Con ontrol ol of st stored d products cts pest sts s by - - PowerPoint PPT Presentation

Vlatka Rozman University of Josip Juraj Strossmayer in Osijek Faculty of Agriculture in Osijek, Croatia

Con

• ntrol
• l of st

stored d products cts pest sts s by natural al products

Nat Natural ral pro roduc ucts ts possess ss a spectrum rum of pro roper erti ties:

insecticidal activity repellence to pests antifeedancy insect growth regulation toxicity to mites and other pests antifungal, antiviral, antibacterial

Ad Advant antages ages of use

• They break down rapidly under the

influence of daylight and sunlight, moisture and rainfall.

Rapid degradation

• Typically kill insects quickly or prevent

their feeding immediately after application.

Quick action

• They are less stable and therefore have

smaller negative impact on beneficial and non-target organisms.

Low toxicity to warm-blooded

• rganisms
• They are not harmful to treated plants

(they are not or are slightly phytotoxic) when applied according to instructions.

Selectivity on treated plants

• Their rapid degradation, though favorable from

the standpoint of environmental protection and human health, is often requiring their frequent use.

Rapid degradation

• Although deemed to be considerably less toxic in

comparison to synthetic insecticides, certain botanical insecticides such as nicotine and rotenone are toxic to humans and fish.

Toxicity

• They are generally more expensive than synthetic

insecticides, many are not commercially available for a number of reasons including standardization

• f their production.

Price and availability

Disadv dvanta ntages es of use

The main n criteria eria fo for apply lying ing plant nts with insecti ticida idal l pro roper erti ties es

1.

• When choosing the appropriate plant species which is found to have insecticidal

substances, strict attention should be paid to preservation of ecosystems. 2.

• The plant should be widely spread in large numbers in nature.

3.

• Leaves, flowers and fruits, which means parts of plants that are regularly renewed

should be used mostly, and the root of the annuals if root is needed. 4.

• If using parts of perennial plants special care should be taken in order not to

exterminate the species (avoid using root of perennial plants). 5.

• Selected plants should occupy small area for cultivation, and be undemanding in

terms of tillage, water and nutrients. 6.

• The selected plant is preferred to be used in other areas such as health care,

pharmaceutical industry, food industry, etc. 7.

• Also, it is preferable that the plant does not have high economic value.

8.

• Active substance of the selected plant should be effective at lower concentrations.

More e significan icant t use of natural al produ ducts ts in practic tice will l be possib ible le if they are compatib atible le with h the following ing principles iples:

 safe  low toxicity  easy for application  minimal (or nil) problems with insecticide residues on foods  high efficacy at very low concentrations against stored grain insects,

adult pests and their progeny

 wide spectrum of efficacy against stored grain insect pests and field

pests

 low adverse effects on grain handling and quality properties  acceptable in terms of price

Ong ngoi

• ing researc

arch in n Cro roatia tia

Croatian Science Foundation (HRZZ) - call: IP-11-2013

• Approved funding a three-year research project

Project title: Development of new natural insecticide formulations based on inert dusts and botanicals to replace synthetic, conventional insecticides

• Project acronym: DIACROMIXPEST
• Project No. IP-11-2013-5570
• Period: from 1 July 2014 to 30 June 2017
• Project leader: Prof. Ph. D. Vlatka Rozman (FoA Osijek)

DIACROMIXPEST

DIA - DE and inert dusts CRO - Croatian

• rigin

MIX - mixture of substances PEST

• Prof. Vlatka Rozman
• Assist. Prof. Anita Liška

Assoc.Prof. Renata Baličević Pavo Lucić, MSc. Ing. Agr.

• Ph. D. Zlatko Korunić
• Prof. Josip Halamić

Ph.D. Ines Galović

Project idea:

Develop natural insecticide formulations Use substances

• f Croatian
• rigin

Develop new technology of mixing substances Apply in agriculture and related sectors Create a new Croatian product DIACROMIXPEST

DIACROMIXPEST

New natural insecticide formulation properties

Effective on harmful insects Based on Croatian inert dusts and botanicals Slow the emergence of resistance due to at least two mechanisms of action Safe and slightly toxic to warm- blooded organisms No negative effect on the treated products and the handling of working with the goods Reasonable price for users

New formulations should be 5 to 10 times less used in relation to DE when used alone. New formulations should provide complete protection with 90-99% insect mortality in stored products within 7 to 21 days. New formulations should become one of the bases of integrated protection measures.

DIACROMIXPEST

DIACROMIXPEST

Project activities :

Phase I

Field trials deposits of inert dusts and diatomite in Croatia Physical and chemical analysis of samples Determine the species of diatoms Select samples to test the effectiveness of the insects Select Croatian aromatic and food grade plants with insecticidal activity Investigate the methods and modes of mixing tested plant components and inert dusts

Phase II

Develop formulations of insecticides Select the best formulation for the safety of warm-blooded

• rganisms and efficiency to

insects Test the long-term activity of selected formulations on wheat and maize Analyze possible residues on treated wheat and maize Chemical analysis of selected formulations

Phase III

Test the stability of the new formulations under normal storage conditions and on the field Toxicological analysis of the new formulation of possible acute, oral, dermal and inhalatory toxicity Preliminary test formulation for pests in public health and communal hygiene, protection of plants in vegetation, animal health and food industry To give an answer whether Croatia has a new formulations of insecticides from its own resources

Zagreb and the Zagorje region: 6 locality 21 samples

13.5.2015. Službeni posjet HRZZ

Banovina region: 6 locality 23 samples

13.5.2015. Službeni posjet HRZZ

Požeška gora – Slavonia region: 2 locality 12 samples

13.5.2015. Službeni posjet HRZZ

13.5.2015. Službeni posjet HRZZ

13.5.2015. Službeni posjet HRZZ

Collecting and preliminarily test aromatic and food plants with a potential insecticidal activity:

• laurel (Laurus nobilis L.),
• lavender (Lavandula x intermedia),
• lemon balm (Melissa officinalis L.),
• mint (Mentha piperita L.),
• basil (Ocimum basilicum L.),
• marjoram (Origanum marjorana L.),
• oregano (Origanum vulgare L.),
• woodland sage (Salvia nemorosa L.),
• sage (Salvia officinalis L.),
• clary sage (Salvia sclarea L.),
• savory (Satureja hortenzis L.),
• wild thyme (Thymus serpyllum L.),
• garden thyme (Thymus vulgaris L.),
• sunflower (Helianthus annuus L.),
• rapeseed (Brassica napus L.).

13.5.2015. Službeni posjet HRZZ

Current ently ly status of research ch

Currently, research on the project is in the first “selective” phase. There are several potential Croatian samples of inert dusts and diatomite (Liška et al., 2015). Being tested formulations of powder and extracts from Croatian aromatic and food plant. Some of them are very potential, such as lavender (Lucić et al., 2015.).

DIACROMIXPEST

Ackn knowled wledgement gement

 Financial support was provided by the Croatian Science

Foundation (HRZZ):

 Scientific research project IP-11-2013-5570: “Development of

new natural insecticide formulations based on inert dusts and botanicals to replace synthetic, conventional insecticides“

 www.diacromixpest.eu

Thank nk you for your attentio tion!