De Defense Threat Reduction Agency (D (DTRA) Technology Man - - PowerPoint PPT Presentation

Sc Scie ientif ific ic Development Fell llowship Program: Applic icatio ion of f modern dia iagnostic ic techniques for r dia iagnosis of f an anim imal l in infectio ious dis iseases in in Cornell ll Univ iversity, USA

Dr

• Dr. Bo

Bozorboy Elm lmurodov

• Mr. Ja

Jasurbek Yulch lchie iev

Ithaca, New York - 2018

De Defense Threat Reduction Agency (D (DTRA) Technology Man anagement Company (TM (TMC)

Bo Bozorb rboy Elmurodov - MS, S, PhD, , Doctor of Veterin inary ry Sc Scie ience

❖ Depute Director of Scientific Research Institute of

Veterinary (SRIV)

❖Expertise: Bacteriology & Epidemiology ❖Major: Study of bacterial diseases in agricultural

animals; involvement in the vaccine production process against bacterial diseases of animals

❖Current

research topic: Study

• f

mixed bacterial diseases in small ruminants

Jas Jasurbek Yulch lchie iev - MS, S, Scien ientif ific ic-researcher, Sa Samarkand veterin inary ry medic icin ine ins instit itute ❖Assistant Professor of Department of Veterinary Surgery,

Veterinary Prevention and Treatment Faculty, Samarkand Institute of Veterinary Medicine.

❖Major: Study of surgical treatment in agricultural animals

and oncology in small animals (dogs)

❖Current

research topic: Study

• f

Etiology and Pathomorphology of breast tumors in dogs as well as working out of treatment of breast tumors in dogs;

Tim imelin ines and main in goals ls of Fell llowship program

• June 18 – July 13, 2018 (four-weeks of intensive

lecture-based and hands-on training)

• General overview of veterinary system in the US;
• Brief review of DVM curriculum and education system
• f veterinary universities in the US;
• Learn innovative methods of teaching process in the

veterinary colleges;

• Practicing

in new technology for surgical and therapeutic treatment of animals;

• Practicing in modern laboratory techniques (ELISA and

PCR) for diagnosis of animal diseases

Logistic ics provid ided by y TM TMC

• SVC fellows arrived

in the US

• June 15, 2018
• Orientation with

TMC Deputy manager (Dr. Jeein Chung)

• June 16, 2018
• SVC fellows arrived

at Cornell University

• June 17, 2018.
• Warmly received by
• Dr. Caroline Yancey

Learning Obje jectiv ives of Fell llowship ip Program

• Week – 1 (June 18-22,

2018)

• Week – 2 (June 25-29,

2018)

• Week – 3 (July 2-6, 2018)
• Week – 4 (July 9-13, 2018)

Cornell ll Departments

• Animal Health Diagnostic

Center

• Anatomic and Clinical

pathology Lab

• Bacteriology and microbiology
• Molecular diagnosis
• Virology
• Parasitology
• Serology

Faculty met (V (Vis isits of f fellows)

• Dairy farm and Quality Milk Production Service lab
• Institute dairy farm
• Institute Large animal hospital in Cornell vet school
• Poultry farms around Ithaca city
• Institute small animal hospital
• Institute research sheep farm
• “Lively run” traditional agro touristic goat dairy farm
• Institute equine park

Week – 1 Educatio ion system and DVM curr rric iculu lum of f Co Corn rnell ll Veteri rinary ry Co Coll llege

• Familiarizing

with education system and teaching methods as well as DVM

• Toured the main

Week – 1 Anim imal Healt lth Dia iagnostic Center

Week – 1 1 Main in tasks of Anim imal Healt lth Dia iagnostic ic Center

• Monitoring of domestic and wild animal for

zoonosis;

• Respond to disease outbreaks among animals;
• Establish diagnostic testing capabilities;
• Support disease surveillance and monitoring

programs;

• Assist analyzing and interpreting test results;
• Ability to detect diseases early that impact

animals.

Week – 1 Laboratory ry Bio iosecurit ity and Bio iosafety

• Biosecurity and

Biosafety concepts

• Farm-level Biosecurity
• Biological Safety

Cabinet: Working safely under the Hood

• Blood borne

pathogens Safety course

Week - 1 Vis isit to poult ltry ry farms

• The first farm have

250 000 laying hens and keep

• n

the ground.

• The second one have 1
• mln. laying hens and

keep in the cage.

• The

productivity

• f

this farm is very high. Average 97-98%

Week – 2 2 Anatomic ic and Clin linic ical l Pathology Laboratory ry

• Morphological describe

slides under microscope prepared from necropsy samples.

• Learning necropsy

methods

Week – 2 Anatomic ic and Clin linic ical l Pathology Laboratory ry

• Pathology rounds

(“Show and Tell”)

• To describing

necropsy results and preliminary diagnosis

Week - 2 Anatomic and Cli linical Pathology Laboratory ry

• Participating in sample receiving and slide preparation

process

• To prepare more than 200 microscope slides.
• They process slides by automated machines.

Week – 2 Visit isit to Dairy iry farm rm and Quali lity Mil ilk Productio ion Se Servi vice La Laboratory ry

• There are 1200 dairy

caws in farm.

• Average productivity
• f caws about 38-40 l

a day.

• Participating morning

milking process and tour all herd of farm.

• Full farm visit survey

and control mastitis

• f dairy caws.

Week - 2 Vis isit to in institute Large anim imal hospit ital

• Information an about

activity Large animal hospital.

• Rotation last year

student

• They practiced

treatment of cases.

• Describing patients and

answering questions

Week – 3 Vir irology and Serology la laboratorie ies

• Practicing different ELISA

techniques

• Performed ELISA test for

detection

• BVD (bovine viral diarrhea)

virus

• Salmonella enterica Dublin
• West Nile virus
• Feline toxoplasma
• Feline leukemia
• Canine leishmania
• Interpretation of ELISA test

results

Week - 3 Vis isit to in institute dairy ry farm

• High technological dairy farm
• Using teaching process
• clinical

and physical examination during the practical classes.

• To take blood samples also same

injection for sick dairy cow

Week - 3 Vis isit to in institute small l anim imal hospital

• Actual small animal

hospital in Cornell vet school.

• Very important for

promoting research and education

• Every year they

receive thousand dog and cat patients

Week - 4 Mole lecular Dia iagnosis is Laboratory ry

• Knowledge on PCR

techniques

• Learnt advantages of

PCR test

Week - 4 Mic icrobiology la laboratory

• Pipetting
• ELISA and PCR

techniques for bovine Salmonella and E.coli.

Week - 4 Parasitology Laboratory ry

• Many samples

collected from farms and wild animals.

• PCR tests
• Toxoplasmosis
• Tryponosomiasis
• Leishmaniosis

Week - 4 Visit isit to sh sheep farm rm; goat farm rm; ins instit itute of f equin ine park

• Research sheep

reproduction and nutrition sciences.

• Agro touristic goat farm
• Institute of equine park

What was dif ifferent?

• Application of ELISA and PCR techniques on a

regular basis.

• Automated

modern equipment and machines.

• Actively hands-on session for vet students.
• Modern technologies in livestock farms.
• Organizing veterinary faculty activity.

Using th this experience in in th the fu futu ture and benefits for SIV IVM/SRIV

• Using ELISA and PCR techniques in

teaching process and scientific research works.

• Initiate active involvement of vet students in hands-
• n session
• Assisting in awareness activity among livestock

farmers to introduce modern technologies/methods to get more benefit and safe product

• Preparing proposals for institute managers in order

to develop new regulations which lets institute staff to get benefit through chargeable vet services

Sugg ggestions for th the next xt SDFP fell llows

1. Choose one specific direction( Lab technique OR Epi concept) for the whole period of the training to learn in detail/deeper than multiple/wide directions. 2. Select simpler University for training purpose than high level University, it may help fellows to use learnt experiences/methods OR technologies in the future. 3. SDFP should consisted two part of trainings. First training part for a short period (4-weeks) and subsequently the second training part for a long period (4-5 month) 4. Establish short English courses for potential fellows before coming to the training to learn scientific terminologies and essential lab/epi procedures/tools as well as to better understand the whole concept of fellowship program 5. Establish international cooperation between host university and fellows institute to continue scientific collaboration/research in the future.

Acknowledgments and thanks

Thanks to:

• Dr. Caroline Yancey and her team
• Dr. Pilar Thompson
• Dr. Karyn Havas and her colleagues
• Dr. B.Nydam and his colleagues
• Dr. Jagne and her colleagues
• Administration of Cornell University
• TMC facilitators of US and Uzbekistan
• Administration of State Veterinary Committee of

Uzbekistan