EFFECT OF ALKALINE PEROXIDE PRETREATMENT ON FIBRE COMPOSITION OF - - PowerPoint PPT Presentation

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EFFECT OF ALKALINE PEROXIDE PRETREATMENT ON FIBRE COMPOSITION OF - - PowerPoint PPT Presentation

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EFFECT OF ALKALINE PEROXIDE PRETREATMENT ON FIBRE COMPOSITION OF VARIOUS LIGNOCELLULOSIC RESIDUES Cristina Marzo, J. lvarez, A.B. Daz, I. Caro, A. Blandino University of Cdiz (Spain) e-mail: cristina.marzo@uca.es I NTRODUCTION

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EFFECT OF ALKALINE PEROXIDE PRETREATMENT ON FIBRE COMPOSITION OF VARIOUS LIGNOCELLULOSIC RESIDUES

Cristina Marzo, J. Álvarez, A.B. Díaz, I. Caro, A. Blandino

University of Cádiz (Spain)

e-mail: cristina.marzo@uca.es

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INTRODUCTION

University of Cádiz (Spain) e-mail: cristina.marzo@uca.es 2

Exhausted sugar beet cossettes (ESBC) Rice husk (RH) Wheat straw (WS) Sunflower stalk (SS) Fuels Fuels Polyhydroxyalkanoates (PHAs) Organic acids Low-Cost Abundant

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INTRODUCTION

Lignocellulosic biomass Pretreatment Enzymatic hydrolysis Fermentation

University of Cádiz (Spain) e-mail: cristina.marzo@uca.es 3

Biotechnological process

Alkaline hydrogen peroxide pretreatment The aim is to break down the recalcitrant structure of lignocellulose to make cellulose and hemicellulose more accessible to the enzymes

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INTRODUCTION

This pretreatment is an oxidative process which could significantly improve biomass digestibility.

It selectively removes lignin and deconstructs the cell walls.

University of Cádiz (Spain) e-mail: cristina.marzo@uca.es 4

Alkaline hydrogen peroxide pretreatment

Advantages

Disadvantages

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MATERIAL AND METHODS

UNIVERSITY OF CÁDIZ (SPAIN) E-MAIL: CRISTINA.MARZO@UCA.ES 5

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MATERIAL AND METHODS

University of Cádiz (Spain) e-mail: cristina.marzo@uca.es 6

Exhausted sugar beet cossettes (ESBC) Rice husk (RH) Wheat straw (WS) Sunflower stalk (SS)

Particle size

  • 0 – 0.5 mm
  • 0.5 – 1 mm
  • 1 – 1.5 mm
  • 1.5 – 2 mm

Particle size

  • 0.5 – 1 mm

 All the solids were milled and sieved

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MATERIAL AND METHODS

Alkaline hydrogen peroxide pretreatment

University of Cádiz (Spain) e-mail: cristina.marzo@uca.es 7

Hydrogen peroxide at 4 % (w/v) Solid Incubation 30ºC – 24h Filtration Washing Neutral pH Drying 40ºC - 24h pH 11.5 (NaOH)

Whatman No. 1 filter paper

1:20 (w/v)

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MATERIAL AND METHODS. FIBRE COMPOSITIONAL ANALYSIS

The determination of acid detergent fibre (ADF) and acid detergent lignin (ADL) according to EN ISO 13906:2008

The determination of amylase treated neutral detergent fibre (NDF) according to AOAC 2002:04/ISO 16472:2006.

University of Cádiz (Spain) e-mail: cristina.marzo@uca.es 8

Code Sample fraction Compliant substances Denomination

A removed with acetone Fats, oils, wax fats B+C removed with neutral detergent Proteins, enzymes, pectins, soluble salts, etc. Salts and no cellulosic B no calcined removed soluble salts Salts C calcined removed rest of removable material no saline no cellulosic D removed with acid detergent Hemicellulose, etc. Hemicellulose E removed with concentrated acid Cellulose, soluble lignin Cellulose D+E removed with acid hemicellulose, cellulose and soluble lignin Cellulose and Hemicellulose F calcined not removed insoluble lignin, etc. Lignin G not calcined and not removed insoluble salts, minerals, etc. Minerals B+G total no calcined T

  • tal salts

Salts and minerals

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RESULTS AND DISCUSSION

UNIVERSITY OF CÁDIZ (SPAIN) E-MAIL: CRISTINA.MARZO@UCA.ES 9

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RESULTS AND DISCUSSION. Influence on the solid particle size

University of Cádiz (Spain) e-mail: cristina.marzo@uca.es 10

Before AHP pretreatment After AHP pretreatment AHP pre-treatment was applied on milled rice husk and four different ranges of size were assayed

  • 0 – 0.5 mm
  • 0.5 – 1 mm
  • 1 – 1.5 mm
  • 1.5 – 2 mm

The average weight losses produced after AHP peroxide pre-treatment was 25.31 ± 0.38 % Fibre composition of rice husk

10 20 30 40 50 60 70 Composition (%)

0,0 - 0,5 mm 0,5 -1,0 mm 1,0 - 1,5 mm 1,5 -2,0 mm

10 20 30 40 50 60 70 Composition (%)

0‐0,5 0,5‐1

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RESULTS AND DISCUSSION. Effect on different agro-industrial residues

University of Cádiz (Spain) e-mail: cristina.marzo@uca.es 11

Fibre composition before AHP Fibre composition after AHP

10 20 30 40 50 60 70 Composition (%)

Sunflowers stalks Wheat straw Exhausted sugar beet cossettes Rice husks

10 20 30 40 50 60 70 Composition (%)

Sunflowers stalks Wheat straw Exhausted sugar beet cossettes Rice husks

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10 20 30 40 50 60 70 Composition (%)

Rice husks

Before After

RESULTS AND DISCUSSION. Effect on different agro-industrial residues

University of Cádiz (Spain) e-mail: cristina.marzo@uca.es 12

10 20 30 40 50 60 70 Composition (%)

Sunflowers stalks

Before After 10 20 30 40 50 60 70 Composition (%)

Wheat straw

Before After 10 20 30 40 50 60 70 Composition (%)

Exhausted sugar beet cossettes

Before After

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CONCLUSIONS

UNIVERSITY OF CÁDIZ (SPAIN) E-MAIL: CRISTINA.MARZO@UCA.ES 13

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CONCLUSIONS

Lignocellulosic biomass is a potential raw material for the production of added-value products through fermentation

  • f monomeric sugars. Nevertheless, a previous pretreatment stage is needed to make the polymers more accessible to

the enzymes in the hydrolysis step producing the fermentable sugars

University of Cádiz (Spain) e-mail: cristina.marzo@uca.es 14

Efficient pretreatment to remove a significant amount of lignin

Cellulose fraction was not affected achieving its concentration

Higher concentrations of hydrolysable polymers were attained; higher hydrolysis yields.

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EFFECT OF ALKALINE PEROXIDE PRETREATMENT ON FIBRE COMPOSITION OF VARIOUS LIGNOCELLULOSIC RESIDUES

Cristina Marzo, J. Álvarez, A.B. Díaz, I. Caro, A. Blandino

University of Cádiz (Spain)

e-mail: cristina.marzo@uca.es