Courage + Khazaka electronic GmbH Courage + Khazaka, Charit, et al. - PowerPoint PPT Presentation

SP18: Development of a fast non-invasive in vivo measurement of UVA-PF and SPF with new diffuse reflectance spectroscopy (DRS) device Wiora, Georg 2 ; Throm, C. 1 ; Reble, C. 2 ; Lademann, J. 1 ; Schanzer, S. 1 ; Kobylinski, S. 1 ; Karrer, H. 3 ; Rohr, M. 4 ; Kolbe, L. 5 ; Khazaka; G. 2 ; Meinke, M. C. 1 1 Charité - Universitätsmedizin Berlin, Berlin, Germany 2 Courage + Khazaka electronic GmbH, Cologne, Germany 3 Hans Karrer GmbH, Augsburg, Germany 4 Matthias Rohr, Institute Dr. Schrader, Holzminden, Germany 5 Beiersdorf, Hamburg, Germany Courage + Khazaka electronic GmbH Courage + Khazaka, Charité, et al.

State of the art: in vivo Sun Protection Factor according to ISO 24444 1. High dose UVA and UVB radiation on volunteers skin 2. Assessment of erythema 24h after exposure 3. MED = Minimal Erythema Dose http://www.schrader-institute.de/home/pruefung/haut-pruefung/sonnenschutz/ MED_with_Protection 4. SPF = MED_without_Protection 5. >10 subjects per product  FDA und EU call for non-invasive methods SP18: Non invasive SPF: Wiora, Throm, Lademann, Meinke et al., IFSCC 2019, Milano, October 2, 2019 Courage + Khazaka, Charité, et al. 1

New non-invasive LED-based DRS/HDRS system  DRS: in vivo Diffuse Reflectance Spectroscopy  System components:  Multi-lambda-LED light source  Customized fiber optics  Customized imaging spectrograph  Spectral result display for improved skin protection development  2 Operation modes: 1. pure in vivo 2. HDRS (Hybrid DRS): combination with in vitro [Ruvolo et al 2014], [Rohr et al 2018] SP18: Non invasive SPF: Wiora, Throm, Lademann, Meinke et al., IFSCC 2019, Milano, October 2, 2019 Courage + Khazaka, Charité, et al. 2

Pure in vivo UVA-PF results 40  9 products tested  N=10 volunteers 35  Aging correction included 30  Good correlation UVA-PF in vivo 25  Currently up to UVA-PF 30 20 (to be improved) 15 R² = 0,74 10 5 0 0 5 10 15 20 25 30 UVA-PF reference SP18: Non invasive SPF: Wiora, Throm, Lademann, Meinke et al., IFSCC 2019, Milano, October 2, 2019 Courage + Khazaka, Charité, et al. 3

HDRS results 140  HDRS [Ruvolo et al 2014], [Rohr et al 2018] 120  9 products tested SPF HDRS in vivo 100  N=10 volunteers  Aging correction included 80  Good correlation of HDRS 60 R² = 0,84 40 20 0 0 20 40 60 80 SPF reference ISO24444 SP18: Non invasive SPF: Wiora, Throm, Lademann, Meinke et al., IFSCC 2019, Milano, October 2, 2019 Courage + Khazaka, Charité, et al. 4

Summary  Non invasive technology  compact measurement system  Simple and safe application  Low effective UV-dose < 1/5 MED  Fast in vivo measurement. e.g.  4 products  on one volunteer   Less than 60 minutes (compared to 24h for ISO 24444)  Outlook  Participation in upcoming international ring study  Market introduction 2020 SP18: Non invasive SPF: Wiora, Throm, Lademann, Meinke et al., IFSCC 2019, Milano, October 2, 2019 Courage + Khazaka, Charité, et al. 5