ACB Yogurt Dermal Respiratory Factor PF 20224PF ACB Yogurt Dermal [PDF]

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ACB Yogurt Dermal Respiratory Factor PF

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20224PF ACB Yogurt Dermal Respiratory Factor PF

Cellular Respiration + Collagen Production + Probiotic + Soothing + ATP Synthesis

Product Code: 20224PF INCI Name: Lactobacillus Ferment Lysate Filtrate INCI Status: Conforms Suggested Use Levels: 2.0 – 5.0% Suggested Applications: Cellular Respiration, Collagen Production, Probiotic, Soothing, ATP Synthesis

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ACB Yogurt Dermal Respiratory Factor PF is non-animal derived + non-dairy
ACB Yogurt Dermal Respiratory Factor PF is produced by prompting live Lactobacillus bulgaricus

cells to secrete stress response elements by striking the Lactobacillus cells with specific wavelengths of UV radiation.

The secretion is isolated and extracted from the live bacteria cell to deliver various anti-aging

benefits and anti-irritant properties.

ACB Yogurt DRF PF is capable of increasing oxygen uptake while stimulating collagen synthesis and

enhancing cellular functions.

These elements isolated from bacteria, also have anti-irritant properties and are capable of

modulating wound healing.

20224PF ACB Yogurt Dermal Respiratory Factor PF

Product Overview

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Increases oxygen uptake/cellular respiration
Antioxidant
Enhances ATP Synthesis/cellular energy
Enhances collagen production
Increases fibroblast proliferation
Reduces Redness - Soothing

20224PF ACB Yogurt Dermal Respiratory Factor PF

Cosmetic Benefits

Ideal for use in baby care, after sun care, sensitive skin, scalpcare applications and to provide potent anti-aging benefits

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20224PF ACB Yogurt Dermal Respiratory Factor PF

Oxygen Uptake Assay

Protocol

Increased oxygen uptake indicates

cellular respiration

Cultured human fibroblasts were used
According to the results, 5% ACB

Yogurt Dermal Respiratory Factor PF can substantially increase oxygen uptake

Figure 1: Oxygen Uptake of ACB Yogurt Dermal Respiratory FactorPF compared toLYCD.

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20224PF ACB Yogurt Dermal Respiratory Factor PF

Collagen Production Assay

Protocol

Collagen I is a major component of

the dermis & provides structure & elasticity to the skin

Three dose levels were compared to

the control – 1%, 2% & 4%

Results were determined by the

ELISA Assay

According to the results, 4% ACB

Yogurt Dermal Respiratory Factor PF was most effective at increasing Collagen I synthesis

Figure 2: Increase in collagen production following application of ACB Yogurt Dermal Respiratory Factor PF .

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20224PF ACB Yogurt Dermal Respiratory Factor PF

ATP Synthesis Assay

Protocol

An increase in ATP synthesis

demonstrates an increase in cellular metbolism

2.0% ACB Yogurt Dermal Respiratory

Factor PF was tested

After 24 hours there was a 42% increase

in ATP levels

ACB Yogurt Dermal Respiratory

Factor PF showed a significant increase in ATP levels compared to the control

Figure 3: Increase in ATP Synthesis following application of ACB YogurtDermal Respiratory Factor PF

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20224PF ACB Yogurt Dermal Respiratory Factor PF

ORAC Assay

Protocol

Trolox is used as the positive control
Solutions were prepared at two

concentrations for a reference

Fluorescent measurements were

taken every 2 minutes for 2 hours

ACB Yogurt Dermal Respiratory Factor PF

at 2.0% & 4.0%.

Figure 4: Results of antioxidant capacity of testmaterials.

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20224PF ACB Yogurt Dermal Respiratory Factor PF

IL-6 ELISA Assay

Protocol

Human dermal fibroblasts seeded into 12-well

tissue culture plates

ACB Yogurt Dermal Respiratory Factor PF

added to complete DMEM containing 1µg/mL LPS and incubated with fibroblasts for 24 hours

Concentrations: 1%, 0.1%, 0.01%
Decrease in IL-6 production indicates a

reduced inflammatory environment which could decrease the signs of aging and reduce the formation of fine lines and wrinkles

Figure 5: ACB Yogurt Dermal Respiratory Factor PF-treated fibroblasts IL-6 concentrations

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20224PF ACB Yogurt Dermal Respiratory Factor PF

Anti-Irritation Assay

Protocol

10 (m/f) subjects
Tested materials: base lotion + 5.0% ACB Yogurt

Dermal Respiratory Factor PF vs. Base Lotion

Treatments were applied to irritated patches
Irritation was induced via tape stripping
Subjects then applied equal amounts of both

lotions immediately after irritation

Evaluate and rate them on a rubric of 1 to 10 after

15 minutes

42.0% reduction in irritation was perceived 15

minutes after application of the base cream with 5.0% ACB Yogurt Dermal Respiratory Factor PF

Figure 6: Average anti-irritancy ratings for ACB Yogurt Dermal Respiratory FactorPF compared to theplacebo.

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20224PF ACB Yogurt Dermal Respiratory Factor PF

Anti-Irritation Assay

Methods

Pictures were taken right after

irritation & 15 minutes after application of 5% ACB Yogurt DRF PF

The images show a significant

reduction in redness/irritation after using ACB Yogurt DRF PF

Figure 7: Before + After Photos Figure 8: Before + After Photos

Control ACB Yogurt DRF Control ACB Yogurt DRF

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20224PF ACB Yogurt Dermal Respiratory Factor PF

Cellular Respiration + Collagen Production + Probiotic + Soothing + ATP Synthesis

Product Code: 20224PF INCI Name: Lactobacillus Ferment Lysate Filtrate INCI Status: Conforms Suggested Use Levels: 2.0 – 5.0% Suggested Applications: Cellular Respiration, Collagen Production, Probiotic, Soothing, ATP Synthesis

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