in vivo Imaging Using Nanoparticles Keon Wook Kang, MD, PhD - PowerPoint PPT Presentation

in vivo Imaging Using Nanoparticles Keon Wook Kang, MD, PhD Department of Nuclear Medicine & Cancer Research Institute Seoul National University College of Medicine

Failure of Nanomedicine MAY 2016

Nanoparticle Imaging in Nuclear Medicine Bone marrow scan Liver scan Lymphoscintigraphy

Biodistribution of nanoparticles 30 nm silica nanoparticles in a mouse NIR Imaging Before injection 1 h after injection 3 h after injection PET/CT Just after injection 1hr after injection 2hr after injection 30 nm gold nanoparticles in a mouse

125 I-labeled cyclic RGD peptide- gold nanoparticle In vivo SPECT/CT imaging of in a U87MG tumor bearing mouse 125 I-cRGD-gold 125 I-gold Cold blocking test nanoparticles nanoparticles tumor tumor tumor Small 2011

FDA approved nano-drugs

99m Tc-albumin scan in human 5 min 10 min 15 min 30 min 60 min 2 h 4 h 24 h

99m Tc-albumin scan in mouse 0.5 h 4 h 13 h 24 h Capan1

Liver targeting: Exploiting the asialoglycoprotein receptor for drug delivery to hepatocellular carcinomas • The asialoglycoprotein receptor is expressed in ~ 80 % of hepatocellular carcinomas. • Felix Kratz in cooperation with Prof. Luigi Fiume and Dr. G. Di Stefano Fiume et al.: J. Hepatology 43, 645-652, 2005; Di Stefano et al., Europ.J. Pharm. Sciences 23, 393-397, 2004 ; Di Stefano et al., Liver Int. 24, 246-252, 2004; Di Stefano et al., Digestive and Liver Disease 35, 428-33, 2003 . O H Galactose HSA S N N N O OH 20 O O OH OH O OH O L-HSA-DOXO-EMCH O O NH 2 6 OH

64 Cu-lactoamine-albumin PET 64 Cu-HSA 64 Cu-L8-HSA 64 Cu-L56-HSA HT29 Hep3B HepG2 46 h after injection

Versatile and Finely Tuned Albumin Nanoplatform based on Click Chemistry

64 Cu labeled Albumin Platform PET imaging 0 h 2 h 4 h 24 h 48 h HSA Galactose-HSA Mannose-HSA

Hepatocyte vs Kupffer cell GSA - Cy3 MSA - FNR-648

SPARC in cell lines RT-PCR Glioma cells SPARC knock down cell western blot Glioma cells 43kDa SPARC Beta-actin

in vivo fluorescent imaging HSA-FNR648 injection via tail vein 0 h 4 h 8 h 24 h U87MG SPARC KD U87MG 6.0 x 5.9 mm 2 6.0 x 6.3 mm 2

ex vivo fluorescent imaging U87MG U87MG -SPARC KD *V: blood vessel Theranostics 2019

Precision nanomedicine using molecular imaging NH 2 Nano-carrier Genomics Targeting biomarker In vivo molecular imaging Precision theranostics

Nuclear Medicine, SNU Faculties: 22 MD: 12 / PhD: 10 (Physics, Chemistry, Molecular Biology) MD PhD GJ Cheon KW Kang WW Lee JS Lee BC Lee DS Lee SE Kim JM Jeong HW Youn JC Paeng YK Kim HY Lee SW Oh DW Hwang YH Kim HY Choi YS Lee MG Song Researchers: 42