De Defense Threat Reduction Agency (D (DTRA) Technology Man anagement Company (TM (TMC) Scie Sc ientif ific ic Development Fell llowship Program: Applic icatio ion of f modern dia iagnostic ic techniques for r dia iagnosis of f an anim imal l in infectio ious dis iseases in in Cornell ll Univ iversity, USA Dr Dr. Bo Bozorboy Elm lmurodov Mr. Ja Jasurbek Yulch lchie iev Ithaca, New York - 2018
Bo Bozorb rboy Elmurodov - MS, S, PhD, , Doctor of Veterin inary ry Sc Scie ience ❖ Depute Director of Scientific Research Institute of Veterinary (SRIV) ❖ Expertise: Bacteriology & Epidemiology ❖ Major: Study of bacterial diseases in agricultural animals; involvement in the vaccine production process against bacterial diseases of animals ❖ Current research topic: Study of mixed bacterial diseases in small ruminants
Jas Jasurbek Yulch lchie iev - MS, S, Scien ientif ific ic-researcher, Sa Samarkand veterin inary ry medic icin ine ins instit itute ❖ Assistant Professor of Department of Veterinary Surgery, Veterinary Prevention and Treatment Faculty, Samarkand Institute of Veterinary Medicine. ❖ Major: Study of surgical treatment in agricultural animals and oncology in small animals (dogs) ❖ Current research topic: Study of Etiology and Pathomorphology of breast tumors in dogs as well as working out of treatment of breast tumors in dogs;
Tim imelin ines and main in goals ls of Fell llowship program • June 18 – July 13, 2018 (four-weeks of intensive lecture-based and hands-on training) • General overview of veterinary system in the US; • Brief review of DVM curriculum and education system of veterinary universities in the US; • Learn innovative methods of teaching process in the veterinary colleges; • Practicing in new technology for surgical and therapeutic treatment of animals; • Practicing in modern laboratory techniques (ELISA and PCR) for diagnosis of animal diseases
Logistic ics provid ided by y TM TMC • SVC fellows arrived in the US • June 15, 2018 • Orientation with TMC Deputy manager (Dr. Jeein Chung) • June 16, 2018 • SVC fellows arrived at Cornell University • June 17, 2018. • Warmly received by Dr. Caroline Yancey
Learning Obje jectiv ives of Fell llowship ip Program • Week – 1 (June 18-22, 2018) • Week – 2 (June 25-29, 2018) • Week – 3 (July 2-6, 2018) • Week – 4 (July 9-13, 2018)
Cornell ll Departments • Animal Health Diagnostic Center • Anatomic and Clinical pathology Lab • Bacteriology and microbiology • Molecular diagnosis • Virology • Parasitology • Serology
Faculty met (V (Vis isits of f fellows) • Dairy farm and Quality Milk Production Service lab • Institute dairy farm • Institute Large animal hospital in Cornell vet school • Poultry farms around Ithaca city • Institute small animal hospital • Institute research sheep farm • “Lively run” traditional agro touristic goat dairy farm • Institute equine park
Week – 1 Educatio ion system and DVM curr rric iculu lum of f Co Corn rnell ll Veteri rinary ry Co Coll llege • Familiarizing with education system and teaching methods as well as DVM • Toured the main
Week – 1 Anim imal Healt lth Dia iagnostic Center
Week – 1 1 Main in tasks of Anim imal Healt lth Dia iagnostic ic Center • Monitoring of domestic and wild animal for zoonosis; • Respond to disease outbreaks among animals; • Establish diagnostic testing capabilities; • Support disease surveillance and monitoring programs; • Assist analyzing and interpreting test results; • Ability to detect diseases early that impact animals.
Week – 1 Laboratory ry Bio iosecurit ity and Bio iosafety • Biosecurity and Biosafety concepts • Farm-level Biosecurity • Biological Safety Cabinet: Working safely under the Hood • Blood borne pathogens Safety course
Week - 1 Vis isit to poult ltry ry farms • The first farm have 250 000 laying hens and keep on the ground. • The second one have 1 mln. laying hens and keep in the cage. • The productivity of this farm is very high. Average 97-98%
Week – 2 2 Anatomic ic and Clin linic ical l Pathology Laboratory ry • Morphological describe slides under microscope prepared from necropsy samples. • Learning necropsy methods
Week – 2 Anatomic ic and Clin linic ical l Pathology Laboratory ry • Pathology rounds (“Show and Tell”) • To describing necropsy results and preliminary diagnosis
Week - 2 Anatomic and Cli linical Pathology Laboratory ry • Participating in sample receiving and slide preparation process • To prepare more than 200 microscope slides. • They process slides by automated machines.
Week – 2 Visit isit to Dairy iry farm rm and Quali lity Mil ilk Productio ion Se Servi vice La Laboratory ry • There are 1200 dairy caws in farm. • Average productivity of caws about 38-40 l a day. • Participating morning milking process and tour all herd of farm. • Full farm visit survey and control mastitis of dairy caws.
Week - 2 Vis isit to in institute Large anim imal hospit ital • Information an about activity Large animal hospital. • Rotation last year student • They practiced treatment of cases. • Describing patients and answering questions
Week – 3 Vir irology and Serology la laboratorie ies • Practicing different ELISA techniques • Performed ELISA test for detection • BVD (bovine viral diarrhea) virus • Salmonella enterica Dublin • West Nile virus • Feline toxoplasma • Feline leukemia • Canine leishmania • Interpretation of ELISA test results
Week - 3 Vis isit to in institute dairy ry farm • High technological dairy farm • Using teaching process • clinical and physical examination during the practical classes. • To take blood samples also same injection for sick dairy cow
Week - 3 Vis isit to in institute small l anim imal hospital • Actual small animal hospital in Cornell vet school. • Very important for promoting research and education • Every year they receive thousand dog and cat patients
Week - 4 Mole lecular Dia iagnosis is Laboratory ry • Knowledge on PCR techniques • Learnt advantages of PCR test
Week - 4 Mic icrobiology la laboratory • Pipetting • ELISA and PCR techniques for bovine Salmonella and E.coli.
Week - 4 Parasitology Laboratory ry • Many samples collected from farms and wild animals. • PCR tests • Toxoplasmosis • Tryponosomiasis • Leishmaniosis
Week - 4 Visit isit to sh sheep farm rm; goat farm rm; ins instit itute of f equin ine park • Research sheep reproduction and nutrition sciences. • Agro touristic goat farm • Institute of equine park
What was dif ifferent? • Application of ELISA and PCR techniques on a regular basis. • Automated modern equipment and machines. • Actively hands-on session for vet students. • Modern technologies in livestock farms. • Organizing veterinary faculty activity .
Using th this experience in in th the fu futu ture and benefits for SIV IVM/SRIV • Using ELISA and PCR techniques in teaching process and scientific research works. • Initiate active involvement of vet students in hands- on session • Assisting in awareness activity among livestock farmers to introduce modern technologies/methods to get more benefit and safe product • Preparing proposals for institute managers in order to develop new regulations which lets institute staff to get benefit through chargeable vet services
Sugg ggestions for th the next xt SDFP fell llows 1. Choose one specific direction( Lab technique OR Epi concept) for the whole period of the training to learn in detail/deeper than multiple/wide directions. 2. Select simpler University for training purpose than high level University, it may help fellows to use learnt experiences/methods OR technologies in the future. 3. SDFP should consisted two part of trainings. First training part for a short period (4-weeks) and subsequently the second training part for a long period (4-5 month) 4. Establish short English courses for potential fellows before coming to the training to learn scientific terminologies and essential lab/epi procedures/tools as well as to better understand the whole concept of fellowship program 5. Establish international cooperation between host university and fellows institute to continue scientific collaboration/research in the future.
Acknowledgments and thanks Thanks to: • Dr. Caroline Yancey and her team • Dr. Pilar Thompson • Dr. Karyn Havas and her colleagues • Dr. B.Nydam and his colleagues • Dr. Jagne and her colleagues • Administration of Cornell University • TMC facilitators of US and Uzbekistan • Administration of State Veterinary Committee of Uzbekistan
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