INSTITUTE OF PUBLIC HEALTH INSTITUTE OF PUBLIC HEALTH Institute for - - PowerPoint PPT Presentation

INSTITUTE OF PUBLIC HEALTH INSTITUTE OF PUBLIC HEALTH

Institute for Public Health of Montenegro – Centre For Disease Control and Prevention

Center for Medical Microbiology Center for Medical Microbiology

Department for Department for Virology and Serology Virology and Serology

Serodiagnostic of microorganisms

Department for Department for Molecular Diagnostics Molecular Diagnostics

Molecular diagnostic of most important medical microorganisms Preparing for cell culture and virus isolation most important medical pathogens

• Assoc. Prof.
• Assoc. Prof. Boban

Boban Mugo Mugoš ša, a, responsible responsible person person person person

• Assoc. Prof.
• Assoc. Prof. Zoran

Zoran Vratnica Vratnica, key researcher , key researcher

• Dr.
• Dr. Danijela

Danijela Vujo Vujoš ševi ević ć, researcher , researcher

• Dr.
• Dr. Sanja

Sanja Medenica Medenica, researcher , researcher

• Dr.
• Dr. Neboj

Nebojš ša a Sekuli Sekulić ć, researcher , researcher

WP1c Detection of pathogens in mosquitoes and humans

University of Montenegro Biotechnical faculty

VISITS OF IPH RESEARCHERS TO INSTITUTE "ISTITUTO SPERIMENTALE ZOOPROFILATTICO REGGIO EMILIA" (IZSLER), 14 JULY - 01 AUGUST 2014

WP1c - Realized activities

Preparation of mosquitoes pools for RNA extraction; Random primer Retro-Transcription reaction protocol assessment; Real Time PCR protocols and reactions set-up; Real Time PCR protocols and reactions set-up; Real Time PCR protocols and results interpretation; RNA extraction with semi-automatic RNA/DNA extractor.

RNA extraction

Manualy (TRIzol LS Reagent)

Semi-automatic extractor

Random primer Retro-Transcription reaction

Real Time PCR reactions set-up and results interpretation

Results of molecular analyses from Montenegrian mosquites

Pathogen IgM ELISA IgG ELISA Microscopy Real-Time PCR

WNV 48 tests (all negative) 48 tests (all negative) / / Borrelia burgdorferi 838 tests 840 tests / 76 tests Borrelia burgdorferi 838 tests (79 positive) 840 tests (59 positive) / 76 tests (all negative) Leishmania spp. / / 22 (all negative) / Leishmania donovani / 143 tests (10 positive) / / Plasmodium spp / / 14 (all negative) /

INSTITUTE OF PUBLIC HEALTH MONTENEGRO INSTITUTE OF PUBLIC HEALTH MONTENEGRO Centre for Disease Control and Prevention

Mandatory reporting diseases - electronic database 2014

Lyme boreliosis – six reports of disease Leishmaniasis – three reports of disease Infectio West Nile – no registered cases Malaria – no registered cases

Equipment for IPH

QuantStudio™ 7 Flex Real-Time PCR System, Applied Biosystems

ELISA Analyzer I-2P EUROIMMUN

THANK YOU!

Thank you!