fungal spore morphology
play

Fungal Spore Morphology Estelle Levetin, PhD Disclosure No - PowerPoint PPT Presentation

Session 1202: Basic Aeroallergen Course Fungal Spore Morphology Estelle Levetin, PhD Disclosure No conflicts to disclose Fungal Spore Characteristics Characteristics Spore size Spore shape Number of cells Attachment Scars


  1. Session 1202: Basic Aeroallergen Course Fungal Spore Morphology Estelle Levetin, PhD

  2. Disclosure • No conflicts to disclose

  3. Fungal Spore Characteristics

  4. Characteristics • Spore size • Spore shape • Number of cells • Attachment Scars • Wall characteristics • Spore color

  5. Spore Size, Shape, and Septation • SIZE : 2 m m to 100 mm • SHAPE : Globose, elliptical, fusiform, asymmetric, lemon-shaped, barrel-shaped, curved • SEPTATION : Non-septate (one cell), single septum, transverse septa, transverse and longitudinal septa, random septa, pseudoseptate

  6. Other Characteristics • ATTACHMENTS : Attachment scars, attachment pegs • APPENDAGES • WALL CHARACTERISTICS: Smooth, granular, reticulate, spines, warts, wall thickness • COLOR : Hyaline (colorless) to deeply pigmented

  7. Globose Barrel-shaped Non-septate Random septa Lemon-shaped Club-shaped Transverse septa Attachment scars Elliptical Curved Transverse and Ornaments: spines Longitudinal septa Cylindrical Pseudoseptate Appendages Asymmetric and germ pore

  8. Spore color

  9. Asexual Spores Also known as Deuteromycetes, Fungi Imperfecti (imperfect fungi), or Mitospores

  10. Asexual Spores • Typically the most abundant spores in the atmosphere • Conidia often formed on specialized hyphae called conidiophores • Asexual stage of ascomycetes • Look for attachment scars where the spores were attached to the conidiophore or to each other

  11. Cladosporium

  12. Cladosporium Note the septum

  13. Several species of Cladosporium are common in the atmosphere

  14. Note the prominent attachment scars on Cladosporium conidia

  15. Alternaria

  16. Alternaria

  17. Curvularia

  18. Nigrospora Curvularia Drechslera

  19. Drechslera-type spores • Several genera of fungi have similar cylindrical spores – Drechslera – Bipolaris – Exserohilum – Helminthosporium

  20. Drechslera- type spores

  21. Pithomyces

  22. Note the colorless attachment at the base of Pithomyces spores

  23. Pithomyces in culture

  24. Epicoccum

  25. Epicoccum spore cluster (sporodochium)

  26. Penicillium species • Produce distinctive conidiophores (spore bearing structures) • Spores are usually spherical to oval and form in chains

  27. Aspergillus species • Produce distinctive conidiophores (spore bearing structures) • Spores are usually spherical to oval and form in chains

  28. Penicillium-Aspergillus type spores

  29. Penicillium-Aspergillus type spores

  30. Aspergillus niger spores Aspergillus niger in culture

  31. Fusarium

  32. Botrytis

  33. Oidium

  34. Other Asexual Spores on the NAB Count sheet

  35. Cercospora

  36. Nigrospora Nigrospora Culture Air Sample

  37. Periconia

  38. Polythrincium Peronospora

  39. Stemphylium

  40. Torula

  41. Other Asexual Spores NOT on the NAB Count Sheet

  42. Spegazzinia

  43. Tetraploa

  44. Pestalotiopsis

  45. Stachybotrys

  46. Division Ascomycota

  47. Eight ascospores are produced in each ascus without any attachment scars

  48. Ascospores are sometimes found in groups of eight in air samples

  49. *Leptosphaeria ascospores

  50. *Chaetomium ascospores

  51. *Pleospora ascospores

  52. Diatrypella ascospores (*Family Diatrypaceae)

  53. Phaeosphaeria annulata Bright field Phase contrast

  54. Sporomiella ascospores

  55. Venturia ascospores

  56. Paraphaeosphaeria ascospore

  57. Leptosphaerulina ascospore

  58. Various ascospores

  59. Many ascospores on a rainy day

  60. Division Basidiomycota

  61. Attachment Basidiospore Basidium

  62. *Ganoderma basidiospores

  63. Other basidiospores that are easy to recognize * Coprinus-type *Agrocybe- type

  64. Coprinus and Ganoderma basidiospores

  65. Psathyrella velutina Psathyrella Russula Stropharia

  66. Boletus Thelephora Chlorophylum Galerina

  67. Lycoperdon Calvatia Pisolithus Scleroderma

  68. Mixed Basidiospores

  69. Rust spores: Puccinia Puccinia uredospores Puccinia teliospores

  70. Smut Spores

  71. Smut Spores

  72. Myxomycete (slime mold) spores

  73. Other slime mold spores

Recommend


More recommend