DNA barcoding of South African red seaweed (Rhodophyta) Presented - PowerPoint PPT Presentation

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DNA barcoding of South African red seaweed (Rhodophyta) Presented by Jamie Julies Stellenbosch University Genetics Department, Molecular Breeding and Biodiversity group 14 August 2018 Prof. Rouvay Roodt-Wilding Prof. John Bolton Prof.

DNA barcoding of South African red seaweed (Rhodophyta) Presented by Jamie Julies Stellenbosch University Genetics Department, Molecular Breeding and Biodiversity group 14 August 2018 Prof. Rouvay Roodt-Wilding Prof. John Bolton Prof. Robert Anderson
Introduction »South Africa is renowned for its diverse seaweed flora » Port Alfred has a high species diversity and endemicity » Rhodophyta is of particular importance » Limited taxonomic classification of species J.J. Bolton & H. Stegenga, 2002 2
Aim and Objectives Use DNA barcoding as a tool to characterise and identify red seaweed species occurring in the Port Alfred region 1. Assessing three gene regions 2. Increasing the barcoding reference database 3. Potentially identifying new species 3
What has been done? LSU COI-5P rbc L (~ <2000 bp) (~ 664 bp) (~ 785 bp) » Sampling of 132 samples 2% 12% [VAL » DNA isolation via a modified UE] CTAB method (Clarke, 2009) [VAL 88% 98% » Marker amplification profiles UE] (Saunders and Moore, 2013) Success Fail 4
Future aspects » Bi-directional sequencing of gene products » Sequence analysis » Data analysis » Species pages 5
Acknowledgements 6

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