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HyServe

EC Blue for Water Testing

HyServe

EC Blue for water testing

Can you detect contaminant (bacteria) from water without color?

HyServe

• EC Blue was introduced in 1998, and being an official water test method

in Japan approved by JWWA (2001).

• There are 3 different presentations (packages) prepared for E. coli and

coliform detection from water. EC Blue is “Culture Medium”.

• For MPN : EC Blue 10 (in 10mL non-self fluorescent tube)

and new MPN device

• For detection: EC Blue 100 (in 100mL non-self fluorescent bottle)
• For detection: EC Blue 100P (in a sachet for 100mL)

EC Blue for water testing (1)

Product profile, EC Blue

EC Blue 10 EC Blue 100 EC Blue 100P

HyServe Medium composition of EC Blue (17.4g for 1 L)

EC Blue for water testing (2)

0.1 g 4MUG 0.1 g X-GAL Enzyme substrate 0.1 g IPTG (Isopropyl-beta-D(-)-Thiogalactopyranoside) 1.0 g Potassium Nitrate 1.0 g Sodium Pyruvate 0.1 g Sodium Lauryl Sulfate 4.0 g Dipotassium Hydrogen Phosphate 1.0 g Monopotassium Phosphate 5.0 g Sodium Chloride 5.0 g Peptone Base medium

HyServe EC Blue: Feature and benefit

EC Blue for water testing (3)

Confirmation or re-test are not necessary and it could be save those costs. Easy to judge and detect E. coli by fluorescence (APL is non-self fluorescent material). Do not need to wait till medium to be room temperature (save preparation time). Can be stored at room temperature (under 30 C˚) Easy and convenient to control lab workflow. Shorter incubation and detection time compared with other products (18-24hours). Save inventory and purchasing cost Long shelf life (1 year after manufacturing) Conservation of environment (APL does not produce dioxin and other toxic gases). Easy to dispose after autoclaving (bottle and tube are made from APL).

Benefit Feature

HyServe Interpretation

EC Blue for water testing (4)

Coliform Positive

• E. coli Positive

Color change of medium (Green- Blue) might be Coliform positive Observation of fluorescence might be E. coli positive

HyServe Product Comparison vs. Colilert (IDEXX)

EC Blue for water testing (5)

Glass tube APL tube (easy to dispose) Package (for MPN) Cool temp Room temp (under 30 C˚) Storage condition

• E. coli, coliform only

Wider than Colilert (E. coli, coliform, other contaminant bacteria) Recovery Difficult to confirm (from Yellow background) Easy to confirm from Green – Blue background Fluorescent confirmation Ammonium sulfate, other ONPG / MUG Peptone, other X-GAL / MUG Composition of the product (Enzyme substrate used) Colilert IDEXX EC Blue Nissui Pharmaceutical

Product / manufacturer Comparison item

HyServe

• Market situation and the future possibility of EC Blue:

1. Market situation:

• Market value (US$): Europe – 10mil. / US – 9mil. / Japan – 4mil.
• Competitors: less competition. IDEXX and Merck are the major players.

IDEXX (Colilert) has over 80% of market share world wide.

• IDEXX has many official approvals for Colilert.

2. Future possibility of EC Blue:

• 100mL presentation has introduced in December 2003 (100P).
• EC Blue also will apply for official approvals

(if necessary, EPA/APHA/FDA/AOAC etc.).

• new innovative MPN device.

EC Blue for water testing (6)

HyServe

• Products Package Code

EC Blue 100P 100 pcs. 05591 EC Blue 100 80 bottles 05593 EC Blue 10 150 tubes 05613 (Shelf life / Storage = 1 year / Dark and RT) EC Blue Comparator 1 bottle 05617

EC Blue for water testing

EC Blue 100P EC Blue 100 EC Blue 10