eDNA sampling - complementing to port sampling Brofjorden port, - - PowerPoint PPT Presentation

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eDNA sampling - complementing to port sampling Brofjorden port, - - PowerPoint PPT Presentation

Jan 30, 2023 34 likes •106 views

eDNA sampling - complementing to port sampling Brofjorden port, Lysekil 2016 Marine Monitoring and Gothenburg university Erland Lettevall erland.lettevall@havochvatten.se Method parallel sampling plankton and benthos Organisms manually

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eDNA sampling - complementing to port sampling

Brofjorden port, Lysekil 2016 Marine Monitoring and Gothenburg university

Erland Lettevall erland.lettevall@havochvatten.se

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Method – parallel sampling plankton and benthos

  • Organisms manually sorted to identify all specimens to species
  • r higher taxonomic level
  • DNA is extracted from bulk samples
  • DNA metabarcoding
  • DNA was sequenced using next generation sequencing

techniques – Illumina

  • Matched against taxonomic reference sequences from public

archives and target species databases (GenBank, BOLD).

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Results

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Method species identification

  • No. of

taxa Species level %

  • No. of

IAS/NIS

  • No. of

Helcom/ Ospar IAS species Traditional 212 131 62% 4 2Mnemiopsis leydi, Crassostrea gigas eDNA 153 119 77% 5 5 Acartia tonsa, Crassostrea gigas, Crepidula fornicata, Amphibalanus improvisus and Caprella mutica. (Mnemiopsis leydi)

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Results 2

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Methods traditional eDNA Settling panel 75% 56% Benthic 82% 64% Plankton 9% 66%

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Conclusion: Traditional methods

  • vs. eDNA
  • Traditional methods
  • more species in general
  • DNA based methods

1. more IAS (higher specificity) 2. invasive species more often (higher sensitivity) 3. was able to detect invasive species among the plankton (early warning).

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Monitoring program reports

2017-11-17 Naturvårdsverket | Swedish Environmental Protection Agency 6

  • Helcom/Ospar JHP

Test and evaluation of port survey 2014 (Swe.) and 2016 (Eng. summary

  • Report on eDNA

Will be published in 1st quater

  • f 2018

(Eng. summary)

https://www.havochvatten.se/hav/uppdrag-- kontakt/publikationer/publikationer/2016-09-21-metoder-for-overvakning-av- frammande-arter.html https://www.havochvatten.se/hav/uppdrag-- kontakt/publikationer/publikationer/2017-06-07-test-och-utvardering-av-ny-

  • vervakning-av-frammande-arter-i-hamnar-och-utsatta-omraden.html
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Rapid assessment – test and evaluation in Swedish waters 2017

  • The Skagerrak
  • Brofjorden, Lysekil petroleum port
  • The Baltic Sea
  • Bullandö, marina off Stockholm
  • Nynäshamn port
  • Nynäshamn marina

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Report on eRAS Will be published in summer 2018