KEK Photon Factory Test Beams h High Energy Accelerator Research - - PowerPoint PPT Presentation

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JOINT DEPFET Meeting Goettingen 24‐26 September, 2012

KEK‐Photon Factory Test Beams

High Energy Accelerator Research Organization Institute of Materials Structure Science Soichi Wakatsuki, Naohiro Matsugaki, Nobutaka Shimizu Institute of Particle and Nuclear Science Yutaka Ushiroda, Shuji Tanaka, Takeo Higuchi, Toru Tsuboyama, Hideki Miyake Max Planck Institute for Physics, Munich Hans-Günther Moser Christian Kiesling Jelena Ninkovic Hans-Günther Moser, Christian Kiesling, Jelena Ninkovic, Christian Koffmane, Felix Müller, Martin Ritter

Outline

• Objective
• March 2012 Experiments: short report

March 2012 Experiments: short report

• Next KEK‐PF test on November 16‐18, 2012

Detector rotation stage – Detector rotation stage – Handshaking for data collection I t ti – Image reconstruction program

• To do list

– Hideki Miyake will visit MPI München in Oct to learn the system C l ti f d t t t ti t ith – Completion of detector rotation stage with handshaking Preparation of appropriate protein crystals – Preparation of appropriate protein crystals

• Summary

① Prep for developing the DEPFET detector

Preliminary experiments to characterize DEPFET sensors for structural biology applications

① － Ⅰ High spatial resolution images of diffraction patterns

Small DEPFET sensor 6 4mm×0 8mm 25μm square:

Check of spatial resolution and peak shape with small beam matched for small xtals

① －Ⅱ Fast readout data acquisition for solution

6.4mm×0.8mm, 25μm square: 256×64 pixels

① Ⅱ Fast readout data acquisition for solution scatterin gexperiments

Protein folding and photo excitation dyanmics followed by time resolved SAXS with 20 sec time resolution DEPFET

Design optimization of for large‐are DEPFET detector

time‐resolved SAXS with 20 sec time resolution X‐ray

Design optimization of for large are DEPFET detector system

• Spatial resolution, sensitivity, non‐uniformity,

dynamic range etc

Rotation table

dynamic range etc.

• Comparison with commercially available detectors

Pseudo large solid angle data collection using a rotation stage

② Development of large area DEPFET detector

Crystallo graphic ②－Ⅰ Ultrafast readout system On‐the‐fly integration of max 50,000 images (24 bit/pixel) 8 bit/pixel Crystallo‐graphic analysis

Integration

② Ⅰ Ultrafast readout system Large area censor 1536×256 pixels Software for noise reduction

g mode

1536×256 pixels Fiber optics Max 1 Gbytes/sec ADC Fast noise reduction p

… …

Protein dynamics

Fast continuous mode mode

②－Ⅱ 8M pixel DEPFET detector based structural/dynamics analysis system X線 8M pixels with 20 DEPFET Xtal sensors Data acquisition and analysis

③ Applications to challenging targets

③－Ⅱ Solution studies of domain association- ③－Ⅰ Structural analysis and ③ Ⅱ Solution studies of domain association dissociation dynamics and kinetics of signalosomes ③ Ⅰ Structural analysis and dynamics of membrane protein complexes & large complexes

Structural changes of Structure dynamics of complexes involved in g NEMO in complex with linear ubiquitin chains: NF‐ｋB signal tranduction pathway Structure dynamics of complexes involved in photo synthesis and respiratory chain： photo excitation dynamics

Photo excitation

p y involved in inflammation, apoptosis, and cancer

Rahighi et al. Cell, 2009

Liq jet rapid mixing IKK・IKK・ NEMO complex Linearly ubiquitylated protein substrate

20 ti

… …

20 μsec time resolution dynamics solution scattering experiments

Ring h

March 2012 Experiment: short March 2012 Experiment: short report report

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Beam test on 16‐18 November, 2012

Objective: To measure diffracted beams from a protein crystal by a DEPFET sensor which is moved around to cover large solid angle. The collected sensor which is moved around to cover large solid angle. The collected images are merged to a single big image in the end.

Protein crystal

Measurement sequence:

1 X ray shutter is opened

X‐ray

• 1. X‐ray shutter is opened
• 2. Protein crystal is exposed

(by typically 1 sec) and ll ti f th

X‐ray shutter

small portion of the diffracted beam is detected and recorded by

Rotation stage

DEPFET

• 3. X‐ray shutter is closed
• 4. DEPFET sensor is moved

stage

to the next position

• 5. Repeat from the step 1

Proposed detector handshaking and stage control

time

DEPFET Step 1: asking DEPFET to start data acquisition by TTL signal just after the shutter is opened. Exposure time is Master control software (with socket interface) Shutter controller shutter server

TCP/IP

p p set slightly longer than the data acquisition time socket interface)

TTL (signal for data acquisition start) DAQ start Shutter OPEN

Step 2: DEPFET stops data acquisition with a fixed b f f d number of frames and then the shutter is closed

Shutter CLOSE DAQ stop

Master control software (with socket interface) Motion controller Stage server Step 3: moving the DEPFET sensor to the next position and go to step 1

TCP/IP

ll b l d d d

Concatenation of scanned images

• DEPFET sensor will be translated and rotated to mimic a

cylindrical detector

• Diffraction images will be projected to a plane and merged to
• Diffraction images will be projected to a plane, and merged to

form a planar data

• Parameters:
• Parameters:

– Radius :100 mm No of scans: 40 = 4(sagital) by 10(tangential) – No. of scans: 40 = 4(sagital) by 10(tangential) – L1 sensor type is used

768*250pxl (44 8x12 5mm) *40 (images) = ~7 68M pxl 768 250pxl (44.8x12.5mm) 40 (images) = 7.68M pxl

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How to project DEPFET pixels?

• Various types of pixels (in size, aspect ratio) can exist in the detector

– Distribute intensities to the pixels in the projected image according to the

p j p

ratio of related areas – The shape on the projected pixels is not taken in account

Pixels in the projected image

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DEPFET pixels

Concatenation test (1)

• A sample image is translated in an appropriate step and

concatenated just for test (Note overlapped areas should have no ‘gap’ in the real experiment)

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Concatenation test (2)

• An image obtained in the last beam test is used for

this concatenated test sample image p g

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Composite images

Th i l i f th j t d i ill b th th t f

• The pixel size of the projected image will be the same as that of

smaller DEPFET pixels

• In Y direction it will be stretched by 1 15 to reflect projection
• In Y direction, it will be stretched by 1.15 to reflect projection
• Image overlap will be dealt with later

Program coded in Python 3072x2875 6144x5750

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Program coded in Python Execution time: 4m26s (7m24s) on Xeon X5680 3.33GHz

To Do List for Nov 16‐18 KEK‐PF exp.

• Hideki Miyake will visit MPI München in October to

learn the system

• Completion of detector rotation stage: mechanical

drawings of the DEPFET system for mounting drawings of the DEPFET system for mounting

• Data acquisition with handshaking between DEPFET

and the X‐ray camera (crystal and detector rotation and the X‐ray camera (crystal and detector rotation stages) P ti f i t t i t l

• Preparation of appropriate protein crystals
• Prep for detailed analysis of the protein data

– To make DEPFET library (MPI) available in the concatenating software – Data processing (indexing and integration of diffraction spots)

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Summary

• Preparation in progress for the November test

beam at KEK Photon Factory: detector rotation beam at KEK Photon Factory: detector rotation stage, data acquisition, and data merging

• We would appreciate it very much if a
• We would appreciate it very much if a
• perating set of equipment at KEK (DEPFET

/ ) b l f matrix + PS + R/O) can be left at KEK, preferentially for a few months, for us to learn and continue the development for photon science science

Thank you for your attention and

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y y support!