18 April 2016 Jan Pen Centre of Expertise for Plant Compounds - - PowerPoint PPT Presentation

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18 April 2016 Jan Pen Centre of Expertise for Plant Compounds - - PowerPoint PPT Presentation

Nov 13, 2022 432 likes •680 views

Extract Library 18 April 2016 Jan Pen Centre of Expertise for Plant Compounds Background The Extract Library was set-up to provide a bridge between the huge biodiversity in plant compounds in Dutch horticultural crops and applications of

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Extract Library

18 April 2016 Jan Pen Centre of Expertise for Plant Compounds

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Background

The Extract Library was set-up to provide a bridge between the huge biodiversity in plant compounds in Dutch horticultural crops and applications of those crops in high-value non-traditional markets

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Market

Diverse market segments with a common need for novel products

Agro(chemicals) Pharmaceuticals Feed additives Cosmetics & toiletry Food supplements Fine chemicals Herbal medicines Natural colours, flavours and fragrances

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Market need

  • Biodiversity plant compounds
  • Information on cultivars and plant material

– Taxonomy, known compounds, (traditional) use, edibility, toxicity etc.

  • Reproducible supply
  • Cultivation without chemicals
  • Compliance with regulatory requirements
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Extract Library

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Crops

  • Huge diversity in plant types
  • Families:

205

  • Genera:

927 See list

  • Species:

1,300

  • Huge diversity in plant compounds
  • Estimate:

100,000 – 200,000

  • Can be grown in The Netherlands (or elsewhere)
  • Controlled and reproducible cultivation
  • Reproducible composition and amount of plant compounds
  • No chemical crop protection products used
  • No false positives
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Families & # of Genera

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Plant materials

– Plant materials: 2,245 – From one crop: 1-3 plant materials

  • Buds, beans, bulbs, branches, bark, cabbage, cress,

exudate, flowers, fruits, pods, leaves, mushrooms, nuts, roots, seeds, wood, stems, sprouts, substrate, tubers and combinations thereof – Maximally harvested: 250 g – Used for extraction: 50 g – Maximally available for e.g. follow-up R&D: 200 g

Amount available (g) Plant Materials (#) 35 < 50 53 50-100 154 100-150 166 150-200 1,837 Total 2,245

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Extracts

– Compliant with industry and regulatory requirements – Crude extracts: 2,244

  • Extraction solvent:

96.5% ethanol

  • Final solvent:
  • app. 89% ethanol*
  • Concentration:

1 ml corresponds with 200 mg plant material (f.w.)

– Cleaned extracts: 2,244

  • Sugars, fatty acids, bio-polymers, salts, amino acids and tannins removed
  • Final solvent:
  • app. 57% ethyl acetate, 23% ethanol, 20% water (v/v/v)*
  • Concentration:

1 ml corresponds with 200 mg plant material (f.w.)

*Can be supplied in dry form

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Extracts in plates

– Nature and form suitable for screening by various industries – Crude extracts: 2,240

  • Plate format:

96-deep well plates with removable tubes

Cherry picking possible

  • Extracts per plate:

80

  • Library (2,240 extracts):

28 plates

  • Content/well:

0.5 ml extract in app. 89% ethanol* Corresponds with 100 mg plant material (f.w.)

– Cleaned extracts: 2,240

  • Plate format:

384-deep well plates

Fixed format

  • Extracts per plate:

320

  • Library (2,240 extracts):

7 plates

  • Content/well:

0.1 ml dried-down extract Corresponds with 20 mg plant material (f.w.)

*Can be supplied in dry form

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Database

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Public information on plant species

– General

  • Known compounds
  • (Traditional) use
  • Edibility
  • Bioactivity
  • Toxicity

– Regulatory

  • CITES, EU Flora & Fauna, IUCN Red List, Dutch Food and Consumer law on

banned herbal products (Warenwetbesluit Kruidenmiddelen), EMA - THMP, EFSA toxic botanicals, Currently used and excluded cosmetic ingredients in IECIC China, EU banned and allowed cosmetic compounds

  • Plant Breeder’s Rights
  • Patents
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Database – Taxonomy

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Database – Regulatory

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Database – Literature

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Database – Compounds

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Screening Extract Library

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Screening results - I

– Pharmaceutical

  • HTS in an anti-inflammatory activity assay

– Cleaned extracts (960) in DMSO; 384-well format – A number of hits - to be validated

  • Anti-mycobacterium activity (TBC)

– Cleaned extracts (320) in ethanol; 384-well format – One hit - to be validated

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Screening results - II

– Agrobiological

  • Activity against an important agricultural fungal disease

– Cleaned extracts (960) converted to 96-well format in DMSO – Crude extracts (320) in 96-well format converted to DMSO – Several hits - to be validated

– Food

  • Activity against an important food spoiling fungus

– Cleaned extracts (960) converted to 96-well format in DMSO – Crude extracts (320) in 96-well format converted to DMSO – Several hits - to be validated

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Access Extract Library

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Products

– Extract Library

  • Extracts for screening – Crude or cleaned; Single or plated
  • Additional extract to e.g. validate hits
  • Plant material
  • Public information from the database

– Additional products

  • Preparation of extracts from plant species related to the “hit”
  • R&D - e.g. to increase the active component by breeding
  • Plant material for product development
  • Plant material for product
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Commercial terms

– Extracts for screening

  • Screening agreement
  • Blind screening – Extracts supplied under code
  • List of genera
  • Price per extract / plate
  • Success fee for hits that are pursued
  • Customer gets a first right to negotiate a license

– Public information from the database

  • Access fee for available data per plant species
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Contact us

  • Dr. Jan Pen

Project Manager Pompoenweg 9 2321 DK Leiden The Netherlands t: +31 6 53861090

  • e. jan.pen@plantenstoffen.nl