Carinata Summit 2016 Update on Breeding Efforts in Carinata Growth - - PowerPoint PPT Presentation

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Carinata Summit 2016 Update on Breeding Efforts in Carinata Growth - - PowerPoint PPT Presentation

Jan 29, 2023 207 likes •341 views

Carinata Summit 2016 Update on Breeding Efforts in Carinata Growth of Agrisoma & Collaborator R&D teams Agrisoma

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Carinata Summit 2016 Update on Breeding Efforts in Carinata

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Growth of Agrisoma & Collaborator R&D teams

Agrisoma R&D Team Carinata “on the ground” R&D Teams Management

  • J. Klingenberg (VP Product

Development)

  • M. Lindenbaum (VP

Technology)

  • D. Males (Director Breeding &

Agronomy)

  • M. Lortie (Director Regulatory)

Saskatoon labs

  • R. Bennett (Breeder)
  • S. Rooke (Asst. breeder)
  • M. Moore (DH lead)
  • K. Czechowicz (DH group)
  • V. Catinot (DH group)
  • T. Fowler (DH group)
  • K. Livingstone (DH group)
  • E. Hill (Crossing specialist)
  • P. Fu (Genetic diversity)
  • E. Risseeuw (Molecular)
  • L. Chen (Molecular)
  • T. Hoffman (Analytics)

Greenhouse

  • M. Troesch (GH Lead)
  • S. Klashinsky (GH)

Field team

  • C. Bliss (Agronomist)
  • M. Henke (Agronomist)
  • K. Wilson (Agronomist)
  • R. Attri (Nursery Manager)

INIA Uruguay Lizama Farms Chile University of Florida Auburn University South Dakota State University North Dakota State University Montana State University United States Department of Agriculture (various extensions) Agriculture and Agri-Food Canada National Research Council of Canada Agri-ARM Groups (Western Canada) Westwind Ag Research Farming Smarter Mustard 21 + Other groups involved in testing of oil, meal, processing, etc.

R&D efforts enhanced through collaborative effort and development of local expertise in each geography

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Progress in experimental line production

1000 2000 3000 4000 5000 6000 7000 8000 9000

  • No. of DH lines field evaluated

Project Total no. plantlets analyzed

  • No. doubled

(2N) Ratio fertile Cross set #1 10,341 5,443 52.6% Cross set #2 4,138 2,227 53.8% Cross set #3 10,157 3,205 31.6% Totals for 2015 24,636 10,875 44.1%

Target: Consistently produce 8-10K DH lines per year Simultaneous, efficiency increased by removing lines at early stage using: i) Molecular markers (beginning to use first set of markers) ii) Flow cytometer (removes haploid plants at plantlet stage) DH team effort: Produce large number lines on front end to reach goals, increase chance of success Molecular team effort: Marker discovery phase, implement markers in high throughput system

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Progress in Agronomic Potential of Experimental lines

50 100 150 200 250 300 350 400 450 500 550 600

5 or less 6 7 8 9

2014 Summer 2015 Summer 2015-16 Winter 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300

5 or less 6 7 8 9

2014 Summer 2015 Summer 2015-16 Winter

  • No. of lines evaluated

Agronomic Impression Rating; Higher is better, Checks = ~7 DH lines Inbred selections

Cycle Percent of total rated 7 or better 2014 Summer 25% 2015 Summer 40% 2015-16 Winter 87% Cycle Percent of total rated 7 or better 2014 Summer 7% 2015 Summer 31% 2015-16 Winter 83%

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Progress in Test Hybrid production

  • No. of A-line

selections Estimated genetic distance from A120 13 0.400 – 0.450 18 0.350 – 0.400 2 0.300 – 0.350 0.250 – 0.300 0.200 – 0.250 2 0.150 – 0.200 1 <0.150 12 Data not available Average all selections 0.392

  • 269 unlinked SNP loci used in analysis, representing all chromosomes
  • Higher fraction indicates greater dissimilarity

Current:

  • Generating seed for two R-lines and twenty A-lines for test

hybrid production Summer 2016:

  • Initiate production of test hybrid seed
  • Target: 20 A-lines x 2 R-lines = Seed for 40 test hybrids

produced Winter 2016-17:

  • Target: 20 additional A-lines x 2 R-lines = Seed for 40

additional test hybrids produced Summer 2017:

  • First set of 80 test hybrids in yield trials
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Progress in marker discovery: NAM project

Phenotyping beginning soon: 2016 N. Tier “test run”

  • 51 Founder Lines

2016-17 S. Tier

  • Inbred population

phenotyping (2,500 lines Commercially relevant traits: Includes traits such as

  • Early vigour
  • Flowering, maturity
  • Branching
  • Thousand seed weight
  • Seed quality parameters
  • Etc. etc.
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“Southern Tier” product development: SE United States and Uruguay

Three high priorities, besides yield per se: 1. Days to maturity 2. Frost tolerance 3. Very good shatter tolerance Uruguay Testing 2015 - La Estanzuela, Uruguay

Entry Days to maturity (vs. A110) Shatter loss estimates (kg ha-1) (% of yield) Yield estimate (kg ha-1) (% vs. A110) 159-4A1D 175 (Even) 69 (1%) 6155 (126%) 044-312D 170 (-5 days) 45 (1%) 5680 (116%) 044-3B1 175 (Even) 231 (4%) 5371 (110%) 159-1N1A 175 (Even) 67 (1%) 5309 (109%) 02.023 169 (-6 days) 48 (1%) 5039 (103%) 945-Z.063 176 (+1 day) 174 (4%) 4900 (100%) A110 175 168 (3%) 4891 (100%) 044-3111 177 (+2 days) 355 (8%) 4555 (93%) 40.168 176 (+1 day) 272 (7%) 3806 (78%)

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“Southern Tier” product development: Early maturity nursery

Cross Parental DTM vs. A110 in N. Tier

  • No. lines

#137

  • 1 x -5

28 #145

  • 7 x -3

237 #157

  • 5 x -1

522

  • Earliest flowering 10% of lines from each

population selected and self-pollinated

  • Goal for 2015-16: Identify line with ≥ 7 days

earlier maturity than A120

  • Future crosses using parents evaluated in short

day, winter environment (vs. long day, summer in current nursery) Early maturity nursery, Feb 22, 2016

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“Southern Tier” product development: Freeze tolerance

Name Rating - Site 1 Rating - Site 2 868-22B 40 20 25.102 50 30 439-A1 50 30 859-1 50 20 349-M22B 50 30 207-112 50 40 044-3B1 60 30 949-BI 60 20 044-3111 60 40 044-312D 70 40 159-11A2 70 50 AAC A120 70 50 AAC A110 70 40 044-3A21 80 40 400-A13C 80 50 40.063 80 40 19.308 80 60 137-5.106 90 40

More frost damage Less affected

  • Rating indicates estimate of % plot

damaged, three weeks after hard frost

  • Green indicates entries that were better

than checks in FL 2013-14 trials Frost tolerance ratings: Quincy 2014-15

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“Southern Tier” product development: Line ‘312D’

Isolation field “M-04”, Chile 2015-16 Rogueing Crew, Isolation field “HP-15” Seed harvested from Isolation field “M-01”

  • High yield potential variety in Florida and Uruguay

testing

  • Six isolation fields of top selections in Chile, rogueing

and colour sorting for more uniform type

  • Corresponding selections in 2015-16 FL yield trials
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“Southern Tier” product development: Line ‘312D’

Isolation tent “HP-06”, Chile 2015-16 Trial % seed yield of current check variety 2014-15 Quincy, FL (No Cadre residue) 123% 2014-15 Quincy, FL (Cadre residue) 109% 2015 Uruguay yield trial 116% 2015 Uruguay strip trials (avg. 5 sites) 113%

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Future direction: Breeding efforts

 Expand germplasm screening in Florida winter nursery: 2015-16 = 3 crosses, 800 lines screened for early maturity; 2016-17 = Two frost tolerant cross populations generated thus far  Expand validation of germplasm in Uruguay yield testing: 2015 = 10 entries x 1 site; looking at an additional site in 2016?  Numbers of DH lines produced for field testing will continue to increase  Identification and use of molecular markers continuing to become more important part

  • f program, efficient selection of lines going into field testing

 Collaboration among Agrisoma and many partners in a number of geographies makes the breeding program stronger; “eyes” on the ground and local expertise