AC Dermal Respiratory Factor Advanced PF Increases Cell Respiration - - PowerPoint PPT Presentation

AC Dermal Respiratory Factor Advanced PF

Anti-Aging Technology Using Saccharomyces cerevisiae

Increases Cell Respiration + Collagen & Elastin Synthesis + Enhances Cell Metabolism

AC Dermal Respiratory Factor Advanced PF

Technical Information:

Product Code: 20219PF INCI Name: Water + Saccharomyces Lysate Extract INCI Status: Conforms Suggested Use Levels: 0.5 – 1.0% Suggested Applications: Increases Cellular Respiration + Enhances Cellular Metabolism + Collagen/ Elastin Synthesis + Anti-Aging Solubility: Water Soluble

AC DERMAL RESPIRATORY FACTOR ADVANCED PF

Saccharomyces cerevisiae

• Saccharomyces cerevisiae live yeast cells are struck with specific

wave lengths of UV radiation  Prompts live yeast cells to secrete active compound  Biofermenation and various filtration techniques are used to then isolate and extract the live yeast secretion

• Non-Animal Derived + Non-Dairy
• Secretion produced from the most well-known yeast

 Baker’s Yeast/ Brewer’s Yeast

AC DERMAL RESPIRATORY FACTOR ADVANCED PF

Product Benefits:

 Increases cellular respiration  Increases cellular proliferation  Enhances cellular metabolism  Promotes collagen + elastin synthesis  Extraordinary skin soothing properties Ideal for use in baby care, after sun care, sensitive skin, scalp care applications and to provide potent anti-aging benefits

Live Yeast Cell Derivatives (LYCD)

LYCD

• Commercial use began in the American Home Product’s market

 LYCD was the main active in an anti-haemorrhoid cream – Prep H

• Adventurous fashion models can be thanked for the crossover of LYCD

into cosmetic applications for soothing

• Sleep deprivation caused bags under the eyes
• Models used this LYCD containing cream to reduce the

appearance of bags and swelling

In North America + South America + Europe LYCD is primarily a cosmetic potentiater:  Stimulates Oxygen Consumption  Combats Irritation

Reduces Irritation: Mechanism of Action

Live Yeast Cell Derivatives

• LYCD’s role in irritation reduction became clearer with

a more focused study of Heat Shock Proteins (HSP)

• When cells are subjected to stress, proteins are

produced to:  Duplicate the functions of disabled cell  Protect the cell against additional stress

• HSPs are a component of LYCD – these specific

Heat Shock Proteins are similar to human cells & can be applied to help reduce or reverse the actual stress in human cells

Potentiation of Activity

Live Yeast Cell Derivatives

• LYCD is a global metabolic stimulant
• Capable of potentiating the activity of other materials in a cosmetic/

personal care formulation

• LYCD utility is evidenced in Avon’s patents US 5,676,956 and US 5,643,587

where it’s used to increase the activity of Vitamin C derivatives

Yeast Species

Saccharomyces cerevisiae

• Fermentation abilities
• Very similar to human cells in composition and structure
• True eukaryote
• Only 17 chromosomes (humans have 23) but

structurally similar

• Two genders, reproduces with cells (similar to

human cell fertilization)

• First organism to have a completely mapped genome
• Egyptians are thought to have discovered the beneficial

properties of yeast:

• Over 6,000 years ago
• Leavened breads, wine and beer

Yeast + Fermentation

Discovering Fermentation

• Said that yeast cells found their way to an open barrel of

crushed grapes and the barrel contents turned into wine!

 Residual yeast sediment was then added to

subsequent grape barrels

 Wine production process born!

• In the 1850’s, Louis Pasteur discovered that

fermentation was simply the metabolic by-product

• f yeast
• In 1896, German chemist Eduard Buchner

discovered that fermentation enzymes in yeast remained active even after cell extraction – this paved the way for the delineation of biochemical process of fermentation

Yeast + Biofermentation

• Dr. George Sperti
• 1930’s – Performed research using UV radiation and yeast cells
• Live yeast cells were injured with UV radiation and secretions from the cells

were collected

• His findings and methods of LYCD production were immortalized

in three patents!

• Discoveries after secretion extraction was added

to normal cells:

Significant Increase in Cellular Respiration + Increase in Cellular Replication + Enhanced Glycolysis + Enhanced Cellular Metabolism

 Isolated LYCD were shown to stimulate cells from other eukaryotes 

In-vivo Sensorial Soothing Assay

Protocol

• Evaluate the ability of AC Dermal

Respiratory Factor Advanced PF to reduce the discomfort produced by

• verexposure to UV radiation
• 20 panelist (m/f) evaluated two

lotions containing either AC DRF Advanced PF (2.8% w/w) or Benzocaine (0.5% w/w)

• Panelists applied lotions

immediately after sun exposure

• Recorded initial soothing

perception and perception after 2 hours (1 to 5 with 5 being complete reduction in pain) Topical application of AC Dermal Respiratory Factor Advanced PF is capable of producing consumer perceivable reduction in both erythema and pain caused due to overexposure to UV light.

AC Dermal Respiratory Factor Advanced PF Efficacy Test Results

0.1 0.2 0.15 1.45 1.15 1.3 3.6 3.95 0.5 1 1.5 2 2.5 3 3.5 4 4.5 t = 0 Hr t = 2 Hr t = 0 Hr t = 2 Hr t = 0 Hr t = 2 Hr t = 0 t = 2 Hr Benzocain AC DRF Advanced PF Benzocaine AC DRF Advanced PF Reduction of Erythema Reduction of Sunburn Pain Perceived Reduction

AC Dermal Respiratory Factor Advanced PF Efficacy Test Results

30 60 90 120 150 180 50mg/mL IGF-1 .01% AC DRF Advanced .1% AC DRF Advanced 1.0% AC DRF Advanced

Percent (%) Increase

Protocol

• Collagen is a major component of

the dermis & provides structure & elasticity to the skin

• Three dose levels were compared

to the control – .01%, .1% & 1%

• Results were determined by the

ELISA Assay

• According to the results, all

concentrations of AC Dermal Respiratory Factor Advanced PF was effective at increasing collagen synthesis

In-vitro Collagen Production Assay

Figure 2. Increase in collagen production following application of AC Dermal Respiratory Factor Advanced PF .

AC Dermal Respiratory Factor Advanced PF Efficacy Test Results

Procollagen Assay

Protocol

• An ELISA assay was used to

assess AC DRF Advanced impact

• n collagen synthesis
• The results indicate that AC DRF

Advanced PF is capable

• f

increasing the expression of type 1-C Peptide in a fibroblast cell culture model

• Useful in cosmetic formulations

intended to increase collagen synthesis

Figure 3. Comparison of the effects on procollagen levels following treatment.

500 1000 1500 2000 2500 3000 3500 1% Magnesium Ascorbyl Phosphate 0.5% Magnesium Ascorbyl Phosphate 1% AC DRF Advanced PF 0.5% AC DRF Advanced PF 4mM Sodium Butyrate Untreated

Type 1 C-Peptide (ng/ml)

AC Dermal Respiratory Factor Advanced PF Efficacy Test Results

ORAC Assay

97 13 116 46 15 20 40 60 80 100 120 140 100µM Trolox 12.5µM Trolox 2% 20219 AC DRF Advanced 1% 20219 AC DRF Advanced 0.5% 20219 AC DRF Advanced

Antioxidant Capacity (µMTE)

Protocol

• Trolox is used as the positive control
• Solutions were prepared at two

concentrations for a reference

• Fluorescent measurements were

taken every 2 minutes for 2 hours

• AC Dermal Respiratory Factor

Advanced PF showed antioxidant activity at all concentrations tested.

Figure 4. Results of antioxidant capacity of test materials.

AC Dermal Respiratory Factor Advanced PF Efficacy Test Results

Pigmentation Assay

Protocol

• Equipment: DermaLab Skin Combo
• 10 volunteers M/F between the ages of

23 and 45

• Measure the erythema levels of the

subject’s volar forearms

• Baseline pigmentation readings were

taken on day one of the study

• AC Dermal Respiratory Factor Advanced

PF is capable of decreasing erythema when compared to both the untreated control as well as the base lotion

Figure 5. Pigmentation Results for Individual Test Sites.

10.00 10.50 11.00 11.50 12.00 12.50 13.00 13.50 T = 0 T = 1 Week T = 2 Weeks T = 3 Weeks T = 4 Weeks

Erythema

Average Results for Individual Test Sites

Experimental (2.0% AC DRF Advanced PF + Base Lotion) Base Lotion Untreated

AC Dermal Respiratory Factor Advanced PF Efficacy Test Results

Pigmentation Assay

Protocol

• Equipment: DermaLab Skin Combo
• 10 volunteers M/F between the ages
• f 23 and 45
• Measure the erythema levels of the

subject’s volar forearms

• Baseline pigmentation readings were

taken on day one of the study

• AC Dermal Respiratory Factor

Advance PF showed reductions in erythema at a 2.0% concentration

Figure 6. Pigmentation Results Compared in Percent Difference between Test Sites.

• 20.00%
• 15.00%
• 10.00%
• 5.00%

0.00% 5.00% 10.00%

T = 0 T = 1 Week T = 2 Weeks T = 3 Weeks T = 4 Weeks

Percent (%) Difference

Erythema

Comparative Data Between Two Sites

Base Lotion vs. Untreated Experimental (2.0% AC DRF Advanced PF + Base Lotion) vs. Untreated Experimental (2.0% AC DRF Advanced PF + Base Lotion) vs. Base Lotion

AC Dermal Respiratory Factor Advanced PF Efficacy Test Results

High Resolution Ultrasound Skin Imaging Assay

Protocol

• Equipment: DermaLab Skin Combo
• 10 volunteers M/F between the ages
• f 23 and 45
• Determine the skin density of the

subject’s volar forearms

• Apply 2 mg of each test material on

their volar forearms

• Concentration: 2%
• AC Dermal Respiratory Factor

Advanced PF is capable of improving skin density when compared to both the untreated control as well as the base lotion

Figure 7. Ultrasound Results Comparing Test Sites to Untreated Site.

• 10.00%
• 5.00%

0.00% 5.00% 10.00% 15.00% 20.00% 25.00% T = 0 T = 1 Week T = 2 WeeksT = 3 WeeksT = 4 Weeks Percent (%) Difference

Collagen Ultrasound

Comparison of Experimental + Control to Untreated Site

Base Lotion vs. Untreated Experimental (2.0% AC DRF Advanced PF + Base Lotion) vs. Untreated

AC Dermal Respiratory Factor Advanced PF Efficacy Test Results

Hyaluronic Synthesis Assay

Protocol

• In-vitro Hyaluronic Acid Synthesis was

performed using human keratinocytes

• Cultured in KSFM medium and incubated for a

period of 24 hours at a temperature of 37C with the concentration of carbon dioxide in the incubator being limited to 5%

• Concentrations: 0.5%, 1.0%
• Incubated under the same conditions as before

for a period of 72 hours

• AC Dermal Respiratory Factor Advanced PF is

capable of increasing the percent concentration

• f hyaluronic acid by approximately 42% in

comparison to the control

Figure 8. Hyaluronic Acid Production comparing AC Dermal Respiratory Factor Advanced PF and Control.

20 40 60 80 100 120 140 160 Control 0.5% AC Dermal Respiratory Factor Advanced PF 1.0% AC Dermal Respiratory Factor Advanced PF

Percent (%) Increase

AC Dermal Respiratory Factor Advanced PF Efficacy Test Results

Cellular Viability Assay Analysis

Protocol

• Human dermal fibroblasts were seeded into

96-well tissue culture plates

• Concentrations: 0.1%, 0.01%
• AC Dermal Respiratory Factor Advanced PF,

nor the preservatives exhibited any inhibition of cell viability

• It can therefore be concluded that at normal

use concentrations AC Dermal Respiratory Factor Advanced PF is not cytotoxic

• 15%
• 10%
• 5%

0% 5% 10% 15% 20000 40000 60000 80000 100000 120000 Complete Media 0.1% AC Dermal Respiratory Factor Advanced PF 0.01% AC Dermal Respiratory Factor Advanced PF MFU MFU % Change

Figure 9. Cellular Metabolism of AC Dermal Respiratory Factor Advanced PF- treated fibroblasts expressed in terms of percent of control.

AC Dermal Respiratory Factor Advanced PF Efficacy Test Results

Moisturization Assay

Protocol

• Equipment: DermaLab Skin Combo
• 10 volunteers M/F between the

ages of 23 and 45

• Apply on volar forearms
• Moisture levels were improved by

44.60% after 24 hours and by 95.0% after 4 weeks when compared to the untreated control

• When compared to the base

cream, AC DRF Advanced PF improved moisturization by 33.74% after 4 weeks

0.00 50.00 100.00 150.00 200.00 250.00 T = 0 T = 24 Hours T = 1 Week T = 2 Weeks T = 3 Weeks T = 4 Weeks T = -24 Hours T = -1 Week Moisturization (μSiemens)

Average Moisture Readings

Experimental (2.0% AC DRF Advanced PF + Base Lotion) Base Lotion Untreated

Figure 10. Average increase in moisturization per test site.

AC Dermal Respiratory Factor Advanced PF Efficacy Test Results

Moisturization Assay

0.00 10.00 20.00 30.00 40.00 50.00 60.00 70.00 80.00 90.00 100.00 T = 0 T = 24 Hours T = 1 Week T = 2 Weeks T = 3 Weeks T = 4 Weeks T = -24 Hours T = -1 Week Percent (%) Difference Between Test Sites

Percent (%) Difference

Base Lotion vs. Untreated Experimental (2.0% AC DRF Advanced PF + Base Lotion) vs. Untreated Experimental (2.0% AC DRF Advanced PF + Base Lotion) vs. Base Lotion

Figure 11. Percent difference in moisturization between two test sites

• ver four weeks.

Protocol

• Equipment: DermaLab Skin Combo
• 10 volunteers M/F between the ages
• f 23 and 45
• Apply on volar forearms
• 2.0% AC DRF Advanced PF + Base

Lotion moisturized the skin 19.76% better after 24 hours and was still 9.01% more effective in moisturizing the skin when reading were taken

• ne week after the applications of

both test materials ceased

AC Dermal Respiratory Factor Advanced PF Efficacy Test Results

Moisturization Assay

Figure 12. Regression in skin moisturization after application of experimental material ceased.

Protocol

• Equipment: DermaLab Skin Combo
• 10 volunteers M/F between the

ages of 23 and 45

• Apply on volar forearms
• AC Dermal Respiratory Factor

Advanced PF is not only capable

• f providing functional benefits

but it is also capable of providing moisturizing and skin hydrating benefits when added to cosmetic applications

0.00 10.00 20.00 30.00 40.00 50.00 60.00 70.00 80.00 90.00 100.00 T = 0 T = 24 Hours T = 1 Week T = 2 Weeks T = 3 Weeks T = 4 Weeks T = -24 Hours T = -1 Week Percent (%) Difference

Moisture Regression

Experimental Treatment vs. Untreated

AC Dermal Respiratory Factor Advanced PF Efficacy Test Results

TEWL Assay

Protocol

• Equipment: DermaLab Skin Combo
• 10 volunteers M/F between the

ages of 23 and 45

• Measure TEWL on the subject’s

volar forearms; apply 2 mg of each test material

• AC Dermal Respiratory Factor

Advanced PF showed very effective moisture retention capabilities at a 2.0% concentration

0.00 0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 T = 0 T = 1 Week T = 2 Weeks T = 3 Weeks T = 4 Weeks Open Chamber Water Levels

Average Moisture Loss Readings

Experimental (2.0% AC DRF Advanced PF + Base Lotion) Base Lotion Untreated

Figure 13. TEWL measurements taken at individual test sites

AC Dermal Respiratory Factor Advanced PF Efficacy Test Results

TEWL Assay

Protocol

• Equipment: DermaLab Skin Combo
• 10 volunteers M/F between the

ages of 23 and 45

• Measure TEWL on the subject’s

volar forearms; apply 2 mg of each test material

• When compared to the base cream,

AC Dermal Respiratory Factor Advanced PF was shown to decrease transepidermal water loss by 29.24% and by 50.00% when compared to the untreated control after 4 weeks

• Promotes moisture retention

benefits when added to cosmetic applications

• 60.00%
• 50.00%
• 40.00%
• 30.00%
• 20.00%
• 10.00%

0.00%

Base Lotion vs. Untreated Experimental (2.0% AC DRF Advanced PF + Base Lotion) vs. Untreated Experimental (2.0% AC DRF Advanced PF + Base Lotion) vs. Base Lotion

Percent (%) Difference

Transepidermal Water Loss

Comparison of Test Sites - Water Loss Reduction Over Time

T = 0 T = 4 Weeks

Figure 14. Comparison of percent reduction in water loss over time between two test sites.

AC Dermal Respiratory Factor Advanced PF Efficacy Test Results

In-vitro Scratch Assay

Protocol

• Human dermal fibroblasts were

seeded into 6-well tissue culture plates

• 5% concentration of AC Dermal

Respiratory Factor Advanced PF was added to the serum-free DMEM and incubated with fibroblasts

• Scratch took place every 24 hours

and up to 96 hours

• AC Dermal Respiratory Factor

Advanced PF was able to increase cell migration and close the scratch at a rate comparable to the positive control

• Product has healing abilities and

cell proliferation properties

0.5 1 1.5 2 2.5 3 3.5 24 48 72 96

Scratch Area (mm2) Time Pose Scratch (Hours)

Area Enclosure

Positive Control Negative Control AC Dermal Respiratory Factor Advanced PF 20000 40000 60000 80000 100000 120000 24 48 72 96

Migration Rate (nm2/ hour) Time Post Scratch (Hours)

Migration Rate

Positive Control Negative Control AC Dermal Respiratory Factor Advanced PF

AC Dermal Respiratory Factor Advanced PF

Additional Data

• Dermal and Ocular Irritation Tests: Non-Irritating

AC Dermal Respiratory Factor Advanced PF

Technical Information:

Product Code: 20219PF INCI Name: Water + Saccharomyces Lysate Extract INCI Status: Conforms Suggested Use Levels: 0.5 – 1.0% Suggested Applications: Increases Cellular Respiration + Enhances Cellular Metabolism + Collagen/ Elastin Synthesis + Anti-Aging Solubility: Water Soluble

THANK YOU

For more information –Visit our website! www.activeconceptsllc.com

AC Dermal Respiratory Factor Advanced PF