SLIDE 1
LBE’s research activities
Christof Holliger
September 2018
ENAC – ISTE Laboratory for Environmental Biotechnology
LBEs research activities Christof Holliger September 2018 Two main - - PowerPoint PPT Presentation
LBEs research activities Christof Holliger September 2018 Two main - - PowerPoint PPT Presentation
ENAC ISTE Laboratory for Environmental Biotechnology LBEs research activities Christof Holliger September 2018 Two main research topics Aerobic granular sludge (AGS) for wastewater treatment Organohalide respiration of
SLIDE 2
SLIDE 3
Granules are compact biofilm structures
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after 1 min of settling
- more compact systems
- higher flexibility
- better settling characteristics
easier separation of sludge and water “Future of wastewater treatment”
Source: Water21, April 2013
- C, N and P removal within
- ne reactor
Advantages of AGS
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Process parameters Micro- environmental conditions C-P-N nutrient removal from wastewater granule bacterial community Multivariate statistical methods Multilevel correlations
Nitrifiers Denitrifiers Polyphosphate- accumulating
- rganisms (PAO)
Glycogen-accumulating
- rganisms (GAO)
Understanding aerobic granular sludge systems at the community level
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(enhanced) natural attenuation of chloroethenes contamination
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The anaerobic respiration hypothesis
0,5 (volts)
- 0,5
Reduction
CT to CF O2 to H2O PCE to TCE NO3 to NO2 DCB to MCB DCE to VC SO4 to H2S CO2 to CH4 Ferredoxin red. to ox NADH to NAD
Oxidation
Free energy (kJ/mol of electrons transferred
+50
- 50
0,5 (volts)
- 0,5
Reduction
CT to CF O2 to H2O PCE to TCE NO3 to NO2 DCB to MCB DCE to VC SO4 to H2S CO2 to CH4 Ferredoxin red. to ox NADH to NAD
Oxidation
Free energy (kJ/mol of electrons transferred
+50
- 50
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Tentative model of the tetrachloroethene respiration chain of Dehalobacter restrictus
HynC2
Fe Fe cyt b
HynS2 HynL2
Ni Fe Fe-S Fe-S Fe-S
PceA
Co Fe-S Fe-S
PceB « PceC »
Fe FAD
H2 2H+ ½PCE ½DCE + Cl- 2H+ 2H+ H+ 2e-
MKH2 MK
2e-
? ? H+/e- = 1.5
(measured for Dehalobacter: 1.25±0.2) HynC2
Fe Fe cyt b
HynS2 HynL2
Ni Fe Fe-S Fe-S Fe-S
PceA
Co Fe-S Fe-S
PceB « PceC »
Fe FAD
2H + ½PCE ½DCE + Cl- 2H+ 2H+ H+ 2e-
MKH2 MK
2e-
? ?
H+/e- = 1.5
(measuredfor Dehalobacter
: 1.25±0.2)
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