Dr M F Ishiyaku Researcher Making Crosses Inside Containment - PowerPoint PPT Presentation

Dr M F Ishiyaku

Researcher Making Crosses Inside Containment Facility

Hybrid Pod of 709A & 499

Marker for Selecting presence of Bt in leaf samples

Multilocation Evaluations Conducted at Bakura, Minjibir & Samaru (2013-2015) Selected most Elite lines IT97KT & IT93KT NTO, EIA Trials

FARMER-MANAGED TRIAL IN CFF Dr M F Ishiyaku

FARMER-MANAGED TRIAL ❑ LOCATIONS: SAMARU, BAKURA & MINJIBIR ❑ FARMERS: SIX IN EACH LOCATION ❑ EACH FARMER: TESTS LOCAL, TRANSGENIC & NON TRANSGENIC ❑ EACH FARMER APPLY TWO INSECTICIDE SPRAYS ONLY ❑ EACH FARMER MANAGES CROP THEIR WAY Dr M F Ishiyaku

Dr M F Ishiyaku

Dr M F Ishiyaku

Dr M F Ishiyaku

Product Development: farmers-managed confined trials 6 farmers per sites Conducted the trial in RCBD Farmers constitute the blocks

Product Development: farmers-managed confined trials 6 farmers per sites Conducted the trial in RCBD Farmers constitute the blocks

Product Development: farmers-managed confined trials Farmers’ ranking of the Cowpea varieties in Minjibir, Nigeria, 2017; percentage of farmers per rank and per variety Farmers: n=36 Varieties Ranking scale (1-3): 1=most preferred Rank IT97KT Sampe Local 3=least preferred (check) 1 61.1 27.8 11.1 2 33.3 47.2 19.4 3 5.6 25.0 69.4

Product Development: farmers-managed confined trials Farmers’ ranking of the Cowpea varieties in Zaria, Nigeria, 2017; percentage of farmers per rank and per variety Varieties Farmers: n=36 Ranking scale (1-3): 1=most preferred, Rank IT97KT Sampea Local 3=least preferred (check) 1 63.9 22.2 13.9 2 36.1 38.9 25.0 3 0.0 38.9 61.1

Product Development: farmers-managed confined trials Farmers’ ranking of the Cowpea varieties in Bakura, Nigeria, 2017; percentage of farmers per rank and per variety Varieties Farmers: n=36 Rank IT97KT Sampea Local Ranking scale (1-3): 1=most preferred (check) 3=least preferred 1 63.8 22.2 16.7 2 30.6 41.7 25.0 3 5.6 36.1 58.3

Endorsed the PBR test lines ▪ Relative high yield under fewer sprays ▪ Earliness ▪ Quality ▪ Relative low cost of production

Multiplication: Nucleus Material of Purified Seeds

IS PBR COWPEA SAFE TO THE ENVIRONMENT? SAFE AS FOOD/FEED

Distribution of non-target insects on Bt, non-Bt and wild cowpea under insecticide sprays and no sprays tested in Zaria, 2014 Line Insects Treatment Bees Locust Maruca L L Spittle Beetle Beetle bug 1 2 Bt Insectic 7.3 2.3 0.8 0.3 0.3 0.3 Non Bt Insectic 9.5 0.5 1.0 0.8 0.3 0.3 Wild Insectic 6.3 0.5 0.3 0.5 0.5 3.0 Bt Water 8.8 2.3 0.3 0.8 0 0.3 Non Bt Water 10.3 1.3 2.0 0,3 0 16.5 Wild Water 6.8 1.0 0.5 0.8 0.8 34.4

Fallow for 6 years Harvested and cleared portion planted to Bt Cowpea Dr M F Ishiyaku

Proximate: Grains, Leaves and fodder ▪ No significant difference between GM and non-GM (P >0.1) Mineral Composition ▪ No significant difference between GM and non-GM (P> 0.1) Phytic acid ▪ No significant difference between GM and non-GM (P> 0.7) ▪ The gene is not similar to any gene(s) known to cause allergenicity ▪ Gene has long history of safe use in many crops (over 20 years)

At least 20% yield increase per hectare. Translates to forty-eight billion naira (N48,000, 000,000.0) annually @ N120,000.0 per tonne

Reduction of Insecticide spray requirement from 6-8 litre per hectare to 2-3 litre per hectare @ N1200.0 per litre = N5400.0 reduction in production cost per hectare planted with PBR Cowpea, if of the 3million hectares 1million is planted to PBR Cowpea the savings from cost of insecticide is sixteen billion and two hundred million naira (N16,200, 000,000.0) annually

Healthier environment from reduced insecticide spray-Beneficial Insects and other organisms in the ecosystem Reduced human health risk-more productive farming population

Applications-Accreditation of Facilities Application-Confinement of transgene Application-Containment-Introgression Application-Multilocation Confinement Application-Farmer Confined Evaluation Application-Deregulation = Release

Development of farmer-type Bt Cowpea accomplished in full compliance with regulatory requirement The Bt gene bred into Cowpea is very efficacious against Maruca Pod borer Farmer assessment of the new PBR variety reveals endorsement among participating farmers due to its benefits Over 48 Billion naira gain will be made by planting 1 out of every 3 hectares of cowpea as Bt Cowpea in Nigeria

Thank you!!!