INSTITUTE VPH dept. Laboratory for Food Microbiology Andrea - - PowerPoint PPT Presentation

CROATIAN VETERINARY INSTITUTE

CROATIAN VETERINARY INSTITUTE

VPH dept. Laboratory for Food Microbiology

Andrea Humski, Ph.D, DVM Marina Mikulić, DVM

CROATIAN VETERINARY INSTITUTE

about CVI... Established in 1933. Leading national diagnostic, research and scientific institution in the field of veterinary medicine

• Ministry of Agriculture, Fishery and Rural Development

(MPRRR)

• Ministry of Science, Education and Sports (MZOS)

CROATIAN VETERINARY INSTITUTE

AREAS OF OPERATION

• 1. DIAGNOSTIC AND ANALYTICAL

ACTIVITIES

Preparedness, field and laboratory diagnostics, profilaxis, surveillance and monitoring, data analysis, planing and proposal of preventive actions, reference and scientific advisory functions in control of:

• Infectious diseases
• Zoonoses
• Food and feed safety
• Veterinary drugs and residues

CROATIAN VETERINARY INSTITUTE

• 2. SCIENTIFIC AND RESEARCH ACTIVITIES

 Performed within the framework of The National scientific

programme

 Executed through the several scientific research projects

financially supported by Ministry of science, education and sport

CROATIAN VETERINARY INSTITUTE

CVI laboratory network

...employees total no. – 238

• PhD – 34
• Msc – 13
• Sci. advisor – 7
• Senior sci. associate - 10
• Sci. associate – 11

CROATIAN VETERINARY INSTITUTE

Vinkovci Križevci CP

CROATIAN VETERINARY INSTITUTE

CVI Zagreb

• I. VPH Dept.

I.1. Laboratory for food microbiology I.2. Laboratory for residue control I.3. Laboratory for feed microbiology I.4. Laboratory for analytical chemistry I.5. Laboratory for veterinary medicinal products

CROATIAN VETERINARY INSTITUTE

LABORATORY FOR FOOD MICROBIOLOGY, Zagreb

• microbiological control of food: official control (import/export),
• n request (domestic production/market)
• monitoring programmes

– microbiological contamination of meat in slaughterhouses – microbiology food safety of products- veterinary inspection on border posts

• research and scientific work

CROATIAN VETERINARY INSTITUTE

11 Enterobacteriaceae –Part 1.: MPN method ISO 21528-1:2004 12 Enterobacteriaceae – Part 2.: Colony count technique ISO 21528-2:2004 13 Staphylococcus aureus and other species – Part 1.: Technique using Baird-Parker medium HRN EN ISO 6888-1:2004 14 Sulfite-reducing bacteria HRN ISO 15213:2004 15 Clostridium perfringens - Colony count technique HRN EN ISO 7937: 2005 16 Campylobacter spp. – Part 1. Detection method ISO 10272-1:2006 17 Campylobacter spp. – Part 2. Enumeration method ISO 10272-2:2006 18 Campylobacter spp. TECRA VIA – Screening method

Microbiological parameter/method

1 Salmonella spp. HRN EN ISO 6579:2003 / cor 2004 2 Salmonella spp. TECRA VIA – Screening method AOAC Official Method 989.14;998.09 3 Listeria monocytogenes – Part 1.: Detection method HRN EN ISO 11290 - 1:1999 4 Listeria monocytogenes – Part 2.: Enumeration method HRN EN ISO 11290 - 2: 1999 5 Listeria spp. TECRA VIA – Screening method AOAC Official Method 995.22;2002.09 6 Enumeration of microorganisms - CCT at 30°C HRN ISO 4833:2003 7 Escherichia coli – Part 2.:Colony count technique at 44 °C HRN ISO 16649-2:2001 8 Escherichia coli – Part 3.: MPN method ISO/TS 16649-3:2005 9 Escherichia coli O157 HRN EN ISO 16654:2003 10

• E. coli O157. TECRA VIA – screening method

AOAC –RI, L.NO.001101

LABORATORY FOR FOOD MICROBIOLOGY ACCREDITATION CERTIFICATE

CROATIAN VETERINARY INSTITUTE

External Quality Assesment/Proficiency Testing

Health Protection Agency, London, UK (since 2005.)

Parameter

• No. of tested samples

Salmonella spp. 15 Listeria monocytogenes / E 8 Listeria monocytogenes / D 4

• E. coli, MPN

9

• E. coli / E

2

• E. coli O157

3 ACC 11 Campylobacter spp. 6

• Coag. + Staphylococcus / E

5

• Staph. aureus enterotoxin

2

• Cl. perfringens / E

3 Enterobacteriaceae / E 3

CROATIAN VETERINARY INSTITUTE

Present and future activities….

CROATIAN VETERINARY INSTITUTE

Zoonoses in humans 2005 - 2008

Disease 2005 Cases Deaths Rate per 100,000 population Rank Salmonellosis 5619 124.9804 1 Leptospirosis 126 2.8026 2 Q fever 40 0.8897 3 Toxoplasmosis 39 0.8675 4 Trichinellosis 27 0.6005 5 Disease 2006 Cases Deaths Rate per 100,000 population Rank Salmonellosis 4734 1 105.2958 1 Trichinellosis 45 1.0009 2 Leptospirosis 39 0.8675 3 Toxoplasmosis 31 0.6895 4 Q fever 28 0.6228 5 Disease 2007 Cases Deaths Rate per 100,000 population Rank Salmonellosis 3331 74.0897 1 Campylobacteriosis 141 3.1362 2 Leptospirosis 63 1 1.4013 3 Q fever 43 0.9564 4 Toxoplasmosis 31 0.6895 5 Disease 2008 Cases Deaths Rate per 100,000 population Rank Salmonellosis 3664 81.4964 1 Campylobacteriosis 1294 28.7818 2 Q fever 41 0.9119 3 Brucellosis 25 0.5561 4 Toxoplasmosis 24 0.5338 5

CROATIAN VETERINARY INSTITUTE

500 1000 1500 2000 2500 3000 3500 4000 4500 5000 5500 6000

2005 2006 2007 2008

Salmonellosis Campylobacteriosis

CROATIAN VETERINARY INSTITUTE

• 1. Monitoring programme - Ordinance on conduction of veterinary inspection

and control of animal products in traffic across the border of the Republic of Croatia (O.G. 132/08) / Ordinance on the document for registration of veterinary health inspection of shipments of animal origin entering the Republic of Croatia (O.G.129/06 and 75/07) Annex II

• 2. Baseline study acc. to Commission Decision 2007/516/EC: Campylobacter

and Salmonella prevalence in broiler flocks (carcasses + caecal content)

CROATIAN VETERINARY INSTITUTE

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