Compound Light Microscope Light & glass lenses used to - - PowerPoint PPT Presentation

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Jan 09, 2023 322 likes •509 views

Compound Light Microscope Light & glass lenses used to magnify objects Eyepiece 10X Objective lenses: 4x,10x, 40x Up to 2000x Resolution Ability of microscope to show fine detail Wet Mounts Cover slips: protect lens from water,

SLIDE 1

Compound Light Microscope

Light & glass lenses used to magnify

bjects

SLIDE 2

Eyepiece 10X Objective lenses: 4x,10x, 40x Up to 2000x

SLIDE 3

Resolution

Ability of microscope to show fine detail

SLIDE 4

Wet Mounts

 Cover slips: protect lens from water,

keep specimen in place & protect them

 Field of View: how much of the

bject you can see

 stains

SLIDE 5

SLIDE 6

ScanningElectronMicroscope SEM

M______ lenses and electrons used to magnify

bjects

SLIDE 7

pollen

3D 100- 300,000X $30-100,000 Metal coating

SLIDE 8

SLIDE 9

Transmission electron microscope

SLIDE 10

Wasp virus 2D Up to 2,000,000X $100- 200,000 Metal coatings

SLIDE 11

Microtome (Uses diamond knife to

cut very thin slices of specimen)

SLIDE 12

Centrifuge to spin at

high speeds; breaks up cells; separates by density

SLIDE 13

Cell Fractionation

SLIDE 14

Cell Culture (to clone identical

cells)

HeLa cell line

http://www.youtube.com/watch?v=2mOroGqJ_Uk

SLIDE 15

Slide A

SLIDE 16

Slide B

SLIDE 17