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Courage + Khazaka, Charité, et al.

Courage + Khazaka electronic GmbH

SP18: Development of a fast non-invasive in vivo measurement of UVA-PF and SPF with new diffuse reflectance spectroscopy (DRS) device

Wiora, Georg2; Throm, C.1; Reble, C.2; Lademann, J.1; Schanzer, S.1; Kobylinski, S.1; Karrer, H.3; Rohr, M.4; Kolbe, L.5; Khazaka; G.2; Meinke,

• M. C.1

1 Charité - Universitätsmedizin Berlin, Berlin, Germany 2 Courage + Khazaka electronic GmbH, Cologne, Germany 3 Hans Karrer GmbH, Augsburg, Germany 4 Matthias Rohr, Institute Dr. Schrader, Holzminden, Germany 5 Beiersdorf, Hamburg, Germany

Courage + Khazaka, Charité, et al.

State of the art: in vivo Sun Protection Factor according to ISO 24444

1. High dose UVA and UVB radiation on volunteers skin 2. Assessment of erythema 24h after exposure 3. MED = Minimal Erythema Dose 4. SPF = MED_with_Protection MED_without_Protection 5. >10 subjects per product  FDA und EU call for non-invasive methods

http://www.schrader-institute.de/home/pruefung/haut-pruefung/sonnenschutz/

SP18: Non invasive SPF: Wiora, Throm, Lademann, Meinke et al., IFSCC 2019, Milano, October 2, 2019 1

Courage + Khazaka, Charité, et al.

New non-invasive LED-based DRS/HDRS system

• DRS: in vivo Diffuse Reflectance

Spectroscopy

• System components:
• Multi-lambda-LED light source
• Customized fiber optics
• Customized imaging spectrograph
• Spectral result display for improved skin

protection development

• 2 Operation modes:
• 1. pure in vivo
• 2. HDRS (Hybrid DRS): combination with in vitro

[Ruvolo et al 2014], [Rohr et al 2018]

SP18: Non invasive SPF: Wiora, Throm, Lademann, Meinke et al., IFSCC 2019, Milano, October 2, 2019 2

Courage + Khazaka, Charité, et al.

Pure in vivo UVA-PF results

SP18: Non invasive SPF: Wiora, Throm, Lademann, Meinke et al., IFSCC 2019, Milano, October 2, 2019 3

R² = 0,74

5 10 15 20 25 30 35 40 5 10 15 20 25 30 UVA-PF in vivo UVA-PF reference

• 9 products tested
• N=10 volunteers
• Aging correction included
• Good correlation
• Currently up to UVA-PF 30

(to be improved)

Courage + Khazaka, Charité, et al.

HDRS results

SP18: Non invasive SPF: Wiora, Throm, Lademann, Meinke et al., IFSCC 2019, Milano, October 2, 2019 4

R² = 0,84

20 40 60 80 100 120 140 20 40 60 80 SPF HDRS in vivo SPF reference ISO24444

• HDRS [Ruvolo et al 2014],

[Rohr et al 2018]

• 9 products tested
• N=10 volunteers
• Aging correction included
• Good correlation of HDRS

Courage + Khazaka, Charité, et al.

Summary

• Non invasive technology
• compact measurement system
• Simple and safe application
• Low effective UV-dose < 1/5 MED
• Fast in vivo measurement. e.g.
• 4 products
• on one volunteer
• Less than 60 minutes (compared to 24h for ISO 24444)
• Outlook
• Participation in upcoming international ring study
• Market introduction 2020

SP18: Non invasive SPF: Wiora, Throm, Lademann, Meinke et al., IFSCC 2019, Milano, October 2, 2019 5