms of ‘Ca. Phytoplasma solani’ and of tomato response to the infection Insights on tomato phloem physiology, carbohydrate transport and allocation

Design new strategies of resistance to phytoplasmas Identification of marker genes to select resistant cultivars Use of marker genes to detect the presence

• f phytoplasmas

POTENTIAL APPLICATIONS