Combination of biological control agents and fungicides to control - - PowerPoint PPT Presentation

Birgit Jensen

Dan F. Jensen, Thies M. Heick, Mukesh Dubey, Magnus Karlsson, Hans J.L. Jørgensen, David B. Collinge and Lise N. Jørgensen

Combination of biological control agents and fungicides to control diseases in wheat and reducing the risk of fungicide resistance

Septoria tritici blotch Fusarium head blight

What is the potential of using the fungal biocontrol agent Clonostachys rosea alone or in combination with Proline ?

• C. rosea, 1 x 107 conidia/m2

Proline 250 EC , 0.4 L/ha Applications: GS 37-39: (early) GS 61: (late)

20 40 60

• 14. June
• 25. June

a bc b bc bc bc c a bc bc bc b c b

Fusarium head blight Septoria leaf 2-3

Pervcent infection

Septoria flag leaf

Field trial 2019

Untreated Proline early Proline late Proline early+late

• C. rosea+Proline
• C. rosea early
• C. rosea early+late

hkg/ha

U n t r e a t e d P r

• l

i n e e a r l y P r

• l

i n e l a t e P r

• l

i n e e a r l y + l a t e C . r

• s

e a + P r

• l

i n e C . r

• s

e a e a r l y C . r

• s

e a e a r l y + l a t e 100 200 300 400 500

DON ppb

b b b b b a ab

grain yield DON content

Field trial 2019

Fungicide resistance in Zymoseptoria tritici associated with mutations in the CYP51 gene

Amino acid polymorphism in th CYP51 gene identified by PacBio sequencing analysis

• Isolate sensitivity tests (EC50 )
• Detection of CYP51 mutations

Supported by the Danish Environmental Protection Agency. Grant MST-667-00266

Poster No. 26 Birgit Jensen Hans J.L. Jørgensen David Collinge Lise N. Jørgensen Thies Marten Heick Dan Funck Jensen Magnus Karlsson Mukesh Dubey