JOINT DEPFET Meeting Goettingen 24 ‐ 26 September, 2012 KEK ‐ Photon Factory Test Beams h High Energy Accelerator Research Organization Institute of Materials Structure Science Soichi Wakatsuki, Naohiro Matsugaki, Nobutaka Shimizu Institute of Particle and Nuclear Science Yutaka Ushiroda, Shuji Tanaka, Takeo Higuchi, Toru Tsuboyama, Hideki Miyake Max Planck Institute for Physics, Munich Hans-Günther Moser Christian Kiesling Jelena Ninkovic Hans-Günther Moser, Christian Kiesling, Jelena Ninkovic, Christian Koffmane, Felix Müller, Martin Ritter
Outline • Objective • March 2012 Experiments: short report March 2012 Experiments: short report • Next KEK ‐ PF test on November 16 ‐ 18, 2012 – Detector rotation stage Detector rotation stage – Handshaking for data collection – Image reconstruction program I t ti • To do list – Hideki Miyake will visit MPI München in Oct to learn the system – Completion of detector rotation stage with C l ti f d t t t ti t ith handshaking – Preparation of appropriate protein crystals Preparation of appropriate protein crystals • Summary
① Prep for developing the DEPFET detector Preliminary experiments to characterize DEPFET sensors for structural biology applications ① － Ⅰ High spatial resolution images of diffraction patterns Check of spatial resolution and peak shape with small beam matched for small xtals Small DEPFET sensor ① ① － Ⅱ Fast readout data acquisition for solution Ⅱ Fast readout data acquisition for solution 6.4mm × 0.8mm, 25 μ m square : 6 4mm × 0 8mm 25 μ m square : scatterin gexperiments 256 × 64 pixels Protein folding and photo excitation dyanmics followed by DEPFET time ‐ resolved SAXS with 20 sec time resolution time resolved SAXS with 20 sec time resolution X ‐ ray Design optimization of for large ‐ are DEPFET detector Design optimization of for large are DEPFET detector system Rotation • Spatial resolution, sensitivity, non ‐ uniformity, table dynamic range etc dynamic range etc. • Pseudo large solid angle data Comparison with commercially available detectors collection using a rotation stage
② Development of large area DEPFET detector ②－ Ⅰ Ultrafast readout system ② Ⅰ Ultrafast readout system Crystallo ‐ graphic Crystallo graphic On ‐ the ‐ fly integration of max analysis 8 bit/pixel 50,000 images (24 bit/pixel) Integration g Software for mode noise Large area censor reduction 1536 × 256 pixels 1536 × 256 pixels Max 1 Gbytes/sec Fast noise ADC reduction Fiber optics p Protein dynamics Fast continuous … … mode mode ②－ Ⅱ 8M pixel DEPFET detector based structural/dynamics analysis system 8M pixels with 20 X 線 DEPFET Xtal sensors Data acquisition and analysis
③ Applications to challenging targets ③－ Ⅱ Solution studies of domain association- Ⅱ Solution studies of domain association ③－ Ⅰ Structural analysis and ③ Ⅰ Structural analysis and ③ dissociation dynamics and kinetics of dynamics of membrane protein signalosomes complexes & large complexes Structural changes of g Structure dynamics of complexes involved in Structure dynamics of complexes involved in NEMO in complex with photo synthesis and respiratory chain ： photo linear ubiquitin chains: excitation dynamics NF ‐ ｋ B signal Photo excitation tranduction pathway p y involved in inflammation, Rahighi et al. Cell , 2009 apoptosis, and cancer Liq jet rapid IKK ・ IKK ・ mixing NEMO complex Linearly ubiquitylated protein substrate … … 20 20 μ sec time ti h resolution dynamics Ring solution scattering experiments
March 2012 Experiment: short March 2012 Experiment: short report report
h
Beam test on 16 ‐ 18 November, 2012 Objective: To measure diffracted beams from a protein crystal by a DEPFET sensor which is moved around to cover large solid angle. The collected sensor which is moved around to cover large solid angle. The collected images are merged to a single big image in the end. Protein Measurement sequence: crystal 1 1. X ‐ ray shutter is opened X ray shutter is opened 2. Protein crystal is exposed X ‐ ray (by typically 1 sec) and small portion of the ll ti f th diffracted beam is X ‐ ray detected and recorded by shutter DEPFET 3. X ‐ ray shutter is closed Rotation 4. DEPFET sensor is moved stage stage to the next position 5. Repeat from the step 1
Proposed detector handshaking and stage control time Step 1: asking DEPFET to start data acquisition by TTL Master control TCP/IP Shutter shutter DEPFET signal just after the shutter software (with controller server is opened. Exposure time is p p socket interface) socket interface) set slightly longer than the Shutter OPEN data acquisition time TTL (signal for data acquisition start) DAQ start Step 2: DEPFET stops data acquisition with a fixed number of frames and b f f d DAQ stop then the shutter is closed Shutter CLOSE Master control TCP/IP Step 3: moving the DEPFET Motion Stage software (with sensor to the next position controller server socket interface) and go to step 1
Concatenation of scanned images • DEPFET sensor will be translated and rotated to mimic a ll b l d d d cylindrical detector • Diffraction images will be projected to a plane and merged to • Diffraction images will be projected to a plane, and merged to form a planar data • Parameters: • Parameters: – Radius :100 mm – No. of scans: 40 = 4(sagital) by 10(tangential) No of scans: 40 = 4(sagital) by 10(tangential) – L1 sensor type is used 768*250pxl (44 8x12 5mm) *40 (images) = ~7 68M pxl 768 250pxl (44.8x12.5mm) 40 (images) = 7.68M pxl 13
How to project DEPFET pixels? p j p • Various types of pixels (in size, aspect ratio) can exist in the detector – Distribute intensities to the pixels in the projected image according to the ratio of related areas – The shape on the projected pixels is not taken in account Pixels in the projected image DEPFET pixels 14
Concatenation test (1) • A sample image is translated in an appropriate step and concatenated just for test (Note overlapped areas should have no ‘gap’ in the real experiment) 15
Concatenation test (2) • An image obtained in the last beam test is used for this concatenated test sample image p g 16
Composite images • The pixel size of the projected image will be the same as that of Th i l i f th j t d i ill b th th t f smaller DEPFET pixels • In Y direction it will be stretched by 1 15 to reflect projection • In Y direction, it will be stretched by 1.15 to reflect projection • Image overlap will be dealt with later 6144x5750 3072x2875 Program coded in Python Program coded in Python Execution time: 4m26s (7m24s) on Xeon X5680 3.33GHz 17
To Do List for Nov 16 ‐ 18 KEK ‐ PF exp. • Hideki Miyake will visit MPI München in October to learn the system • Completion of detector rotation stage: mechanical drawings of the DEPFET system for mounting drawings of the DEPFET system for mounting • Data acquisition with handshaking between DEPFET and the X ‐ ray camera (crystal and detector rotation and the X ‐ ray camera (crystal and detector rotation stages) • Preparation of appropriate protein crystals P ti f i t t i t l • Prep for detailed analysis of the protein data – To make DEPFET library (MPI) available in the concatenating software – Data processing (indexing and integration of diffraction spots) 18
Summary • Preparation in progress for the November test beam at KEK Photon Factory: detector rotation beam at KEK Photon Factory: detector rotation stage, data acquisition, and data merging • We would appreciate it very much if a • We would appreciate it very much if a operating set of equipment at KEK (DEPFET matrix + PS + R/O) can be left at KEK, / ) b l f preferentially for a few months, for us to learn and continue the development for photon science science Thank you for your attention and y y support! 19
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