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Intracellular potentials Extracellular Intracellular Intracellular - PowerPoint PPT Presentation

Oct 04, 2022 •270 likes •534 views

Intracellular potentials Extracellular Intracellular Intracellular microelectrotrode Replacing air with KCl in electrodes Intracellular microelectrotrode 0.1 micron Electrode resistance (10 to 30 Mohms) RC response of

  1. Intracellular potentials �

  2. Extracellular � Intracellular �

  3. Intracellular microelectrotrode �

  4. Replacing air with KCl in electrodes �

  6. Intracellular microelectrotrode � 0.1 micron � Electrode resistance (10 to 30 Mohms) �

  7. RC response of electrode to current injection � Time constant? �

  8. Electrode capacitance �

  9. Intracellular � Extracellular � direct charge transfer � capacitative charge transfer � DC charge transfer on electrode wire � Conduction in saline solution � Ag + + Cl - � AgCl � Very low solubility � +e - -e - � Conduction in metal � � Ag �

  10. Current injection into cells �

  11. Resting Potential � O mV � What do these have in common? � � “rubbing salt in a wound” � � Lethal injection cocktail � � Dr. Kevorkian’s suicide machine � � Hyperkalemia � � Hypokalemia �

  12. Membrane ion distributions �

  13. Ionic basis of the resting potential � Unequal distribution of ion species across membrane �

  14. Donnan Equilibrium � Selective permeability of ion species through membrane � Loss of K??- Q = CV 10-5 M K for -58 mV �

  18. Squid giant axon- a model prep �

  20. 40,000 APs can fire! �

  22. At Rest: INa = IK? � � IK = gK (Vm-EK) INa = gNa (Vm-ENa) � gK (Vm-EK) = gNa (Vm-ENa) �

  23. Other contributions to RP? �

  25. Changes in “ RP” by changing ionic conductances �

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