FIRST RECORD OF WINTER PLANKTONIC MICROBIAL COMMUNITIES IN SOME - - PowerPoint PPT Presentation

▶
first record of winter
SMART_READER_LITE
LIVE PREVIEW

FIRST RECORD OF WINTER PLANKTONIC MICROBIAL COMMUNITIES IN SOME - - PowerPoint PPT Presentation

Oct 12, 2023 158 likes •369 views

FIRST RECORD OF WINTER PLANKTONIC MICROBIAL COMMUNITIES IN SOME SPECIFIC AQUATIC HABITATS RAMGANESH SELVARAJAN 1 , GERGELY KRETT 2 , ISTVN MTH 3 , LAURA JURECSKA 4 , ATTILA SZAB 2 , KROLY MRIALIGETI 2 , TAMS FELFLDI 2 1 Department

slide-1
SLIDE 1

FIRST RECORD OF WINTER PLANKTONIC MICROBIAL COMMUNITIES IN SOME SPECIFIC AQUATIC HABITATS

RAMGANESH SELVARAJAN1, GERGELY KRETT2, ISTVÁN MÁTHÉ3, LAURA JURECSKA4, ATTILA SZABÓ2, KÁROLY MÁRIALIGETI2, TAMÁS FELFÖLDI2

1Department of Plant Biology and Plant Biotechnology, University of Madras, Chennai, India 2Department of Microbiology, Faculty of Science, Eötvös Loránd University, Budapest, Hungary; 3Department of Bioengineering, Sapientia Hungarian University of Transylvania, Miercurea Ciuc, Romania 4Cooperative Research Center for Environmental Science, Faculty of Science, Eötvös Loránd University,

Budapest, Hungary

slide-2
SLIDE 2

Life during winter is different…

Lower temperature Surface ice cover (and snowpacks): reduces underwater light intensity reduces the exchange of heat, gases, particles between the atmosphere and the water coloumn prevents the mixing effect of wind…. etc. + Sampling sites are not easily accessible

slide-3
SLIDE 3

…and additionally specific habitats

slide-4
SLIDE 4

Aims

to explore the planktonic microbial communities of these specific aquatic habitats (composition of microbial communities under the ice)

Methods

abundance of microorganisms: (epifluorescence) microscopy taxonomic composition of communities: denaturing gradient gel electrophoresis and sequence analysis (16S ribosomal RNA gene) physico-chemical analyses

slide-5
SLIDE 5

General and measured characteristics of sampling sites

7-9th February 2013

slide-6
SLIDE 6

Lake Ursu (Bear Lake)

slide-7
SLIDE 7

Stratification in Lake Ursu

slide-8
SLIDE 8

Upper 3.5 m layer of L. Ursu

Cryptomonas sp. small green algae

Pt colour - chromophoric dissolved organic matter PAR - photosynthetically active radiation P-Euk - picoeukaryotic green algae P-Cya - picocyanobacteria

slide-9
SLIDE 9

Upper 3.5 m layer of L. Ursu

Prosthecochloris vibrioformis

slide-10
SLIDE 10

Saline lakes

BACTERIA ARCHAEA CYANOBACTERIA + PLASTIDS heterotrophic, marine and/or psychrophilic prokaryotes: L. Ursu: Albidiferax sp. (B7), Polaromonas glacialis (B8), halobacteria (A6-A9); L. Verde: Marinobacter psychrophilus (B15); L. Rosu: halobacteria (A10, A11) photoautotrophs: L. Ursu: Prosthecochloris vibrioformis (green sulphur bacterium, B9- B12), Cryptomonas sp. (microscopy); L. Rosu and L. Verde: Dunaliella sp. (microscopy)

slide-11
SLIDE 11

General and measured characteristics of sampling sites

7-9th February 2013

slide-12
SLIDE 12

Lake St. Ana

slide-13
SLIDE 13

Depth profiles of deep lakes

  • L. St. Ana L. Ursu
slide-14
SLIDE 14

Mohos peat bog lake

slide-15
SLIDE 15

Apor lányok feredője

pH 2.4-4.8 NH4

+ (mg/L) 1.4-2.2

NO2

  • (mg/L) 0.03-0.05

NO3

  • (mg/L) 7.5-8.2

PO4

3- (mg/L) 0.6-6.0

Fe (mg/L) 0.5-1.2 SO4

2- (mg/L) 327-820

TOC (mg/L) 14.3-16.5 IC (mg/L) 384-422

slide-16
SLIDE 16
  • L. St. Ana

Euglena proxima Astasia sp. Rhodomonas sp. Chlamydomonas microscopica Chl-a 81.3 µg L-1 pH 3.9

Mohos

Cryptomonas sp. Peridinium inconspicum Chl-a 1.1-1.9 µg L-1 pH 5.2-5.5 TN <1 mg L-1, PO4

3- <0.05 mg L-1

slide-17
SLIDE 17

2.2-32 x 105 cells/mL

Apor lányok feredője

slide-18
SLIDE 18
  • L. St. Ana,

Mohos, Apor

CYANOBACTERIA BACTERIA ARCHAEA + PLASTIDS heterotrophic prokaryotes: L. St. Ana: Prosthecobacter sp. (B1), Gluconobacter sp. (B2); Mohos: Polynucleobacter necessarius (B3); Apor: Metallibacterium schefferi (B5) photoautotrophs: L. St. Ana: Cryptomonas sp. (C1-C4, microscopy), Peridinium inconspicum (microscopy); Mohos: Euglena proxima, Astasia sp., Rhodomonas sp., Chlamydomonas microscopica (microscopy), Parachlorella sp. (C7, C8)

  • thers: Mohos: Methanosaeta sp. (methanogenic archaeon, A2, A3);

Apor: Acidithiobacillus ferrooxidans (acidophilic sulphur-oxidizing bacterium, B6), Ferroplasma acidiphilum (acidophilic iron-oxidizing archaeon, A4)

slide-19
SLIDE 19

Conclusions

Although many of the studied environments were extreme habitats regarding some or several parameters (low pH, high salinity, low light intensity etc.), microbial communities in many cases were abundant and/or diverse with the presence of previously unidentified taxa. Even phototrophic microbes may form dense populations under the ice, as it was observed in the case of the green sulphur bacterium Prosthecochloris in the stratified saline lake.

slide-20
SLIDE 20

Acknowlegements

Phytoplankton studies

Lajos Vörös, Boglárka Somogyi, Balázs Németh

Sample collection and processing

Szabolcs Szilveszter, Zsuzsanna Nagymáté, Róbert Fikó, Mihály Koncz, Zsolt Gyula Keresztes, Csaba Romsics, Mihály Braun, János Korponai This work was partially supported by the grants from the Hungarian Scientific Research Fund (OTKA PD 105407), from the Romanian National Authority for Scientific Research CNCS-UEFISCDI (PN-II-RU-TE-2012-3- 0319) and from the Balassi Institute - Hungarian Scholarship Board.