Flow Culture System Multi-Disciplinary Senior Design System Design - PowerPoint PPT Presentation

Flow Culture System Multi-Disciplinary Senior Design System Design Review October 2, 2014 Collin Burkhart Katelyn Busse Michelle Garofalo Robert Repetti Morgan Stoessel Sarah Tran

Agenda ● CRs and ERs ● System Architecture ● Functional ● Alternative Concepts ● Feasibility Analysis Decomposition ● Morphological Chart ● FMEA ● Benchmarking ● Risk Assessment ● Concept ● Project Plan ● Deliverables Generation/Pugh ● Questions Analysis ● Turning Point/Concept Selection

Essential Customer Requirements

Essential Engineering Requirements

Functional Decomposition

View Cells

Culture Cells

Control Environment

Morphological Chart-1

Morphological Chart-2

Morphological Chart-3

Benchmarking - Whole System

Benchmarking - Whole System

Benchmarking - Pumps

Benchmarking - Materials

Benchmarking Shear Measurement and Control Requirements Shear Range: 0-20 dynes/cm 2 = 0-2.9X10 -4 psi Flow Range: 0-5.34 mL/second System Temperature: 25-37 ℃ Autoclave Temperature: Max 121 ℃ Strain Gauge Load Transducer Conversion Table Can it handle autoclave No Yes N/A and system temperatures? Can it measure a low force No (0.2 psi) No (0.1 psi) Yes (Flow Sensor) value? Can a display be made? Yes Yes Yes Is it cost feasible? <500 Yes ($160) No ($500) Yes (Free)

Concept Generation-Pugh Analysis

Turning Point

Concept Selection

System Incubator Water CO 2 Heat Architecture Pan Cells Filter Microscopes Cell Chamber Alternate Reservoir Media Media Reservoir Pump Architecture Color Electricity Structure Energy Media Color Flow Information Rate Materials

Alternative Cell Viewing Concepts Keep Attached for Viewing Detach from System for ● Have to move Viewing ● Increase risk for microscope next to contamination incubator ● Close off chamber ● Extra length of hose to ○ clamps on tubing (2 take out of incubator on each side) and set on microscope ○ Valves ● Carry total system on a ○ Caps (like flasks tray currently use)

Alternative Subsystem Concepts Media Circulation System

Feasibility Analysis-Pumps Syringe Pump Peristaltic Pump

Feasibility Analysis-Shear Stress/Flow Rates ● Values used for calculation o μ=0.78x10^ -3 Ns/m^2 o b=0.06m o a=0.0125m o T=2 N/m^2 ● Flow Rate T=shear stress μ=viscosity of the liquid o .016 L/s Q=flow rate b=channel width ● Dependent on Chamber Size a=½ channel height 1. Kojima, Naoya, Mitsuru Shiota, Yoshito Sadahira, Kazuko Handa, and Sen-itiroh Hakomori. "Cell Adhesion in a Dynamic Flow System Compared to Static System." The Journal of Biological Chemistry 267.24 (1992): 17264-7270. Web. 1 Oct. 2014. 2. Wang, Chong, Hao Lu, and Martin Alexander Schwartz. "A Novel in Vitro Flow System for Changing Flow Direction on Endothelial Cells." Journal of Biomechanics 45.7 (2012): 1212-218. ELSEVIER . Web. 1 Oct. 2014.

Feasibility Analysis - Materials ● Polycarbonate (PC) o Several buying options o Variety of readily available sizes o Low cost ● Polymethylpentene (PMP) o Very limited buying options o Sparse size options o Far more expensive than PC

Feasibility Analysis-Budget

FMEA

FMEA Cont.

Risk Assessment Lowered Risks ● Risk 8-Sensor Knowledge ● Risk 17-Media contamination during microscope viewing Increased Risks ● Risk 18-Media contamination during exchange “Still at Large” risks ● Risk 15 and 16-Shear stress measurement

Project Plan: MSD-I

Deliverables ● Proof of Concept o Further Engineering Analysis o Simulations/3D Models o Prototyping of Subsystems ● Consultation with Material Experts o Dr. Man o Dr. Gaborski o Mr. Hanzlik

Questions, Comments, or Concerns? Thank you!

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