COST LONG-TERM LIVE-CELL IMAGING PLATFORM FOR BIOMEDICAL RESEARCH - - PowerPoint PPT Presentation

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Mar 18, 2024 373 likes •543 views

DEVELOPING A COMPACT AND LOW- COST LONG-TERM LIVE-CELL IMAGING PLATFORM FOR BIOMEDICAL RESEARCH AND EDUCATION PURPOSE (SCBE06) CHUA YA XUAN MENTOR: DR PUI TZE SIAN INTRODUCTION LIVING THINGS ARE MADE UP OF CELLS AND CELLS ARE MADE UP OF

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DEVELOPING A COMPACT AND LOW- COST LONG-TERM LIVE-CELL IMAGING PLATFORM FOR BIOMEDICAL RESEARCH AND EDUCATION PURPOSE (SCBE06)

CHUA YA XUAN MENTOR: DR PUI TZE SIAN

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INTRODUCTION

LIVING THINGS ARE MADE UP OF CELLS AND CELLS ARE MADE UP OF

THOUSANDS OF MOLECULES THAT INTERACT WITH EACH OTHER.

IN ORDER TO MAKE NEW DISCOVERIES ABOUT THE CELLULAR DYNAMICS AND

DEVELOP DRUGS TO COMBAT DISEASES THAT ARISE FROM DYSFUNCTIONAL CELLS, SCIENTISTS ARE REQUIRED TO STUDY THE CONSTANTLY CHANGING PATTERNS OF THESE MOLECULES

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CURRENT METHODS

LONGITUDINAL STUDIES
TIME LAPSE MICROSCOPY

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LIMITATIONS

COSTLY CELLS ARE NOT IN OPTIMUM CONDITION BULKY

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AIM

MAKE USE OF 3D PRINTING, THE PROTOTYPING TECHNOLOGY, TO COME UP

WITH A COMPACT, LOW-COST AND EASY-TO-OPERATE LIVE-CELL IMAGING PLATFORM FOR BIOMEDICAL RESEARCH AND EDUCATION PURPOSE

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ENGINEERING GOALS

PHYSICAL FUCTIONAL HUMIDITY TEMPERATURE LOW-COST COMPACT

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METHODOLOGY

HOUSING-

3D MODEL OF THE TOP VIEW OF INCUBATOR 3D MODEL OF THE SIDE VIEW OF INCUBATOR

CAD software
Acrylic glass

Holder for CO2 sensor Temperature & humidity sensor

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METHODOLOGY

MICROCONTROLLER-
Arduino
pen-source

Integrated Development Environment

C++ coding language

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METHODOLOGY

INCUBATOR SYSTEM-

Top-view of the entire physical set-up Close-up view of incubator Close-up view of LED panel

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RESULTS

TEMPERATURE-

Graph of temperature against time to test how long the incubator takes to reach the ideal temperature of 28 ˚C

From a temperature of 24.4˚C, it took

85 minutes for the temperature to rise to 28˚C, the optimum temperature we aim to sustain throughout the experiment.

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RESULTS

TEMPERATURE-

Graph of temperature against time to test the stability of the incubator in maintaining the optimum temperature

Temperature only increases by 0.5˚C

in the next hour from 28˚C.

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RESULTS

TEMPERATURE-

𝑼 = 𝟐 𝑸 + 𝑪 + 𝑸𝟒 × 𝑫 + 𝑩 − 𝟑𝟖𝟒. 𝟐𝟔

STEINHART-HART EQUATION

𝑄 = ln 𝑆𝑞𝑏𝑗𝑠𝑓𝑒 × 𝑊𝑡𝑣𝑞𝑞𝑚𝑧 𝑊𝑝𝑣𝑢 − 𝑆𝑞𝑏𝑗𝑠𝑓𝑒

T: Temperature in degree Celsius A,B,C are Steinhart-Hart coefficients A: 1.129148 × 10-3 B: 2.34125 × 10-4 C: 8.76741 × 10-8

Software-based feedback loop using

the mean temperature

Used to sustain temperature at 28 ˚C

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RESULTS

HUMIDITY-

Graph of humidity against time to test the ability of the incubator in maintaining an optimal humidity range

2 wells with 10ml of water each were

used during the experiment

Humidity level of 85%-95% is ideal
At the optimum temperature of 28 ˚C,

humidity increases from 86.8% to 90.3%.

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DESIGN SPECIFICATIONS

Incubator designed Incubators on the market (average) Size

17.5 cm x 12 cm x 7 cm 0.654g 28 cm x 20 cm x 9 cm 1.5 kg

Cost

<$120 $15000-$20000 with microscope

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CONCLUSION

PHYSICAL FUCTIONAL TEMPERATURE LOW-COST COMPACT HUMIDITY

ENGINEERING GOALS

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FUTURE IMPROVEMENTS

HEATING ELEMENT VENTILATOR