ARS Provides Opportunities to Integrate Research and Teaching with - - PowerPoint PPT Presentation

ARS Provides Opportunities to Integrate Research and Teaching with Industry – 2016

An accidental deletion of vitamin D spun into career-training for students

Tom Crenshaw Department of Animal Sciences University of Wisconsin-Madison

Swine Research & Teaching Center

One Example: Vitamin D-Induced Kyphosis

CALS Board of Visitors – April 8, 2016

My Focus for Today:

Research - an opportunity to train students Critical role for the ARS Research Stations

Swine Research & Teaching Center

Kyphosis – idiopathic disease induced by

vitamin D, Ca & P deficiencies

Rortvedt et al., 2012.

The hump-back pig challenge First Incidence at SRTC occurred in an AS 415 class project

Kyphosis

Mycotoxins Disease Genetics Diet Yes Yes Yes Yes No No No No Time Course Yes No

Vitamin D-Induced Kyphosis

Laura (Rortvedt) Amundson

Initial project supported by Cargill-Benevenga Undergraduate Research Stipend

ASAS Young Scholar - 2016

Hypovitaminous D Kyphosis Pig Model

• Spontaneous outbreak of kyphosis in UW

herd in 2008 attributed to an accidental deletion of vitamin D from premix

• Reproduced kyphosis under controlled

conditions (Rortvedt and Crenshaw, 2009)

Validation of the NCCC-42 Vitamin-Trace Mineral Premix in Grower Pigs

T.D. Crenshaw*1, M.D. Lindemann2, H.H. Stein3, and NCCC-42 Swine Nutrition Committee1 University of Wisconsin1, University of Kentucky2, and South Dakota State University3

2004 Adopted the NCCC-42 Vitamin-Trace Mineral Premix formulation for the UW SRTC herd diets

The rise and fall of clinical cases of vitamin D deficiency in commercial swine operations

Darin Madson Iowa State University

Veterinary Diagnostic Laboratory

Iowa State University

Dramatic increase attributed to cases of hypovitaminosis D

Femur

Lesions of Uncoupled Endochondral Ossification?

Hypovitaminosis D Induced Kyphosis

Abnormalities detected in multiple locations

Vertebrae

• D

+D

Which cellular signals are altered by hypovitaminosis D to induce gross bone abnormalities?

Invading capillary Osteoblast Resting zone Proliferative zone Hypertrophic zone Osteoclast

Type II Collagen Type X Collagen MMP13 VEGF MMP9 MMP13 VEGF Type I Collagen

Hypovitaminous D Kyphosis Pig A model to study bone lesions Contributions to date:

• 25 students trained
• Engaged in interactions with Swine Industry Managers, Nutritionists,

Veterinarians; Bone and Nutrition researchers; Orthopedic and Biomedical researchers

• Output – 16 research presentations at scientific meetings; 8 invited

presentations (3 international); 1 8-hr symposia; 3 conference proceeding published; 3 manuscripts published

• All possible because we could reproduce kyphosis under controlled

conditions

Swine Research & Teaching Center

SRTC Staff Jamie Reichert Jeff Booth Sam Trace Keri Graff Deb Schneider

Students Involved with vitamin D research Laura Amundson Zainab Hassen Lauryn Vanderwerf, Olivia Colonero Pete Strassburg, Faiza Khan Connie Chuang, Lauren Zappitelli Michelle McMahon, Leigh Danner Ben Giese, Joe Jacobson, Paul Merkatoris Kelli Retallick, Becky Keel Alex Agar, Yvonne Schulke Jennifer Babcock, Anna Masad Katie Wihelms, Rachel Hartlaub, Charlie Wasserburger Lynzie Miller, Sarah Witt Mariola Grez

Acknowledgements

Laura Amundson - Ph.D. Committee Tom Crenshaw, Laura Hernandez, Margaret Dame, Dan Schaefer, Brian Kirkpatrick

Swine Research & Teaching Center

Tom Crenshaw - Department of Animal Sciences

SRTC

Swine Research & Teaching Center

Researchers supported Crenshaw Halanski Sexing Tech Reed Lamers Zinpro Kreuger Tanumihardjo CB Farms/Trimuph Dhanu Penniston Murphy-Brown Parrish Abrams NCCC42 Swine Nutr. Sindelar Ploeg Cardiovascular Res Found. Steele Bromage UW Swine Ext Team ISU Swine Path. Merck Agriculture Agri-Food Canada

• Univ. Minnesota

WI Corn Growers Granco