Breeding for nutritional traits in tomato using naturally occurring - - PowerPoint PPT Presentation

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Breeding for nutritional traits in tomato using naturally occurring - - PowerPoint PPT Presentation

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Breeding for nutritional traits in tomato using naturally occurring variation in the fruit specific beta-cyclase promoter Caleb Orchard, David Francis Ph.D. Kopec et al. 2012 Carotenoids in Tomato B Ronen et al. 2010 Does variation in the

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Breeding for nutritional traits in tomato using naturally

  • ccurring variation in the fruit specific beta-cyclase

promoter

Caleb Orchard, David Francis Ph.D.

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Kopec et al. 2012

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Carotenoids in Tomato

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B

Ronen et al. 2010

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Does variation in the promoter of the B gene lead to different levels of beta-carotene in tomato fruit?

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Sources of variation

Juane Flammee – S. lycopersicum LA716 – S. pennellii 97L97 – S. cheesmanii

John R. Stommel. HortScience April 2001 vol. 36 no. 2 387-388

Does variation in the promoter of the B gene lead to different levels of beta-carotene in tomato fruit?

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Promoter Variation

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Promoter Variation

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Promoter Variation

2010 Dalal et al., BMC Plant Biology 2010, 10:61

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Population Development

Recurrent Parent

  • OH8245

Donor Parents

  • LA3501 (M-82)
  • LA3502 (M-82)
  • Juane Flammee

Recurrent Donor

  • Y. Eshed and D. Zamir. Genetics. 1995 November; 141(3): 1147–1162.

BC1 BC2 BC3

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SolCA lCAP SNP A Array rray

Sim S-C, Van Deynze A, Stoffel K, Douches DS, et al. (2012) High-Density SNP Genotyping of Tomato (Solanum lycopersicum L.) Reveals Patterns

  • f Genetic Variation Due to Breeding. PLoS ONE 7(9): e45520. doi:10.1371/journal.pone.0045520

http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045520

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Marker Selection

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Marker Selection

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Marker Selection

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Marker Selection

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MAS

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Distribution of Progeny vs. % Recurrent Genome in BC1

% Recurrent Parent Genome # of Progeny

Average RP Genome 75.50% Population 1 74.45% Population 2 76.00% Population 3 Selections: 84.9 – 89.6% (a generation ahead)

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Future work

/ BC2

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Conclusions

Multiple versions of fruit-specific beta-cyclase promoter Marker resources allow us to distinguish genetic backgrounds (M82 vs. OH8245, JF vs. OH8245) BGS facilitates selection of individuals one generation “ahead” each cycle

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Plant Genetic Resources for Human Health and Nutrition Research

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Acknowledgements

  • Ohio State

– David Francis – Steven Schwartz – Sung-Chur Sim – Heather Merk – Nancy Huarachi – Troy Aldrich – Cassi Sewell – Rachel Kopec – Jessica Cooperstone

  • UC-Davis

– Allen Van Deynze – Kevin Stoffel – Alex Kozic

  • Michigan State

– David Douches – Robin Buell – John Hamilton – Dan Zarka – Kelly Zarka

  • Cornell

– Walter De Jong – Lucas Mueller

  • USDA-Beltsville

– John Stommel

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Thank you