Flow Culture System Multi-Disciplinary Senior Design System Design - - PowerPoint PPT Presentation

Flow Culture System

October 2, 2014

Collin Burkhart Katelyn Busse Michelle Garofalo Robert Repetti Morgan Stoessel Sarah Tran

Multi-Disciplinary Senior Design System Design Review

• CRs and ERs
• Functional

Decomposition

• Morphological Chart
• Benchmarking
• Concept

Generation/Pugh Analysis

• Turning Point/Concept

Selection

Agenda

• System Architecture
• Alternative Concepts
• Feasibility Analysis
• FMEA
• Risk Assessment
• Project Plan
• Deliverables
• Questions

Essential Customer Requirements

Essential Engineering Requirements

Functional Decomposition

View Cells

Culture Cells

Control Environment

Morphological Chart-1

Morphological Chart-2

Morphological Chart-3

Benchmarking - Whole System

Benchmarking - Whole System

Benchmarking - Pumps

Benchmarking - Materials

Benchmarking

Shear Measurement and Control Requirements

Shear Range: 0-20 dynes/cm2 = 0-2.9X10-4 psi Flow Range: 0-5.34 mL/second System Temperature: 25-37℃ Autoclave Temperature: Max 121℃

Strain Gauge Load Transducer Conversion Table Can it handle autoclave and system temperatures? No Yes N/A Can it measure a low force value? No (0.2 psi) No (0.1 psi) Yes (Flow Sensor) Can a display be made? Yes Yes Yes Is it cost feasible? <500 Yes ($160) No ($500) Yes (Free)

Concept Generation-Pugh Analysis

Turning Point

Concept Selection

Pump Cell Chamber Media Reservoir Electricity Flow Rate Cells Microscopes Alternate Reservoir Media Media Color Heat CO2 Water Pan

Architecture Color Structure Energy Information Materials

Incubator

Filter

System Architecture

Alternative Cell Viewing Concepts

Keep Attached for Viewing

• Have to move

microscope next to incubator

• Extra length of hose to

take out of incubator and set on microscope

• Carry total system on a

tray

Detach from System for Viewing

• Increase risk for

contamination

• Close off chamber

○ clamps on tubing (2

• n each side)

○ Valves ○ Caps (like flasks currently use)

Alternative Subsystem Concepts

Media Circulation System

Feasibility Analysis-Pumps

Peristaltic Pump Syringe Pump

Feasibility Analysis-Shear Stress/Flow Rates

• Values used for calculation
• μ=0.78x10^-3 Ns/m^2
• b=0.06m
• a=0.0125m
• T=2 N/m^2
• Flow Rate
• .016 L/s
• Dependent on Chamber Size

T=shear stress μ=viscosity of the liquid Q=flow rate b=channel width a=½ channel height

1. Kojima, Naoya, Mitsuru Shiota, Yoshito Sadahira, Kazuko Handa, and Sen-itiroh Hakomori. "Cell Adhesion in a Dynamic Flow System Compared to Static System."The Journal of Biological Chemistry 267.24 (1992): 17264-7270. Web. 1 Oct. 2014. 2. Wang, Chong, Hao Lu, and Martin Alexander Schwartz. "A Novel in Vitro Flow System for Changing Flow Direction on Endothelial Cells." Journal of Biomechanics 45.7 (2012): 1212-218. ELSEVIER. Web. 1 Oct. 2014.

Feasibility Analysis - Materials

• Polycarbonate (PC)
• Several buying options
• Variety of readily available sizes
• Low cost
• Polymethylpentene (PMP)
• Very limited buying options
• Sparse size options
• Far more expensive than PC

Feasibility Analysis-Budget

FMEA

FMEA Cont.

Risk Assessment

Lowered Risks

• Risk 8-Sensor Knowledge
• Risk 17-Media

contamination during microscope viewing Increased Risks

• Risk 18-Media

contamination during exchange “Still at Large” risks

• Risk 15 and 16-Shear

stress measurement

Project Plan: MSD-I

Deliverables

• Proof of Concept
• Further Engineering Analysis
• Simulations/3D Models
• Prototyping of Subsystems
• Consultation with Material Experts
• Dr. Man
• Dr. Gaborski
• Mr. Hanzlik

Questions, Comments,

• r

Concerns? Thank you!

Back-Up Slides

House of Quality: https://edge.rit.edu/edge/P15080/public/Problem%20Definition%20Documents/Additional%20Documents

Back Up Slides

Back Up Slides